Standard name
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0.07 0.05 0.05 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.07 0.05 0.07 0.07 0.05 0.06 0.07 0.07 0.09 0.09 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.05 0.05 0.05 0 0.05 0 0 0.05 0.1 0.15 0.16 0.07 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.07 0 0 0 0 0 0 0 0 0 0 0.15 0.09 0.15 0.15 0.11 0.12 0.06 0 0 0.08 0.06 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0.12 0.11 0.15 0.12 0.13 0.11 0.18 0.12 0.12 0.14 0 0 0 0 0.09 0 0 0 0 0 0 0
Nucleus 0.07 0 0.06 0 0 0 0.05 0 0.05 0.05 0.05 0 0 0.05 0 0 0.05 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.49 0.46 0.36 0.37 0.4 0.31 0.33 0.36 0.41 0.35 0.44 0.38 0.45 0.45 0.43 0.67 0.64 0.59 0.06 0 0 0 0.06 0.05
Peroxisomes 0 0.06 0 0.1 0 0.09 0.06 0.05 0 0.05 0 0.07 0.08 0.05 0.06 0.1 0.05 0.08 0 0 0 0 0 0
SpindlePole 0.7 0.72 0.86 0.7 0.65 0.75 0.65 0.7 0.68 0.72 0.71 0.74 0.68 0.61 0.65 0.64 0.63 0.66 0.78 0.8 0.86 0.74 0.71 0.65
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 2 0 0 1 2
Bud 3 4 28 9 12 18 20 16 13 16 10 8 0 2 1 0 1 1 14 17 2 4 8 8
Bud Neck 12 10 14 9 15 12 10 14 6 18 7 3 4 14 9 1 1 2 8 13 1 1 3 8
Bud Site 15 8 30 17 12 27 18 22 23 31 19 15 2 3 5 1 0 1
Cell Periphery 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 20 30 8 17 9 21 18 13 14 14 6 9 40 86 104 13 15 29 1 0 0 0 1 1
Endoplasmic Reticulum 0 0 0 1 0 0 1 1 0 0 0 0 1 1 0 1 1 2 0 0 0 0 2 1
Endosome 22 31 2 2 0 3 4 6 4 2 2 3 60 54 97 29 20 34 34 23 21 26 31 32
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 1 3 2 4
Mitochondria 46 6 53 39 26 46 48 40 55 54 26 24 1 4 3 5 15 6 1 3 3 3 2 6
Nucleus 35 12 26 14 3 10 18 13 14 24 11 5 16 29 24 4 8 14 8 1 1 2 6 4
Nuclear Periphery 2 3 1 1 0 0 1 1 2 0 3 1 0 0 1 0 0 0 0 1 0 0 2 1
Nucleolus 260 219 167 129 69 121 126 134 127 154 98 65 181 259 285 132 112 174 34 16 21 9 32 22
Peroxisomes 18 30 10 35 4 35 24 17 13 23 7 12 30 30 41 19 8 23 3 8 2 4 4 10
SpindlePole 370 342 395 245 111 296 250 264 208 317 156 128 271 351 432 125 109 192 435 424 411 245 355 297
Vac/Vac Membrane 5 10 0 0 0 1 1 1 0 0 0 0 3 10 6 3 2 6 1 3 0 3 6 5
Unique Cell Count 532 474 460 350 172 394 383 376 308 440 221 173 398 575 666 196 174 293 556 533 479 330 502 458
Labelled Cell Count 809 706 734 519 261 591 539 542 479 653 346 273 609 844 1009 334 293 487 556 533 479 330 502 458


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.5 4.0 4.3 3.2 4.1 4.3 4.6 4.0 4.6 4.4 4.5 7.5 6.8 7.0 10.4 10.0 10.1 7.2 7.3 7.3
Std Deviation (1e-4) 1.2 1.8 0.8 1.3 0.9 1.1 1.5 1.5 1.3 1.5 1.5 1.5 1.9 1.7 1.9 2.2 2.2 2.5 1.8 1.6 1.6
Intensity Change (Log2) 0.08 -0.32 0.03 0.08 0.2 -0.01 0.18 0.11 0.16 0.88 0.76 0.79 1.37 1.31 1.32 0.83 0.86 0.86


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -5.0 -5.5 -6.2 -3.5 -3.0 -4.0
Bud Neck 0 0 0 0 0 0 0 0 0 -2.1 -0.6 -2.0 0 0 -2.2
Bud Site 0 0 0 0 0 0 0 0 0 -4.6 -5.5 -5.5 -3.3 -3.5 -4.2
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.5 0 2.9 2.5 1.6 2.3 1.4 0 0 5.3 7.4 7.6 3.3 4.1 5.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 8.3 6.3 8.2 7.9 6.8 7.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.8 -7.6 -8.4 -3.7 -1.1 -4.7
Nucleus -1.1 -2.1 -2.3 -0.6 -1.5 -0.7 -0.1 -0.4 -1.4 -1.1 -0.4 -1.6 -2.0 -0.5 -0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 2.7 2.8 2.2 7.3 6.4 6.2
Peroxisomes 0 0 0 0 0 0 0 0 0 3.7 2.5 3.2 4.3 0 3.7
SpindlePole 0 0 0 0 0 0 0 0 0 -6.2 -8.8 -7.8 -6.4 -6.5 -6.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dad3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available