Standard name | |
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Description | GTP/GDP exchange factor for Rsr1p (Bud1p); required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 37 | 50 | 20 | 31 | 28 | 47 | 25 | – | 14 | 13 | 16 | 12 | 45 | 39 | 18 | 3 | 0 | 2 | 35 | 74 | 40 | 19 | 26 | 7 |
Bud | 15 | 33 | 6 | 23 | 9 | 26 | 27 | – | 35 | 29 | 49 | 46 | 4 | 4 | 3 | 3 | 2 | 2 | 38 | 54 | 14 | 11 | 17 | 5 |
Bud Neck | 29 | 40 | 38 | 37 | 33 | 40 | 42 | – | 35 | 31 | 20 | 39 | 16 | 17 | 14 | 10 | 6 | 6 | 142 | 244 | 91 | 51 | 23 | 3 |
Bud Site | 55 | 65 | 7 | 17 | 6 | 31 | 34 | – | 23 | 34 | 53 | 51 | 5 | 8 | 6 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 3 | 2 | 0 | 1 | 2 | – | 0 | 3 | 2 | 3 | 2 | 1 | 1 | 2 | 5 | 4 | 1 | 2 | 2 | 3 | 9 | 5 |
Cytoplasm | 65 | 117 | 104 | 96 | 90 | 109 | 147 | – | 91 | 105 | 105 | 93 | 56 | 99 | 126 | 109 | 86 | 83 | 17 | 33 | 29 | 58 | 185 | 198 |
Endoplasmic Reticulum | 3 | 8 | 0 | 3 | 8 | 9 | 4 | – | 8 | 1 | 6 | 7 | 4 | 4 | 4 | 14 | 7 | 9 | 1 | 1 | 3 | 0 | 0 | 0 |
Endosome | 0 | 9 | 1 | 1 | 0 | 1 | 2 | – | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 5 | 1 | 13 | 12 | 23 | 5 | 2 | 3 | 2 |
Golgi | 0 | 1 | 1 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 5 | 3 | 3 | 0 | 0 | 2 | 0 |
Mitochondria | 40 | 14 | 0 | 3 | 4 | 24 | 43 | – | 76 | 68 | 202 | 133 | 0 | 1 | 0 | 9 | 4 | 2 | 11 | 21 | 23 | 4 | 14 | 5 |
Nucleus | 1 | 4 | 3 | 0 | 2 | 6 | 10 | – | 2 | 11 | 23 | 12 | 0 | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 22 | 66 | 2 | 6 | 2 | 16 | 14 | – | 17 | 12 | 30 | 26 | 1 | 9 | 18 | 7 | 11 | 17 | 5 | 4 | 5 | 0 | 0 | 0 |
SpindlePole | 52 | 91 | 47 | 50 | 29 | 50 | 78 | – | 31 | 48 | 34 | 81 | 31 | 44 | 69 | 22 | 22 | 39 | 7 | 18 | 4 | 0 | 2 | 2 |
Vac/Vac Membrane | 2 | 1 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 1 | 1 | 0 | 4 | 0 | 3 | 2 | 5 | 8 | 10 | 9 | 4 | 6 | 3 |
Unique Cell Count | 220 | 361 | 187 | 200 | 175 | 267 | 342 | 251 | 260 | 387 | 360 | 125 | 191 | 227 | 168 | 124 | 156 | 291 | 500 | 239 | 159 | 301 | 244 | |
