Standard name
Human Ortholog
Description Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec72p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.11 0.16 0.28 0.17 0.12 0.13 0.1 0.14 0.14 0.16 0.17 0.11 0.12 0.13 0.22 0.18 0.16 0 0 0 0 0.07 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.11 0.11 0.19 0.21 0.11 0.08 0.08 0.08 0.12 0.11 0.16 0.14 0.17 0.31 0.45 0.46 0.48 0.55 0 0 0.06 0 0 0.09
Cytoplasm 0.05 0.13 0 0 0.08 0.07 0.05 0 0.23 0.06 0.1 0.1 0.07 0.06 0 0.19 0.2 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.85 0.83 0.82 0.74 0.72 0.66 0.69 0.62 0.4 0.55 0.45 0.46 0.8 0.72 0.73 0 0 0 0.88 0.9 0.89 0.77 0.79 0.83
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.07 0.15 0.05 0 0 0 0 0 0 0 0 0 0 0.22 0.17 0.15 0 0 0 0 0 0
Mitochondria 0 0 0.16 0.29 0.2 0.27 0.35 0.3 0.37 0.43 0.5 0.37 0 0 0 0.12 0.08 0.12 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0 0.08 0.13 0.05 0.06 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 46 67 90 63 57 81 64 60 76 75 81 21 20 24 39 27 18 1 9 9 4 22 5
Bud 0 3 9 3 15 18 17 12 23 19 25 14 1 0 0 4 0 0 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 20 47 78 68 43 37 52 51 52 64 77 69 32 51 80 83 73 62 6 7 26 6 12 26
Cytoplasm 9 54 15 11 29 34 34 29 100 31 49 48 14 10 8 34 30 19 4 11 0 1 3 2
Endoplasmic Reticulum 161 340 334 241 268 319 429 406 175 305 217 225 153 119 130 2 2 1 176 395 369 118 243 241
Endosome 0 0 0 0 0 2 0 0 1 2 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 2 3 30 50 18 8 3 8 2 3 4 2 0 4 1 39 25 17 0 2 3 6 7 1
Mitochondria 4 6 67 93 73 132 220 194 162 240 237 182 1 0 2 21 12 14 1 1 4 9 9 6
Nucleus 0 0 0 0 0 0 2 1 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 1 2 11 25 23 7 22 13 10 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 0 5 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 3 0 2 13 39 20 54 58 27 29 56 1 1 2 1 3 0 5 4 0 2 6 3
Unique Cell Count 189 412 407 326 374 487 620 655 442 557 477 486 192 166 179 179 151 112 201 437 416 154 309 291
Labelled Cell Count 219 502 602 559 524 658 883 847 641 789 729 689 223 206 247 223 175 131 201 437 416 154 309 291


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.4 19.8 17.9 16.5 13.5 11.9 11.2 11.7 12.3 11.0 10.7 10.8 18.6 18.3 19.5 21.6 21.7 21.5 17.5 17.2 18.2
Std Deviation (1e-4) 3.2 3.3 2.7 2.8 2.4 2.0 1.9 2.0 4.9 1.9 2.0 2.1 3.5 2.9 3.1 5.5 5.4 5.3 3.3 3.1 3.1
Intensity Change (Log2) – – – -0.11 -0.4 -0.58 -0.67 -0.62 -0.54 -0.7 -0.74 -0.73 0.06 0.03 0.13 0.27 0.28 0.27 -0.03 -0.06 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.7 0.1 -2.0 -1.5 -3.2 -1.2 -1.2 -0.3 0.1 -1.8 -1.3 -0.9 1.5 0.4 -0.1
Bud -1.4 1.5 1.3 0.5 -0.4 2.3 1.1 2.3 0.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 -3.0 -5.1 -5.1 -5.5 -3.0 -3.3 -1.2 -2.0 -0.7 3.0 6.4 6.8 6.9 7.6
Cytoplasm -0.2 2.5 2.2 1.3 0.6 8.1 1.4 3.8 3.6 1.9 1.2 0.5 6.2 6.2 5.0
Endoplasmic Reticulum -2.7 -3.5 -5.6 -4.6 -6.9 -12.6 -8.9 -11.2 -11.0 -0.7 -2.8 -2.6 -18.2 -17.3 -15.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.4 -1.5 -4.2 -6.1 -5.2 -5.3 -5.8 -5.0 -5.6 -3.9 -2.3 -3.4 5.0 3.2 2.5
