Standard name
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.06 0.05 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.09 0 0.05 0 0.07 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0.05 0.06 0.07 0.08 0.05 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.07 0.2 0.18 0.22 0.29 0.19 0.22 0.18 0.19 0.1 0.12 0.21 0.09 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0.08 0.21 0.06 0.05 0.05 0 0.06 0 0.11 0.16 0.18
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.09 0.09 0.06 0.1 0.39 0.54 0.32 0.7 0.55 0.78 0.54 0.06 0 0 0.09 0.07 0.16 0 0 0 0 0.05 0
Nucleus 0.61 0.68 0.77 0.16 0.31 0.15 0.11 0.08 0.1 0.13 0.11 0.09 0.39 0.33 0.27 0.41 0.44 0.3 0.32 0.39 0.34 0.15 0.09 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleolus 0.21 0.22 0.07 0.5 0.11 0.12 0.06 0.06 0.06 0.07 0.05 0 0.42 0.4 0.21 0.39 0.5 0.54 0.4 0.36 0.34 0.38 0.29 0.27
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.16 0.69 0.37 0.37 0.32 0.35 0.23 0.27 0.16 0.29 0.44 0.51 0.47 0.2 0.07 0.1 0.06 0 0.09 0.22 0.22 0.22
Vac/Vac Membrane 0.05 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.07 0.07 0.09 0.08 0.06 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2
Bud 0 0 1 3 3 7 15 13 14 17 15 18 2 1 1 3 0 0 3 1 1 2 7 11
Bud Neck 0 0 0 16 9 12 8 15 8 15 7 15 6 22 9 0 0 0 0 1 2 3 12 12
Bud Site 1 0 1 2 7 13 14 13 19 23 14 14 2 2 0 0 0 2
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0
Cytoplasm 7 8 8 13 40 41 56 65 53 63 52 48 29 90 116 17 5 12 1 2 1 1 10 18
Endoplasmic Reticulum 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 7 3 3 0 0 0 0 2 2
Endosome 0 2 1 6 14 14 4 4 3 1 1 1 9 60 117 12 6 9 16 11 4 35 87 103
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 2 5 1 1 0 1 1 4
Mitochondria 183 15 13 11 21 89 140 73 198 155 227 136 17 10 12 18 8 28 7 0 4 7 27 21
Nucleus 228 109 107 28 63 34 28 19 28 38 33 23 117 245 151 79 52 51 125 78 55 48 49 31
Nuclear Periphery 11 3 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 23 2 5 0 8 8
Nucleolus 78 35 10 88 22 28 16 13 16 19 15 8 125 298 121 76 59 93 157 72 54 123 159 155
Peroxisomes 0 0 0 0 1 1 2 0 1 0 0 1 0 2 7 0 1 0 0 0 0 0 2 1
SpindlePole 7 2 22 122 76 85 83 80 65 75 48 74 133 382 267 39 8 17 23 6 15 73 120 129
Vac/Vac Membrane 19 8 3 1 10 6 3 3 4 4 8 6 2 4 13 11 6 12 29 18 13 20 40 52
Unique Cell Count 373 160 139 177 203 231 259 227 281 283 292 254 300 742 564 195 118 172 394 198 161 326 547 581
Labelled Cell Count 535 182 167 290 267 330 369 300 409 410 420 344 442 1120 816 267 150 233 394 198 161 326 547 581


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.9 5.2 5.3 5.5 4.3 3.8 4.4 3.2 3.5 3.1 3.5 5.5 5.6 5.5 8.2 7.7 7.3 5.9 5.6 6.1
Std Deviation (1e-4) 0.6 1.2 0.8 1.7 2.6 2.3 2.0 2.0 0.9 1.0 0.9 0.7 1.1 1.0 1.9 1.7 1.7 1.9 1.5 1.9 2.0
Intensity Change (Log2) 0.02 0.07 -0.28 -0.44 -0.24 -0.7 -0.55 -0.75 -0.58 0.08 0.11 0.08 0.66 0.57 0.5 0.18 0.12 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 3.6 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 3.6 3.3 4.1 5.3 3.6 4.3 3.4 3.6 1.4 2.2 4.1 1.0 -0.6 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.7 0 0 0 0 0 0 0 0 3.1 5.7 2.5 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 0.3 0 0 0 0 0 0 0 -1.4 0 0 0 -0.8 1.8
Nucleus -10.9 -8.3 -11.9 -13.3 -13.4 -13.8 -12.9 -13.6 -13.7 -7.4 -9.7 -11.0 -6.6 -5.4 -8.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 8.1 1.1 0 0 0 0 0 0 0 7.3 7.5 3.9 6.5 7.7 8.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 9.4 4.3 0 0 0 0 0 0 0 5.8 7.7 6.8 1.0 -2.3 -1.6
Vacuole 0 1.3 0 0 0 0 0 0 0 0 0 0 1.6 0 2.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bmt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available