Standard name
Description GTP/GDP exchange factor for Rsr1p (Bud1p); required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.17 0.14 0.11 0.15 0.16 0.18 0.07 – 0.06 0.05 0 0 0.36 0.2 0.08 0 0 0 0.12 0.15 0.17 0.12 0.09 0
Bud 0.07 0.09 0 0.12 0.05 0.1 0.08 – 0.14 0.11 0.13 0.13 0 0 0 0 0 0 0.13 0.11 0.06 0.07 0.06 0
Bud Neck 0.13 0.11 0.2 0.18 0.19 0.15 0.12 – 0.14 0.12 0.05 0.11 0.13 0.09 0.06 0.06 0.05 0 0.49 0.49 0.38 0.32 0.08 0
Bud Site 0.25 0.18 0 0.09 0 0.12 0.1 – 0.09 0.13 0.14 0.14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.32 0.56 0.48 0.51 0.41 0.43 – 0.36 0.4 0.27 0.26 0.45 0.52 0.56 0.65 0.69 0.53 0.06 0.07 0.13 0.37 0.62 0.81
Endoplasmic Reticulum 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0 0 0 0.09 0.13 – 0.3 0.26 0.52 0.37 0 0 0 0.05 0 0 0 0 0.1 0 0.05 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0.18 0 0 0 0.06 0 – 0.07 0.05 0.08 0.07 0 0.05 0.08 0 0.09 0.11 0 0 0 0 0 0
SpindlePole 0.24 0.25 0.25 0.25 0.17 0.19 0.23 – 0.12 0.18 0.09 0.23 0.25 0.23 0.3 0.13 0.18 0.25 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 37 50 20 31 28 47 25 – 14 13 16 12 45 39 18 3 0 2 35 74 40 19 26 7
Bud 15 33 6 23 9 26 27 – 35 29 49 46 4 4 3 3 2 2 38 54 14 11 17 5
Bud Neck 29 40 38 37 33 40 42 – 35 31 20 39 16 17 14 10 6 6 142 244 91 51 23 3
Bud Site 55 65 7 17 6 31 34 – 23 34 53 51 5 8 6 0 1 1 – – – – – –
Cell Periphery 1 0 3 2 0 1 2 – 0 3 2 3 2 1 1 2 5 4 1 2 2 3 9 5
Cytoplasm 65 117 104 96 90 109 147 – 91 105 105 93 56 99 126 109 86 83 17 33 29 58 185 198
Endoplasmic Reticulum 3 8 0 3 8 9 4 – 8 1 6 7 4 4 4 14 7 9 1 1 3 0 0 0
Endosome 0 9 1 1 0 1 2 – 0 0 0 2 1 2 1 5 1 13 12 23 5 2 3 2
Golgi 0 1 1 2 0 0 0 – 0 0 0 0 0 0 0 3 1 5 3 3 0 0 2 0
Mitochondria 40 14 0 3 4 24 43 – 76 68 202 133 0 1 0 9 4 2 11 21 23 4 14 5
Nucleus 1 4 3 0 2 6 10 – 2 11 23 12 0 2 3 1 0 1 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 – 0 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0
Peroxisomes 22 66 2 6 2 16 14 – 17 12 30 26 1 9 18 7 11 17 5 4 5 0 0 0
SpindlePole 52 91 47 50 29 50 78 – 31 48 34 81 31 44 69 22 22 39 7 18 4 0 2 2
Vac/Vac Membrane 2 1 0 0 1 1 0 – 1 0 1 1 0 4 0 3 2 5 8 10 9 4 6 3
Unique Cell Count 220 361 187 200 175 267 342 251 260 387 360 125 191 227 168 124 156 291 500 239 159 301 244
Labelled Cell Count 322 499 232 271 212 361 430 333 355 543 506 165 234 264 191 149 189 291 500 239 159 301 244


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.5 5.1 5.0 5.3 4.2 4.4 – 3.7 4.0 3.6 3.8 5.1 5.2 5.1 6.6 6.7 6.8 4.5 4.9 5.1
Std Deviation (1e-4) 0.8 0.7 1.3 1.8 1.5 0.9 1.2 – 0.9 1.1 1.2 0.9 0.8 0.8 1.2 2.9 2.8 2.8 1.0 1.2 1.1
Intensity Change (Log2) – – – -0.03 0.06 -0.29 -0.21 – -0.46 -0.34 -0.52 -0.44 0.01 0.01 -0.01 0.36 0.4 0.41 -0.19 -0.06 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0665 -1.191 -1.1906 -1.3438 -1.3631 -0.9261 -0.0001 -0.0387 0.0941 0.2928 1.0214 0.1099 -0.7301 -0.6759 -0.6149 -0.454 -1.1977 -0.7672
Actin 0.1243 0.0743 0.042 0.0527 0.0012 0.0333 0.0728 0.0219 0.031 0.0692 0.0477 0.0355 0.114 0.049 0.0264 0.0378 0.0059 0.0342
