Standard name | |
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Human Ortholog | |
Description | Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 13 | 39 | 53 | 19 | 69 | 72 | 46 | – | 81 | 78 | 111 | 7 | 7 | 7 | 15 | 16 | 1 | 6 | 5 | 20 | 9 | 6 | 8 |
Bud | 0 | 1 | 3 | 5 | 5 | 3 | 5 | 5 | – | 5 | 9 | 11 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 15 | 9 | 25 | 18 | 11 | 31 | 39 | 26 | – | 29 | 37 | 70 | 28 | 28 | 36 | 32 | 13 | 11 | 3 | 2 | 4 | 5 | 12 | 17 |
Cytoplasm | 11 | 35 | 13 | 14 | 34 | 31 | 37 | 32 | – | 33 | 47 | 32 | 17 | 17 | 23 | 24 | 34 | 13 | 2 | 10 | 1 | 3 | 2 | 3 |
Endoplasmic Reticulum | 359 | 209 | 373 | 240 | 190 | 350 | 420 | 404 | – | 329 | 240 | 343 | 222 | 220 | 174 | 7 | 4 | 5 | 374 | 239 | 388 | 113 | 239 | 245 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Golgi | 59 | 1 | 121 | 140 | 95 | 58 | 67 | 41 | – | 30 | 28 | 56 | 8 | 8 | 3 | 31 | 43 | 27 | 4 | 1 | 6 | 6 | 8 | 9 |
Mitochondria | 0 | 1 | 4 | 31 | 13 | 45 | 41 | 39 | – | 106 | 127 | 140 | 0 | 0 | 0 | 17 | 13 | 7 | 3 | 3 | 1 | 4 | 6 | 7 |
Nucleus | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 4 | 10 | 17 | – | 7 | 8 | 21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 6 | 0 | 2 | 2 | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 2 | 4 | 3 | 25 | 5 | 22 | 17 | – | 18 | 8 | 20 | 3 | 2 | 5 | 0 | 11 | 2 | 1 | 3 | 0 | 2 | 0 | 1 |
Unique Cell Count | 390 | 248 | 425 | 336 | 311 | 451 | 544 | 504 | 466 | 409 | 541 | 251 | 247 | 212 | 101 | 119 | 53 | 401 | 275 | 429 | 150 | 280 | 301 | |
Labelled Cell Count | 456 | 272 | 583 | 504 | 401 | 596 | 715 | 629 | 641 | 582 | 804 | 285 | 282 | 250 | 128 | 140 | 66 | 401 | 275 | 429 | 150 | 280 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 47.1 | 39.5 | 35.8 | 35.0 | 27.9 | 26.5 | 25.2 | 23.9 | – | 20.2 | 19.6 | 21.0 | 42.8 | 42.8 | 41.2 | 25.0 | 25.5 | 35.7 | 40.1 | 46.1 | 50.4 |
Std Deviation (1e-4) | 8.7 | 10.3 | 7.2 | 7.2 | 10.7 | 5.4 | 5.0 | 4.9 | – | 4.8 | 4.6 | 4.7 | 10.2 | 10.2 | 11.5 | 8.1 | 10.1 | 12.3 | 10.0 | 9.1 | 9.8 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.36 | -0.44 | -0.51 | -0.58 | – | -0.82 | -0.87 | -0.77 | 0.26 | 0.26 | 0.2 | -0.52 | -0.49 | -0.01 | 0.16 | 0.36 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.8 | -1.5 | 2.8 | 2.0 | 0 | – | 3.6 | 4.1 | 4.8 | -3.2 | -3.1 | -2.7 | 1.7 | 1.4 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 1.8 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.3 | -1.5 | 0.6 | 0.8 | -0.5 | – | 0.2 | 1.7 | 3.7 | 2.5 | 2.5 | 4.5 | 7.5 | 1.9 | 0 |
Cytoplasm | 0.8 | 4.3 | 2.6 | 2.6 | 2.3 | – | 2.7 | 4.7 | 2.1 | 2.3 | 2.3 | 4.0 | 7.3 | 8.8 | 0 |
Endoplasmic Reticulum | -5.7 | -8.4 | -4.0 | -4.2 | -3.1 | – | -6.3 | -9.5 | -8.6 | 0.3 | 0.5 | -1.9 | -16.3 | -17.6 | -13.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.8 | 0.6 | -5.7 | -6.3 | -8.1 | – | -8.8 | -8.1 | -7.2 | -8.1 | -8.0 | -8.1 | 0.4 | 1.6 | 3.3 |
Mitochondria | 5.4 | 2.9 | 5.8 | 4.8 | 4.9 | – | 9.9 | 11.9 | 10.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3.5 | – | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.9 | 0 | 3.0 | 2.5 | – | 2.8 | 1.2 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 54.1098 | 46.4739 | 49.9848 | 48.6 | 47.8981 | 49.4005 | 45.6975 | 52.4934 | 48.049 | 42.1503 | 39.0449 | 48.3323 | 54.5072 | 53.1437 | 53.105 | 47.5806 | 46.5621 | 50.4022 |
Actin | 0.0007 | 0.0014 | 0.0011 | 0.0001 | 0.0005 | 0.0009 | 0.0088 | 0.0003 | 0.0012 | 0.0073 | 0.051 | 0.0077 | 0.0234 | 0.0002 | 0.0012 | 0.0066 | 0.0011 | 0.0028 |
Bud | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0004 | 0.0006 | 0.0039 | 0.0004 | 0.0022 | 0.0014 | 0.0002 | 0.0002 | 0.0002 | 0.0008 | 0.003 | 0.0018 | 0.0006 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0004 |
Bud Periphery | 0.0002 | 0.0006 | 0.0012 | 0 | 0.0003 | 0.0003 | 0.0004 | 0.0012 | 0.0003 | 0.0012 | 0.0007 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0003 | 0.0011 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0033 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0548 | 0.0868 | 0.0601 | 0.0492 | 0.0413 | 0.0264 | 0.0173 | 0.0349 | 0.0155 | 0.0191 | 0.0125 | 0.0061 | 0.024 | 0.0469 | 0.0259 | 0.0162 | 0.0031 | 0.004 |
Cytoplasm | 0.0343 | 0.0601 | 0.0087 | 0.0069 | 0.0113 | 0.0129 | 0.0067 | 0.0042 | 0.0019 | 0.0016 | 0.0021 | 0.0018 | 0.0144 | 0.0071 | 0.0049 | 0.0007 | 0.0011 | 0.0011 |
Cytoplasmic Foci | 0.0022 | 0.0011 | 0.0007 | 0.0001 | 0.0006 | 0.0005 | 0.0048 | 0.0001 | 0.003 | 0.002 | 0.018 | 0.0005 | 0.0101 | 0.0002 | 0.0011 | 0.0038 | 0.0011 | 0.001 |
Eisosomes | 0.0025 | 0.0042 | 0.0038 | 0.0005 | 0.0012 | 0.0031 | 0.0033 | 0.0041 | 0.0012 | 0.007 | 0.0092 | 0.0008 | 0.0054 | 0.003 | 0.0017 | 0.0009 | 0.0046 | 0.0022 |
Endoplasmic Reticulum | 0.8159 | 0.7525 | 0.8496 | 0.8604 | 0.8446 | 0.8901 | 0.8296 | 0.9112 | 0.9205 | 0.833 | 0.759 | 0.917 | 0.789 | 0.883 | 0.9062 | 0.8756 | 0.9063 | 0.9429 |
Endosome | 0.0008 | 0.0021 | 0.0031 | 0.0005 | 0.005 | 0.0023 | 0.0055 | 0.001 | 0.0023 | 0.0038 | 0.0275 | 0.0036 | 0.0051 | 0.0014 | 0.0056 | 0.0049 | 0.0088 | 0.0028 |
Golgi | 0.001 | 0.0047 | 0.0051 | 0.0011 | 0.0435 | 0.0077 | 0.0063 | 0.003 | 0.0113 | 0.0254 | 0.0284 | 0.023 | 0.0062 | 0.0195 | 0.0055 | 0.0235 | 0.0275 | 0.0181 |
Lipid Particles | 0.0209 | 0.0419 | 0.0331 | 0.0327 | 0.0167 | 0.0209 | 0.0053 | 0.0078 | 0.0039 | 0.0095 | 0.0299 | 0.0037 | 0.0122 | 0.0119 | 0.005 | 0.0084 | 0.0087 | 0.0011 |
Mitochondria | 0.0042 | 0.0006 | 0.0014 | 0 | 0.0004 | 0.0005 | 0.0182 | 0.0126 | 0.0139 | 0.0564 | 0.0053 | 0.008 | 0.0049 | 0.0013 | 0.0052 | 0.0127 | 0.0061 | 0.0011 |
None | 0.0015 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0015 | 0.0006 | 0.0022 | 0.0039 | 0.0038 | 0.0001 | 0.006 | 0.0001 | 0.0004 | 0.0002 | 0.0015 | 0.0018 |
Nuclear Periphery | 0.035 | 0.0187 | 0.0148 | 0.0375 | 0.0175 | 0.0245 | 0.0644 | 0.0096 | 0.0123 | 0.0155 | 0.0232 | 0.0192 | 0.0751 | 0.0139 | 0.0283 | 0.0219 | 0.0178 | 0.0144 |
Nucleolus | 0.0039 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0021 | 0.0008 | 0.0006 | 0 | 0.0015 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.001 |
Nucleus | 0.003 | 0.0016 | 0.0005 | 0.0011 | 0.0009 | 0.001 | 0.0032 | 0.0004 | 0.0008 | 0.0008 | 0.0008 | 0.0003 | 0.0028 | 0.0002 | 0.0003 | 0.0003 | 0.0007 | 0.0012 |
Peroxisomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0.0006 | 0.0008 | 0.0059 | 0.0012 | 0.0011 | 0 | 0.0002 | 0.0187 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0011 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.008 | 0 | 0.002 | 0.0005 | 0.0028 | 0 | 0.0034 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0019 |
Vacuole | 0.0161 | 0.0197 | 0.0111 | 0.0083 | 0.0112 | 0.0059 | 0.0077 | 0.0048 | 0.0022 | 0.0028 | 0.0053 | 0.0023 | 0.0074 | 0.0085 | 0.0045 | 0.0027 | 0.0039 | 0.001 |
Vacuole Periphery | 0.0013 | 0.0008 | 0.0009 | 0.0009 | 0.0022 | 0.0011 | 0.0065 | 0.0037 | 0.002 | 0.0074 | 0.0073 | 0.0025 | 0.006 | 0.0022 | 0.003 | 0.0018 | 0.0066 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 185.0244 | 158.7041 | 152.5483 | 209.9723 | 175.7914 | 131.0176 | 170.9601 | 178.4493 | 171.5088 | 195.6213 |
Translational Efficiency | 0.9551 | 1.0362 | 1.2762 | 0.7968 | 0.8548 | 1.3104 | 1.0045 | 0.814 | 0.8994 | 0.8584 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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