Standard name
Description Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0.06 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.97 0.98 0.91 0.93 – 0.8 0.84 0.77 0.77 0.99 0.99 0.99 0.95 0.89 0.9 0.79 0.85 0.65 0.78 0.77 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.06 – 0.27 0.21 0.31 0.28 0 0 0 0 0 0.08 0.08 0 0.17 0.08 0.07 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 4 3 4 4 2 2
Bud 0 1 1 0 0 4 0 – 5 5 6 13 0 0 1 0 0 0 4 2 1 3 10 12
Bud Neck 0 3 15 0 14 12 14 – 12 9 12 12 0 0 0 0 0 0 2 2 2 1 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 0 2 2 2 – 3 2 2 1 1 1 0 1 2 0 1 1 4 1 1 2
Cytoplasm 285 253 241 66 230 275 344 – 323 324 317 342 286 325 370 69 68 53 232 225 162 182 260 187
Endoplasmic Reticulum 1 0 0 0 0 0 1 – 4 0 0 1 3 1 1 7 3 3 0 0 3 0 3 1
Endosome 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 3 1 1 5 2 4 2 5 2
Golgi 0 1 0 0 1 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 2 1 1
Mitochondria 6 10 2 0 2 41 22 – 109 81 126 127 0 1 0 3 2 5 24 11 42 19 22 10
Nucleus 0 0 1 1 1 8 2 – 7 4 2 2 0 0 1 1 1 3 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 – 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 2 1 3 2 14 – 15 14 10 12 0 0 0 0 1 0 3 4 8 4 6 5
Vac/Vac Membrane 2 0 1 0 0 0 1 – 0 3 1 2 0 0 0 1 2 1 4 3 1 2 9 6
Unique Cell Count 292 261 249 68 234 301 371 404 387 411 446 288 327 372 73 76 59 294 266 252 235 337 242
Labelled Cell Count 296 270 266 68 253 345 401 479 443 478 516 291 328 374 85 81 66 294 266 252 235 337 242


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.6 4.4 3.9 4.7 3.5 4.2 – 3.4 3.7 3.4 3.5 4.6 4.1 4.7 5.3 5.9 5.6 4.2 4.4 4.7
Std Deviation (1e-4) 0.5 0.7 1.3 1.0 1.7 1.7 1.8 – 1.2 1.3 1.0 1.0 1.6 1.5 1.6 1.4 2.3 1.9 2.1 2.2 2.3
Intensity Change (Log2) – – – -0.15 0.12 -0.32 -0.07 – -0.36 -0.25 -0.35 -0.31 0.08 -0.09 0.11 0.29 0.44 0.35 -0.07 0.01 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rib5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available