Bud 0.0022 0.0038 0.0068 0.0007 0.0002 0.0005 0.0027 0.0036 0.0052 0.0014 0.0009 0.001 0.0038 0.0105 0.0052 0.0009 0.0083 0.0033
Bud Neck 0.188 0.0772 0.381 0.815 0.9179 0.8614 0.2538 0.0502 0.3733 0.5895 0.6205 0.7962 0.2483 0.0331 0.4121 0.7725 0.7632 0.8378
Bud Periphery 0.0048 0.0102 0.0206 0.0013 0.0003 0.0014 0.0075 0.0101 0.0185 0.0025 0.0026 0.005 0.0149 0.0088 0.0155 0.0026 0.0152 0.0028
Bud Site 0.2469 0.4924 0.1557 0.0123 0.0195 0.0035 0.2254 0.4206 0.1756 0.033 0.0044 0.0055 0.3014 0.4626 0.2039 0.0448 0.0334 0.0055
Cell Periphery 0.0092 0.0077 0.0071 0.0011 0.0005 0.0009 0.0103 0.0101 0.0084 0.0023 0.002 0.0016 0.0125 0.008 0.01 0.0059 0.0017 0.0017
Cytoplasm 0.0406 0.0263 0.0497 0.0046 0.0009 0.0063 0.0348 0.042 0.0187 0.0063 0.0074 0.0089 0.0644 0.0953 0.067 0.0268 0.0056 0.0174
Cytoplasmic Foci 0.053 0.0118 0.0195 0.0305 0.0243 0.0095 0.0261 0.0147 0.0237 0.0103 0.0203 0.0071 0.0241 0.0084 0.011 0.0091 0.0285 0.0073
Eisosomes 0.0066 0.005 0.0039 0.002 0 0.0013 0.0049 0.0042 0.0029 0.0025 0.0008 0.0007 0.002 0.0014 0.0032 0.001 0.0004 0.0009
Endoplasmic Reticulum 0.0017 0.0006 0.001 0.0004 0 0.0002 0.0024 0.001 0.0008 0.0003 0.0131 0.0007 0.0026 0.0008 0.002 0.0009 0.0001 0.0001
Endosome 0.0133 0.0004 0.0069 0.0007 0.003 0.0031 0.0107 0.0019 0.0045 0.0023 0.0098 0.0124 0.0087 0.0004 0.0024 0.0076 0.0023 0.0002
Golgi 0.0104 0.0006 0.0085 0.0008 0.0017 0.0059 0.0035 0.001 0.0037 0.0041 0.005 0.0065 0.0044 0.0005 0.0015 0.0065 0.0022 0.0003
Lipid Particles 0.0204 0.0008 0.0048 0.0042 0.0161 0.0063 0.0125 0.0006 0.0087 0.0042 0.0126 0.0053 0.0115 0.0005 0.0014 0.015 0.0121 0.0001
Mitochondria 0.0064 0.0013 0.0071 0.0005 0.0007 0.0151 0.0028 0.0014 0.0043 0.0013 0.0874 0.0021 0.0019 0.0005 0.0011 0.0009 0.0004 0.0006
None 0.2221 0.2792 0.2603 0.0608 0.0005 0.0289 0.298 0.407 0.2906 0.2564 0.0487 0.0928 0.1586 0.3099 0.2247 0.042 0.0672 0.0843
Nuclear Periphery 0.001 0.0006 0.0006 0.0003 0 0.0003 0.0019 0.0003 0.0015 0.0002 0.0261 0.0021 0.0035 0.0003 0.001 0.0038 0.0007 0.0001
Nucleolus 0.0038 0.0012 0.0024 0.001 0.0019 0.0101 0.0027 0.0017 0.0032 0.0015 0.0008 0.0007 0.0017 0.0012 0.002 0.0016 0.0006 0.0005
Nucleus 0.0074 0.001 0.0028 0.0007 0.0003 0.0015 0.0029 0.0016 0.0026 0.0015 0.0108 0.0011 0.0025 0.0016 0.003 0.0013 0.0008 0.0009
Peroxisomes 0.0227 0.0031 0.0106 0.0022 0.0086 0.0072 0.0093 0.0013 0.0099 0.0089 0.0737 0.0082 0.0066 0.0014 0.0012 0.0043 0.027 0.0005
Punctate Nuclear 0.0113 0.0016 0.0056 0.0077 0.0017 0.0028 0.012 0.003 0.0105 0.002 0.0018 0.0049 0.0098 0.0045 0.0029 0.0138 0.0235 0.0012
Vacuole 0.0036 0.0007 0.0025 0.0002 0.0006 0.0003 0.0024 0.0016 0.0021 0.0003 0.0018 0.0011 0.0023 0.0013 0.0024 0.0005 0.0008 0.0003
Vacuole Periphery 0.0004 0.0001 0.0006 0.0001 0.0001 0.0003 0.0005 0.0001 0.0004 0.0001 0.0017 0.0006 0.0004 0.0001 0.0002 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.4321 5.1134 4.1738 5.6529 2.5957 4.0018 7.109 6.2811 5.0091 6.2038
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –