Standard name
Description Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.09 0.16 0.06 0.15 0.13 0.09 – 0.17 0.19 0.21 0 0 0 0.15 0.13 0 0 0 0.05 0.06 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0.05 0 0.07 0.07 0.05 – 0.06 0.09 0.13 0.11 0.11 0.17 0.32 0.11 0.21 0 0 0 0 0.05 0.06
Cytoplasm 0 0.14 0 0 0.11 0.07 0.07 0.06 – 0.07 0.11 0.06 0.07 0.07 0.11 0.24 0.29 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0.92 0.84 0.88 0.71 0.61 0.78 0.77 0.8 – 0.71 0.59 0.63 0.88 0.89 0.82 0.07 0 0.09 0.93 0.87 0.91 0.76 0.86 0.82
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.15 0 0.28 0.42 0.31 0.13 0.12 0.08 – 0.06 0.07 0.1 0 0 0 0.31 0.36 0.51 0 0 0 0.05 0 0
Mitochondria 0 0 0 0.09 0 0.1 0.08 0.08 – 0.23 0.31 0.26 0 0 0 0.17 0.11 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 13 39 53 19 69 72 46 – 81 78 111 7 7 7 15 16 1 6 5 20 9 6 8
Bud 0 1 3 5 5 3 5 5 – 5 9 11 0 0 0 0 3 0 0 2 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 15 9 25 18 11 31 39 26 – 29 37 70 28 28 36 32 13 11 3 2 4 5 12 17
Cytoplasm 11 35 13 14 34 31 37 32 – 33 47 32 17 17 23 24 34 13 2 10 1 3 2 3
Endoplasmic Reticulum 359 209 373 240 190 350 420 404 – 329 240 343 222 220 174 7 4 5 374 239 388 113 239 245
Endosome 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 1 0 0 0 1 0 1 0 1
Golgi 59 1 121 140 95 58 67 41 – 30 28 56 8 8 3 31 43 27 4 1 6 6 8 9
Mitochondria 0 1 4 31 13 45 41 39 – 106 127 140 0 0 0 17 13 7 3 3 1 4 6 7
Nucleus 0 1 0 0 2 0 0 0 – 0 0 0 0 0 2 1 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 4 10 17 – 7 8 21 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 6 0 2 2 – 3 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 2 4 3 25 5 22 17 – 18 8 20 3 2 5 0 11 2 1 3 0 2 0 1
Unique Cell Count 390 248 425 336 311 451 544 504 466 409 541 251 247 212 101 119 53 401 275 429 150 280 301
Labelled Cell Count 456 272 583 504 401 596 715 629 641 582 804 285 282 250 128 140 66 401 275 429 150 280 301


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 47.1 39.5 35.8 35.0 27.9 26.5 25.2 23.9 – 20.2 19.6 21.0 42.8 42.8 41.2 25.0 25.5 35.7 40.1 46.1 50.4
Std Deviation (1e-4) 8.7 10.3 7.2 7.2 10.7 5.4 5.0 4.9 – 4.8 4.6 4.7 10.2 10.2 11.5 8.1 10.1 12.3 10.0 9.1 9.8
Intensity Change (Log2) – – – -0.03 -0.36 -0.44 -0.51 -0.58 – -0.82 -0.87 -0.77 0.26 0.26 0.2 -0.52 -0.49 -0.01 0.16 0.36 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.8 -1.5 2.8 2.0 0 – 3.6 4.1 4.8 -3.2 -3.1 -2.7 1.7 1.4 0
Bud 0 0 0 0 0 – 0 1.8 1.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.3 -1.5 0.6 0.8 -0.5 – 0.2 1.7 3.7 2.5 2.5 4.5 7.5 1.9 0
Cytoplasm 0.8 4.3 2.6 2.6 2.3 – 2.7 4.7 2.1 2.3 2.3 4.0 7.3 8.8 0
Endoplasmic Reticulum -5.7 -8.4 -4.0 -4.2 -3.1 – -6.3 -9.5 -8.6 0.3 0.5 -1.9 -16.3 -17.6 -13.2
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 3.8 0.6 -5.7 -6.3 -8.1 – -8.8 -8.1 -7.2 -8.1 -8.0 -8.1 0.4 1.6 3.3
Mitochondria 5.4 2.9 5.8 4.8 4.9 – 9.9 11.9 10.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.5 – 0 0 3.8 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 4.9 0 3.0 2.5 – 2.8 1.2 2.7 0 0 0 0 0 0