Standard name
Description Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.11 0.30 0.21 0.14 0.19 0.23 0.28 0.41 0.34 0.16 0.42 0.46 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.08 0 0 0 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.10 0 0 0.06 0.05 0.06 0.05 0.06 0.07 0.09 0 0 0 0 0 0 0 0 0
Nucleus 0.18 0.22 0.11 0.07 0.14 0.19 0.18 0.13 0.09 0.07 0.09 0.08 0.05 0 0.07 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.86 0.80 0.87 0.67 0.71 0.81 0.78 0.75 0.77 0.70 0.66 0.84 0.65 0.64 0.60 0.90 0.95 0.83 0.86 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 1 0 1 0 1 0 0 0 1 2 0 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2 1 2 1 4 2 1 1 0 0 0 0 0 0 0
Cytoplasm 12 5 15 39 36 40 43 62 67 79 71 56 94 138 0 2 0 1 5 3
Endoplasmic Reticulum 1 0 2 3 2 3 0 2 0 1 1 1 3 0 0 0 0 1 0 2
Endosome 4 0 1 4 4 2 3 0 0 0 0 21 4 0 3 6 2 5 13 15
Golgi 0 0 1 1 0 0 0 0 1 0 0 1 0 1 1 0 0 2 1 2
Mitochondria 8 14 1 3 10 15 14 14 14 14 18 0 1 0 0 3 0 2 3 7
Nucleus 37 30 15 9 25 53 40 34 22 14 18 28 11 8 3 2 1 1 0 0
Nuclear Periphery 3 2 0 0 0 1 0 2 0 0 1 0 0 1 0 1 0 4 7 3
Nucleolus 3 1 1 1 3 2 1 0 0 0 0 1 0 0 2 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 0 0 0 5 0 0 1 0 0 0 1 2
Vac/Vac Membrane 178 111 117 86 124 225 177 203 186 137 138 290 147 191 29 198 134 149 254 173
Unique Cell Count 207 138 134 128 175 277 227 269 240 195 209 347 225 300 48 221 141 180 297 219
Labelled Cell Count 246 163 153 147 205 343 281 321 292 249 250 405 261 339 48 221 141 180 297 219

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Vacuole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.7 10.3 12.7 11.9 11.3 10.4 10.3 9.5 9.4 9.3 7.3 6.5 6.8 8.2 7.9 7.9
Std Deviation (1e-4) 2.3 2.7 2.9 4.2 4.4 4.6 4.3 4.0 3.7 3.6 3.8 1.5 1.7 2.0 2.1 1.8 1.7
Intensity Change (Log2) 0.2 0.5 0.4 0.4 0.3 0.2 0.1 0.1 0.1 -0.3 -0.4 -0.4 -0.1 -0.1 -0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.3 0 0 0 0 0 0 0 0 2.4 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 3.0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.4 -2.6 -1.5 -1.8 -1.4 -1.9 -1.5 -1.0 -0.5 0 -4.5 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.2 -2.8 -2.0 0.4 -1.4 -2.6 -1.8 -3.7 -4.8 1.1 -2.2 -3.2