Standard name
Description Nucleolar protein required for rRNA synthesis and ribosomal assembly; required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit; essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0.07 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0.05 0 0 0.07 0.06 0.05 0.08 0.07 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.09 0.09 0.09 0.1 0.13 0.14 0.15 0.12 0.11 0.11 0.14 0.2 0.3 0.08 0.13 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0.11 0.13 0.07 0 0 0 0 0 0 0.08 0.11 0.08 0.09 0.08 0.1 0.1 0.09 0.11 0.13 0.17
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.65 0.13 0.12 0.09 0.06 0.3 0.54 0.44 0.64 0.6 0.75 0.58 0 0 0 0.09 0.12 0.11 0 0 0 0 0 0
Nucleus 0.51 0.53 0.62 0.31 0.27 0.25 0.22 0.22 0.21 0.17 0.23 0.23 0.55 0.43 0.28 0.38 0.38 0.26 0.25 0.23 0.2 0.11 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.19 0.25 0.14 0.32 0.17 0.18 0.18 0.16 0.19 0.16 0.12 0.11 0.15 0.18 0.21 0.5 0.45 0.5 0.32 0.38 0.33 0.36 0.25 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0.1 0.33 0.64 0.54 0.38 0.3 0.36 0.21 0.29 0.11 0.28 0.33 0.41 0.38 0.22 0.13 0.13 0.18 0.17 0.18 0.27 0.41 0.36
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0 0.08 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 1 0
Bud 0 2 1 4 5 5 13 15 14 20 13 20 0 0 0 0 0 0 1 2 1 0 2 6
Bud Neck 0 0 6 7 9 6 6 14 5 14 8 4 2 2 5 0 0 0 3 1 1 1 3 9
Bud Site 2 1 0 9 8 9 27 20 16 23 21 19 1 0 0 0 2 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 5 12 16 23 29 47 49 47 33 35 34 27 51 83 20 25 31 0 2 5 1 3 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 3 4 3 0 0 0 0 2 0
Endosome 3 7 2 6 29 36 25 8 6 4 2 6 7 20 29 21 16 17 19 17 13 15 33 48
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 1 2
Mitochondria 111 19 16 17 17 87 202 158 197 173 229 173 5 1 2 22 23 25 0 3 4 1 5 4
Nucleus 87 79 79 56 72 71 84 77 65 50 71 68 106 109 77 93 72 57 47 42 32 15 17 8
Nuclear Periphery 2 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 4 0 0 0 0 1 0 0
Nucleolus 32 37 18 58 45 52 66 59 57 47 38 34 29 46 57 125 85 112 60 70 53 49 65 65
Peroxisomes 0 0 0 3 2 4 4 0 1 0 0 0 0 0 3 1 0 1 0 0 0 0 0 1
SpindlePole 16 15 42 116 144 109 111 130 64 82 33 82 63 106 104 54 25 29 34 30 29 37 104 100
Vac/Vac Membrane 3 4 0 3 6 6 10 1 4 1 4 11 1 2 4 10 8 17 15 6 12 8 10 15
Unique Cell Count 171 148 128 181 267 287 375 358 306 286 305 297 192 256 276 248 188 222 190 184 164 139 259 278
Labelled Cell Count 261 169 176 295 360 414 595 531 479 448 458 451 243 339 364 349 264 295 190 184 164 139 259 278


Cytoplasm, Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 5.1 4.8 5.3 5.1 4.5 4.0 4.4 3.8 3.8 3.5 3.9 5.5 5.8 5.5 8.2 7.6 7.8 5.3 5.7 6.0
Std Deviation (1e-4) 0.5 1.0 0.8 2.3 1.4 1.8 1.6 1.5 1.7 1.2 1.3 1.1 0.9 1.4 1.6 2.0 2.2 2.3 1.2 1.6 1.6
Intensity Change (Log2) – – – 0.16 0.11 -0.1 -0.24 -0.11 -0.32 -0.31 -0.43 -0.27 0.2 0.29 0.21 0.79 0.67 0.72 0.17 0.27 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -0.4 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -0.2 0.2 1.0 1.3 1.7 0.7 0.6 0.6 1.3 2.6 4.6 -0.4 1.1 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.2 0 0 0 0 0 0 0 0 2.5 3.1 2.6 2.6 2.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.9 -2.1 0 0 0 0 0 0 0 -3.5 -5.4 -5.3 -1.1 -0.1 -0.3
Nucleus -5.4 -6.7 -7.2 -8.2 -8.4 -8.2 -9.0 -7.7 -7.7 -1.2 -3.5 -6.5 -4.5 -4.1 -6.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.6 0.7 0 0 0 0 0 0 0 0.3 1.0 1.6 6.9 5.8 6.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 5.4 3.9 0 0 0 0 0 0 0 0 1.6 0.9 -2.3 -4.2 -4.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6399 11.8112 10.1343 9.7879 9.7605 9.1738 7.9222 13.7661 12.2275 10.4644 10.1371 11.7335 10.0978 14.734 12.5738 11.3294 10.7523 12.4638
Actin 0.0402 0 0.0043 0.0083 0.0001 0.0106 0.0263 0.0001 0.0016 0.0019 0.0117 0.0001 0.016 0.0017 0.0057 0.013 0.0051 0.0033
Bud 0.001 0.0005 0.0006 0.0011 0.0002 0.0008 0.0022 0.0022 0.0027 0.0031 0.0031 0.0002 0.0009 0.0002 0.0031 0.0011 0.0003 0.0003
Bud Neck 0.005 0.0027 0.009 0.0073 0.0033 0.0048 0.0109 0.0031 0.0034 0.0075 0.0352 0.0051 0.0046 0.0006 0.0028 0.0036 0.004 0.0032
Bud Periphery 0.0012 0.0007 0.0009 0.0016 0.0004 0.0008 0.0015 0.001 0.0031 0.0041 0.0039 0.0003 0.001 0.0001 0.0012 0.0018 0.0006 0.0003
Bud Site 0.0069 0.0042 0.0033 0.0018 0.001 0.0007 0.0194 0.0131 0.0024 0.0065 0.0024 0.0008 0.0153 0.001 0.0025 0.0029 0.0009 0.0008
Cell Periphery 0.0009 0.0004 0.0005 0.0006 0.0004 0.0002 0.0007 0.0009 0.0007 0.0007 0.0005 0.0003 0.0009 0.0001 0.0007 0.0008 0.0002 0.0001
Cytoplasm 0.0291 0.0005 0.0063 0.001 0.0008 0.0063 0.0083 0.0081 0.0033 0.0091 0.0056 0.001 0.0046 0.0086 0.0027 0.009 0.0006 0.0014
Cytoplasmic Foci 0.0574 0.0006 0.005 0.0032 0.0011 0.0032 0.0322 0.0028 0.0049 0.0332 0.0304 0.004 0.0272 0.0033 0.0017 0.0188 0.0076 0.0032
Eisosomes 0.001 0 0.0001 0.0002 0 0.0003 0.0002 0 0 0 0.0001 0 0.0003 0 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0088 0.0001 0.0016 0.0007 0.0003 0.0054 0.0024 0.0003 0.0003 0.0015 0.0027 0.0002 0.0026 0 0.0002 0.0008 0.0002 0.0013
Endosome 0.0781 0.0005 0.0072 0.0114 0.0025 0.027 0.026 0.002 0.0057 0.0224 0.0219 0.0037 0.0284 0.0008 0.002 0.0082 0.0048 0.0142
Golgi 0.0205 0.0001 0.0015 0.0051 0.0003 0.0026 0.0063 0.0003 0.0046 0.0077 0.0038 0.0016 0.0084 0.0038 0.0017 0.0022 0.002 0.0064
Lipid Particles 0.0359 0.0006 0.0039 0.0165 0.0026 0.0063 0.0344 0.0027 0.0059 0.0237 0.0308 0.0087 0.0335 0.0003 0.0035 0.0284 0.0212 0.0014
Mitochondria 0.0106 0.0001 0.0163 0.0059 0.0003 0.0012 0.005 0.0002 0.0022 0.0092 0.0045 0.0089 0.0176 0.01 0.005 0.0032 0.13 0.0011
None 0.0693 0.0122 0.0008 0.0004 0.0001 0.0163 0.0198 0.0027 0.0156 0.0031 0.0021 0.0012 0.0141 0.0009 0.0168 0.0286 0.0004 0.0166
Nuclear Periphery 0.0364 0.0007 0.002 0.0046 0.008 0.0159 0.0134 0.0038 0.0017 0.0085 0.0146 0.0011 0.0031 0.0003 0.0003 0.008 0.0042 0.001
Nucleolus 0.3421 0.6475 0.6708 0.6633 0.7305 0.6339 0.5348 0.6547 0.6888 0.5735 0.5158 0.747 0.5639 0.6997 0.6964 0.5305 0.5759 0.6569
Nucleus 0.107 0.3044 0.2379 0.2511 0.2332 0.2282 0.1979 0.2773 0.2302 0.2035 0.2372 0.1763 0.217 0.254 0.2364 0.2692 0.1967 0.2578
Peroxisomes 0.0082 0.0001 0.0004 0.0021 0.0001 0.0005 0.0117 0.0003 0.001 0.0342 0.0072 0.0001 0.0054 0.0003 0.0033 0.0104 0.0064 0.0003
Punctate Nuclear 0.1118 0.019 0.0218 0.0069 0.01 0.0272 0.0405 0.0133 0.0128 0.0196 0.0565 0.0354 0.0272 0.0129 0.0095 0.0245 0.0364 0.0277
Vacuole 0.0226 0.0047 0.0046 0.0059 0.004 0.006 0.005 0.0106 0.0081 0.0225 0.0063 0.0033 0.0057 0.001 0.0041 0.0305 0.0015 0.0024
Vacuole Periphery 0.006 0.0003 0.001 0.0011 0.0009 0.0017 0.0012 0.0007 0.001 0.0043 0.0037 0.0008 0.0022 0.0002 0.0003 0.0043 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.4007 106.8315 77.7343 60.4585 86.4986 83.4923 61.0874 54.1474 41.0112 73.5903
Translational Efficiency 2.4108 2.1125 1.9503 2.1629 1.8824 1.9374 2.1315 1.6844 1.9072 1.8351

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