Labelled Cell Count | 322 | 499 | 232 | 271 | 212 | 361 | 430 | 333 | 355 | 543 | 506 | 165 | 234 | 264 | 191 | 149 | 189 | 291 | 500 | 239 | 159 | 301 | 244 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 4.5 | 5.1 | 5.0 | 5.3 | 4.2 | 4.4 | – | 3.7 | 4.0 | 3.6 | 3.8 | 5.1 | 5.2 | 5.1 | 6.6 | 6.7 | 6.8 | 4.5 | 4.9 | 5.1 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.3 | 1.8 | 1.5 | 0.9 | 1.2 | – | 0.9 | 1.1 | 1.2 | 0.9 | 0.8 | 0.8 | 1.2 | 2.9 | 2.8 | 2.8 | 1.0 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.06 | -0.29 | -0.21 | – | -0.46 | -0.34 | -0.52 | -0.44 | 0.01 | 0.01 | -0.01 | 0.36 | 0.4 | 0.41 | -0.19 | -0.06 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0665 | -1.191 | -1.1906 | -1.3438 | -1.3631 | -0.9261 | -0.0001 | -0.0387 | 0.0941 | 0.2928 | 1.0214 | 0.1099 | -0.7301 | -0.6759 | -0.6149 | -0.454 | -1.1977 | -0.7672 |
Actin | 0.1243 | 0.0743 | 0.042 | 0.0527 | 0.0012 | 0.0333 | 0.0728 | 0.0219 | 0.031 | 0.0692 | 0.0477 | 0.0355 | 0.114 | 0.049 | 0.0264 | 0.0378 | 0.0059 | 0.0342 |
Bud | 0.0022 | 0.0038 | 0.0068 | 0.0007 | 0.0002 | 0.0005 | 0.0027 | 0.0036 | 0.0052 | 0.0014 | 0.0009 | 0.001 | 0.0038 | 0.0105 | 0.0052 | 0.0009 | 0.0083 | 0.0033 |
Bud Neck | 0.188 | 0.0772 | 0.381 | 0.815 | 0.9179 | 0.8614 | 0.2538 | 0.0502 | 0.3733 | 0.5895 | 0.6205 | 0.7962 | 0.2483 | 0.0331 | 0.4121 | 0.7725 | 0.7632 | 0.8378 |
Bud Periphery | 0.0048 | 0.0102 | 0.0206 | 0.0013 | 0.0003 | 0.0014 | 0.0075 | 0.0101 | 0.0185 | 0.0025 | 0.0026 | 0.005 | 0.0149 | 0.0088 | 0.0155 | 0.0026 | 0.0152 | 0.0028 |
Bud Site | 0.2469 | 0.4924 | 0.1557 | 0.0123 | 0.0195 | 0.0035 | 0.2254 | 0.4206 | 0.1756 | 0.033 | 0.0044 | 0.0055 | 0.3014 | 0.4626 | 0.2039 | 0.0448 | 0.0334 | 0.0055 |
Cell Periphery | 0.0092 | 0.0077 | 0.0071 | 0.0011 | 0.0005 | 0.0009 | 0.0103 | 0.0101 | 0.0084 | 0.0023 | 0.002 | 0.0016 | 0.0125 | 0.008 | 0.01 | 0.0059 | 0.0017 | 0.0017 |
Cytoplasm | 0.0406 | 0.0263 | 0.0497 | 0.0046 | 0.0009 | 0.0063 | 0.0348 | 0.042 | 0.0187 | 0.0063 | 0.0074 | 0.0089 | 0.0644 | 0.0953 | 0.067 | 0.0268 | 0.0056 | 0.0174 |
Cytoplasmic Foci | 0.053 | 0.0118 | 0.0195 | 0.0305 | 0.0243 | 0.0095 | 0.0261 | 0.0147 | 0.0237 | 0.0103 | 0.0203 | 0.0071 | 0.0241 | 0.0084 | 0.011 | 0.0091 | 0.0285 | 0.0073 |
Eisosomes | 0.0066 | 0.005 | 0.0039 | 0.002 | 0 | 0.0013 | 0.0049 | 0.0042 | 0.0029 | 0.0025 | 0.0008 | 0.0007 | 0.002 | 0.0014 | 0.0032 | 0.001 | 0.0004 | 0.0009 |
