Standard name
Description GTPase-activating protein; negatively regulates RAS by converting it from GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; mutations cause catalase T deficiency, defective glycogen synthesis and defective trehalose accumulation; IRA1 has a paralog, IRA2, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.1 0.14 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.96 0.96 0.89 0.77 0.76 0.72 0.7 0.72 1.0 0.99 1.0 0.95 0.86 0.93 0.91 0.85 0.88 0.91 0.91 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.14 0 0.28 0.34 0.52 0.5 0.51 0.46 0 0 0 0 0 0 0 0 0.05 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 2 0 0 0 1 0 0 0 1 2 1 2 0 0 0 1 2
Bud 3 2 1 4 0 5 16 7 6 6 7 1 2 1 0 1 0 1 1 0 0 1 2
Bud Neck 0 0 0 0 1 0 6 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 1 1 1 2 2 0 0 0 0 0 0
Cell Periphery 2 1 3 1 3 2 2 7 2 4 10 5 7 20 17 15 25 0 0 0 0 0 0
Cytoplasm 208 93 109 212 302 337 436 227 246 204 205 266 343 422 190 131 170 205 98 98 75 227 230
Endoplasmic Reticulum 2 0 1 0 1 1 5 1 0 1 3 0 0 1 14 6 6 0 0 0 0 0 0
Endosome 0 0 0 0 3 1 0 0 0 0 0 0 0 0 3 9 0 0 2 0 0 0 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 4 0 0 0 0 1
Mitochondria 10 2 3 30 12 106 193 154 169 148 132 0 0 1 5 4 4 3 2 6 1 7 12
Nucleus 1 1 1 1 0 0 6 1 4 2 2 1 1 0 2 0 3 0 0 0 0 0 1
Nuclear Periphery 0 1 0 1 0 0 2 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 6 1 2 4 3 0 0 0 0 1 1 0 0 0 0 0 1
Vac/Vac Membrane 1 0 0 0 0 1 6 4 0 3 2 0 0 0 4 5 2 0 1 1 1 4 7
Unique Cell Count 217 95 112 220 313 378 563 297 340 292 285 267 345 423 201 152 183 225 115 112 83 249 274
Labelled Cell Count 228 100 118 249 324 457 682 405 431 375 367 273 353 445 236 177 215 225 115 112 83 249 274


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 6.5 3.4 3.7 4.2 3.4 3.6 3.4 3.5 3.5 3.4 5.1 5.4 5.2 6.9 6.7 6.8 4.6 5.2 5.4
Std Deviation (1e-4) 1.0 3.4 0.7 1.2 1.0 1.1 0.8 1.7 1.2 1.4 1.3 0.6 1.2 1.0 1.3 1.5 1.8 1.3 0.8 1.2
Intensity Change (Log2) 0.11 0.29 0.02 0.07 0.0 0.03 0.03 -0.0 0.58 0.66 0.63 1.02 0.99 1.01 0.43 0.61 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2.0 2.3 3.1
Cytoplasm -0.5 -0.4 -2.7 -4.9 -4.9 -5.6 -5.9 -5.6 2.0 1.9 2.7 -1.1 -3.1 -1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ira1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available