Mitochondria 3.9 1.1 3.8 6.6 4.8 6.6 8.8 10.4 7.0 -5.7 -5.6 -5.3 -1.5 -2.6 -1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.6 3.8 3.5 0 3.7 2.9 2.4 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.8 5.8 3.7 5.9 7.6 4.5 5.1 7.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.3177 15.0824 15.4924 14.6337 14.8542 14.7411 20.5532 19.2511 19.6456 18.7675 18.1808 19.5068 19.7571 19.4128 19.1463 17.3164 15.6325 18.8012
Actin 0.0041 0.0074 0.0024 0.0131 0.0003 0.0021 0.0055 0.0004 0.0016 0.0001 0.0003 0.0002 0.0088 0.0006 0.0051 0.0233 0.0401 0.0002
Bud 0.0001 0.0003 0 0.0001 0 0 0.0001 0 0.0001 0 0 0 0.0004 0 0.0002 0.0006 0.0008 0
Bud Neck 0.0004 0.001 0.0006 0.0072 0.01 0.0012 0.0002 0.0001 0.0013 0.0001 0.0006 0.0008 0.0008 0.0003 0.0011 0.0007 0.0007 0.0009
Bud Periphery 0.0006 0.0009 0.0004 0.0006 0.0003 0.0004 0.0002 0.0001 0.0008 0.0001 0.0001 0.0001 0.0009 0.0001 0.001 0.0007 0.0013 0.0001
Bud Site 0.0002 0.0038 0.0001 0.0008 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0052 0.0001 0.0012 0.0036 0.0022 0
Cell Periphery 0.0192 0.0373 0.0208 0.0176 0.0123 0.0082 0.0111 0.0114 0.0367 0.0134 0.0053 0.0064 0.0201 0.021 0.0224 0.0095 0.0059 0.007
Cytoplasm 0.0227 0.0155 0.0109 0.0035 0.0161 0.0061 0.0097 0.011 0.0059 0.0031 0.0021 0.003 0.0306 0.0108 0.0167 0.003 0.0171 0.0047
Cytoplasmic Foci 0.0005 0.0007 0.0006 0.001 0.0001 0.0003 0.0009 0.0005 0.0003 0.0001 0.0002 0.0002 0.0127 0.0003 0.0033 0.0131 0.0041 0.0002
Eisosomes 0.0017 0.0095 0.0008 0.0022 0.0007 0.0011 0.0004 0.0002 0.0026 0.0001 0.0006 0.0003 0.002 0.0002 0.0005 0.0003 0.0004 0.0003
Endoplasmic Reticulum 0.9295 0.8959 0.9425 0.9329 0.9539 0.9691 0.9437 0.9468 0.9324 0.9757 0.9756 0.9819 0.8726 0.9369 0.9103 0.8934 0.8325 0.9761
Endosome 0.0017 0.0013 0.0011 0.0026 0.0003 0.0018 0.0059 0.0012 0.0015 0.0003 0.0014 0.0009 0.0061 0.0013 0.007 0.0135 0.0058 0.0009
Golgi 0.0042 0.0042 0.0083 0.0083 0.002 0.005 0.0037 0.0021 0.0054 0.0005 0.0073 0.0022 0.006 0.0029 0.0107 0.0148 0.009 0.0026
Lipid Particles 0.003 0.0046 0.0054 0.0025 0.0005 0.001 0.0045 0.0221 0.0036 0.0023 0.0009 0.0014 0.0078 0.0159 0.0059 0.0055 0.0418 0.0023
Mitochondria 0.0003 0.006 0.0002 0.0008 0.0001 0.0002 0.0052 0.0001 0.0006 0.0001 0.0003 0.0001 0.0021 0.0002 0.0038 0.0087 0.0261 0.0001
None 0.0003 0.0002 0.0001 0.0002 0 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0.0016 0 0.0002 0.0003 0.0004 0
Nuclear Periphery 0.0089 0.0044 0.0033 0.0035 0.0021 0.0021 0.0044 0.0022 0.004 0.0023 0.0036 0.0016 0.0126 0.0051 0.0061 0.0034 0.003 0.0027
Nucleolus 0 0.0025 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0001 0.0001 0
Nucleus 0.0002 0.0012 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0018 0.0002 0.0003 0.0002 0.0004 0.0001
Peroxisomes 0 0.0003 0 0.0001 0 0 0.0014 0 0 0 0 0 0.0022 0 0.0005 0.0024 0.0057 0
Punctate Nuclear 0 0.0005 0 0.0001 0 0 0.0001 0 0 0 0 0 0.0006 0 0.0001 0.0011 0.0003 0
Vacuole 0.0018 0.002 0.002 0.0021 0.0008 0.0009 0.0018 0.0014 0.0025 0.0015 0.0012 0.0008 0.0043 0.0038 0.0029 0.0014 0.0016 0.0016
Vacuole Periphery 0.0005 0.0006 0.0002 0.0005 0.0002 0.0002 0.0005 0.0001 0.0004 0.0001 0.0003 0.0001 0.0007 0.0002 0.0007 0.0004 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 88.8409 41.2976 53.0387 69.0406 67.9598 72.8092 56.6114 54.7389 72.1405 73.8713
Translational Efficiency 1.9021 2.8657 2.2376 2.3038 2.0399 2.2666 1.8236 1.9105 1.7054 1.6313

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