Endoplasmic Reticulum | 0.0017 | 0.0006 | 0.001 | 0.0004 | 0 | 0.0002 | 0.0024 | 0.001 | 0.0008 | 0.0003 | 0.0131 | 0.0007 | 0.0026 | 0.0008 | 0.002 | 0.0009 | 0.0001 | 0.0001 |
Endosome | 0.0133 | 0.0004 | 0.0069 | 0.0007 | 0.003 | 0.0031 | 0.0107 | 0.0019 | 0.0045 | 0.0023 | 0.0098 | 0.0124 | 0.0087 | 0.0004 | 0.0024 | 0.0076 | 0.0023 | 0.0002 |
Golgi | 0.0104 | 0.0006 | 0.0085 | 0.0008 | 0.0017 | 0.0059 | 0.0035 | 0.001 | 0.0037 | 0.0041 | 0.005 | 0.0065 | 0.0044 | 0.0005 | 0.0015 | 0.0065 | 0.0022 | 0.0003 |
Lipid Particles | 0.0204 | 0.0008 | 0.0048 | 0.0042 | 0.0161 | 0.0063 | 0.0125 | 0.0006 | 0.0087 | 0.0042 | 0.0126 | 0.0053 | 0.0115 | 0.0005 | 0.0014 | 0.015 | 0.0121 | 0.0001 |
Mitochondria | 0.0064 | 0.0013 | 0.0071 | 0.0005 | 0.0007 | 0.0151 | 0.0028 | 0.0014 | 0.0043 | 0.0013 | 0.0874 | 0.0021 | 0.0019 | 0.0005 | 0.0011 | 0.0009 | 0.0004 | 0.0006 |
None | 0.2221 | 0.2792 | 0.2603 | 0.0608 | 0.0005 | 0.0289 | 0.298 | 0.407 | 0.2906 | 0.2564 | 0.0487 | 0.0928 | 0.1586 | 0.3099 | 0.2247 | 0.042 | 0.0672 | 0.0843 |
Nuclear Periphery | 0.001 | 0.0006 | 0.0006 | 0.0003 | 0 | 0.0003 | 0.0019 | 0.0003 | 0.0015 | 0.0002 | 0.0261 | 0.0021 | 0.0035 | 0.0003 | 0.001 | 0.0038 | 0.0007 | 0.0001 |
Nucleolus | 0.0038 | 0.0012 | 0.0024 | 0.001 | 0.0019 | 0.0101 | 0.0027 | 0.0017 | 0.0032 | 0.0015 | 0.0008 | 0.0007 | 0.0017 | 0.0012 | 0.002 | 0.0016 | 0.0006 | 0.0005 |
Nucleus | 0.0074 | 0.001 | 0.0028 | 0.0007 | 0.0003 | 0.0015 | 0.0029 | 0.0016 | 0.0026 | 0.0015 | 0.0108 | 0.0011 | 0.0025 | 0.0016 | 0.003 | 0.0013 | 0.0008 | 0.0009 |
Peroxisomes | 0.0227 | 0.0031 | 0.0106 | 0.0022 | 0.0086 | 0.0072 | 0.0093 | 0.0013 | 0.0099 | 0.0089 | 0.0737 | 0.0082 | 0.0066 | 0.0014 | 0.0012 | 0.0043 | 0.027 | 0.0005 |
Punctate Nuclear | 0.0113 | 0.0016 | 0.0056 | 0.0077 | 0.0017 | 0.0028 | 0.012 | 0.003 | 0.0105 | 0.002 | 0.0018 | 0.0049 | 0.0098 | 0.0045 | 0.0029 | 0.0138 | 0.0235 | 0.0012 |
Vacuole | 0.0036 | 0.0007 | 0.0025 | 0.0002 | 0.0006 | 0.0003 | 0.0024 | 0.0016 | 0.0021 | 0.0003 | 0.0018 | 0.0011 | 0.0023 | 0.0013 | 0.0024 | 0.0005 | 0.0008 | 0.0003 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0017 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.4321 | 5.1134 | 4.1738 | 5.6529 | 2.5957 | 4.0018 | 7.109 | 6.2811 | 5.0091 | 6.2038 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |