Standard name
Description Cysteine desulfurase; involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.17 0.14 0 0 0.05 0.08 0.05 0.08
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.1 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0.67 0.78 0.62 0.06 0.12 0.07 0.12 0.1 0.08
Mitochondria 0.99 0.9 0.94 0.92 0.91 0.93 0.89 0.86 0.96 0.92 0.9 0.95 0.98 0.96 0.86 0 0.21 0.1 0.84 0.78 0.76 0.6 0.76 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.05 0.1 0.1 0.14 0.24 0.23 0.29 0.2 0.2 0.21 0.18 0 0.06 0.08 0.06 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.06 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.09 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.05 0.21 0.12 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 3 0 1 0 0 1 0 1 0 1 4 0 0 3 15 3 4 5 14 3 4 8
Bud 2 0 3 1 1 1 1 1 1 0 3 3 0 0 1 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 1 0 0 0 0 0 2 1 0 2 0 0 0 0 0 0
Cell Periphery 2 3 2 1 0 0 0 0 0 1 1 0 0 1 1 2 1 2 0 0 0 0 0 0
Cytoplasm 0 3 1 4 2 3 2 0 2 2 3 2 0 1 5 2 2 1 0 2 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 1 1 1 1 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0
Endosome 0 0 0 1 0 0 2 0 0 0 0 0 0 1 1 1 0 0 1 1 2 0 0 2
Golgi 0 19 12 4 4 1 1 3 1 0 1 1 8 3 6 32 67 13 9 25 20 4 10 9
Mitochondria 150 178 253 122 196 239 256 337 301 284 186 295 119 94 90 2 18 2 127 171 214 23 72 80
Nucleus 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 3 2 8 0 6 6 15 17 18 14 8 13 0 1 2 0 0 0 0 0 0 0 0 0
Nucleolus 25 9 26 13 30 61 67 112 62 63 43 56 2 6 8 3 2 0 3 1 1 1 3 0
Peroxisomes 1 0 7 3 1 0 6 2 5 4 4 2 0 0 0 0 1 1 3 2 19 2 1 6
SpindlePole 0 0 1 0 0 0 1 1 1 4 0 0 0 0 1 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 3 18 2 3 8 6 15 14 4 5 1 1 5 5 5 10 10 5 0 5 2 1 1 1
Unique Cell Count 152 198 268 132 215 256 287 390 315 308 207 312 122 98 105 48 86 21 151 220 280 39 96 112
Labelled Cell Count 187 237 320 152 249 318 368 490 398 380 251 376 138 112 120 56 118 27 151 220 280 39 96 112


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.3 29.7 27.2 26.9 29.7 29.4 27.1 26.7 25.2 23.0 24.3 23.8 24.7 24.7 24.2 27.9 33.0 34.3 19.9 24.0 25.7
Std Deviation (1e-4) 5.8 7.1 7.1 7.3 7.8 8.4 8.3 8.5 7.8 7.7 8.2 7.9 5.0 5.5 6.5 9.0 10.7 10.8 4.9 6.3 8.4
Intensity Change (Log2) -0.01 0.13 0.11 -0.01 -0.03 -0.11 -0.24 -0.16 -0.2 -0.14 -0.14 -0.17 0.04 0.28 0.34 -0.45 -0.18 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.7 -1.6 -3.0 -3.2 -3.1 -3.4 -3.8 -2.6 -3.4 0.9 0 0.5 11.5 14.2 0
Mitochondria -0.8 -1.4 -0.5 -2.2 -3.3 0.6 -1.0 -1.9 0.1 1.4 0.6 -2.8 -14.6 -14.0 -11.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -0.1 -0.5 1.3 0.9 1.6 1.0 0.5 0.8 0 0 0 0 0 0
Nucleolus 0 1.5 4.3 4.3 5.9 3.4 3.6 3.4 2.8 -2.9 -1.1 -0.6 0 -2.2 0
Peroxisomes 0 0 0 -0.4 0 -0.9 -1.1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 2.3 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nfs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available