Standard name
Human Ortholog
Description Mitochondrial type 2C protein phosphatase (PP2C); has similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0.07 0.05 0.05 0.06 0.05 0.07 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.39 0.21 0.2 0.13 0.05 0 0 0 0 0 0 0.69 0.68 0.74 0.23 0.16 0.16 0 0.07 0 0.12 0.09 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0 0.07 0 0 0 0 0 0 0 0.08 0.09 0.1 0.25 0.29 0.44 0.13 0.09 0.15 0.06 0.07 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.74 0.53 0.54 0.64 0.71 0.91 0.92 0.85 0.9 0.94 0.94 0.94 0 0 0 0.23 0.08 0.06 0.59 0.63 0.57 0.61 0.55 0.54
Nucleus 0 0 0 0 0 0 0.06 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.06 0.11 0.05 0 0 0 0 0 0 0 0 0.09 0 0.17 0.21 0.28 0 0 0.08 0 0 0
SpindlePole 0.11 0.11 0.16 0.15 0.13 0.07 0.05 0.11 0.07 0 0 0 0.14 0.12 0.11 0.23 0.3 0.22 0.08 0.07 0.07 0 0.09 0.09
Vac/Vac Membrane 0 0.08 0.05 0 0.08 0.05 0 0 0.05 0 0 0 0 0.07 0 0.12 0.13 0.12 0.05 0.06 0 0.06 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 1 0 2 0 1 0 0 0 0 0 5 4 0 0 0 0 3 1 13 7 8 17
Bud 11 3 23 31 27 29 64 46 51 48 28 45 5 5 6 0 0 1 2 0 3 1 11 13
Bud Neck 0 1 3 3 7 0 2 3 1 0 0 1 7 3 6 0 1 0 2 1 2 4 5 9
Bud Site 0 1 6 7 4 17 23 19 17 23 20 17 2 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 5 0 0 5 1 3 2 2 2 0 7 4 1 2 0 0 0 1 1 1 3
Cytoplasm 46 58 91 83 75 31 26 32 21 20 24 11 143 202 317 9 12 5 10 13 8 31 37 36
Endoplasmic Reticulum 1 4 0 3 3 3 2 2 0 0 0 0 2 1 3 2 0 0 4 1 6 1 5 1
Endosome 8 6 35 10 37 14 1 16 2 3 2 2 17 27 44 10 22 14 39 18 81 15 31 40
Golgi 0 0 2 2 1 0 0 0 0 0 0 0 0 0 1 0 0 1 3 1 6 0 5 5
Mitochondria 189 80 230 266 399 522 926 721 670 856 547 830 9 5 5 9 6 2 183 121 310 163 241 300
Nucleus 0 1 0 0 5 8 61 48 48 52 22 28 0 0 1 0 0 0 0 0 0 2 2 3
Nuclear Periphery 0 0 1 0 1 2 25 25 34 28 24 34 0 0 0 1 0 0 0 0 5 1 1 2
Nucleolus 1 0 2 0 1 7 41 37 30 47 15 33 0 0 0 0 0 0 1 1 1 1 1 2
Peroxisomes 12 3 25 45 31 15 9 21 14 9 5 14 3 28 11 7 16 9 13 2 42 3 9 19
SpindlePole 28 17 67 62 72 40 54 92 53 38 19 39 29 35 49 9 23 7 24 14 39 11 40 51
Vac/Vac Membrane 11 12 21 10 44 31 39 25 35 11 12 33 9 20 18 5 10 4 14 11 6 16 26 35
Unique Cell Count 256 150 424 417 565 574 1012 851 743 913 582 884 207 298 428 40 76 32 313 194 543 268 440 557
Labelled Cell Count 308 190 509 527 709 719 1279 1088 979 1137 720 1089 231 337 465 53 92 43 313 194 543 268 440 557


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.3 3.8 3.8 4.0 3.6 3.6 3.7 3.6 3.3 3.1 3.2 5.5 5.7 5.3 5.6 5.9 6.6 5.5 5.8 5.7
Std Deviation (1e-4) 0.6 0.8 0.8 1.4 1.2 1.2 1.1 1.2 1.3 1.0 1.1 0.8 1.1 2.4 1.2 1.5 1.5 2.4 1.2 1.7 1.2
Intensity Change (Log2) – – – -0.0 0.08 -0.07 -0.05 -0.04 -0.07 -0.18 -0.28 -0.22 0.54 0.59 0.49 0.56 0.65 0.8 0.54 0.63 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5509 4.1642 3.9923 3.6318 3.4622 3.9706 0.5465 0.4935 0.187 0.2802 -0.2687 0.1587 -1.323 -0.4576 -0.7924 -0.7975 -1.2618 -0.7948
Actin 0.0086 0.007 0.0083 0.0042 0.0115 0.0071 0.0528 0.0158 0.036 0.0086 0.0071 0.0115 0.023 0.0178 0.0115 0.0039 0.0046 0.0141
Bud 0.0022 0.0031 0.0028 0.0022 0.0018 0.0007 0.0014 0.0041 0.0042 0.0054 0.002 0.0016 0.0018 0.0029 0.0022 0.0014 0.003 0.0008
Bud Neck 0.0018 0.0025 0.0112 0.0076 0.0039 0.0036 0.0068 0.0022 0.0067 0.0059 0.0018 0.006 0.0029 0.0029 0.004 0.002 0.0036 0.0082
Bud Periphery 0.008 0.0076 0.0109 0.0124 0.0055 0.0028 0.0022 0.0068 0.0096 0.0199 0.0037 0.0032 0.0026 0.0039 0.0038 0.003 0.0055 0.0017
Bud Site 0.0099 0.0508 0.0156 0.0046 0.0035 0.0015 0.0096 0.0423 0.0188 0.0106 0.002 0.0018 0.0066 0.0378 0.0065 0.0012 0.0019 0.0014
Cell Periphery 0.0038 0.0026 0.003 0.0176 0.0013 0.0025 0.0009 0.002 0.0024 0.0008 0.0004 0.0005 0.0008 0.001 0.0007 0.0003 0.0005 0.0006
Cytoplasm 0.0405 0.0948 0.0502 0.0316 0.0484 0.0697 0.0437 0.1158 0.0797 0.0597 0.0332 0.0748 0.0224 0.065 0.0552 0.0275 0.0293 0.0441
Cytoplasmic Foci 0.1121 0.1179 0.08 0.0832 0.1396 0.104 0.1334 0.1475 0.1193 0.1341 0.1313 0.1112 0.1247 0.1282 0.0995 0.0993 0.0847 0.1262
Eisosomes 0.0018 0.0011 0.0009 0.0006 0.0005 0.0008 0.0012 0.0007 0.0009 0.0004 0.0001 0.0002 0.0006 0.0005 0.0005 0.0002 0.0001 0.0003
Endoplasmic Reticulum 0.0046 0.0036 0.0028 0.0012 0.0018 0.0043 0.0102 0.0068 0.0046 0.0021 0.0027 0.006 0.005 0.0081 0.0036 0.001 0.001 0.0023
Endosome 0.1392 0.1234 0.0965 0.0531 0.144 0.1147 0.2166 0.1767 0.1612 0.1909 0.1868 0.1869 0.2033 0.1923 0.1382 0.1812 0.1104 0.17
Golgi 0.0436 0.0374 0.033 0.0196 0.0549 0.0464 0.0653 0.0698 0.0655 0.0595 0.1212 0.0986 0.0925 0.1014 0.0778 0.0615 0.067 0.0906
Lipid Particles 0.0467 0.0198 0.0232 0.0034 0.026 0.0417 0.0849 0.02 0.0158 0.0121 0.0283 0.0214 0.0784 0.0138 0.0176 0.0096 0.008 0.0279
Mitochondria 0.3425 0.3246 0.4635 0.5879 0.3062 0.3787 0.1861 0.2682 0.3306 0.4303 0.4212 0.369 0.3036 0.3002 0.4483 0.5377 0.6177 0.4295
None 0.1421 0.1342 0.1383 0.1224 0.1316 0.145 0.0743 0.0586 0.0921 0.0321 0.0021 0.0346 0.0482 0.0343 0.0705 0.0211 0.003 0.0242
Nuclear Periphery 0.0041 0.0014 0.001 0.0007 0.0006 0.001 0.0168 0.003 0.0021 0.0004 0.0007 0.0053 0.0028 0.0019 0.0023 0.0007 0.0002 0.0011
Nucleolus 0.0028 0.002 0.0026 0.0012 0.0007 0.0012 0.0018 0.0023 0.0015 0.0003 0.0007 0.0004 0.0011 0.0007 0.0008 0.0005 0.0004 0.0004
Nucleus 0.0033 0.0023 0.0019 0.0015 0.001 0.0013 0.0035 0.0029 0.0021 0.0007 0.0008 0.0014 0.0009 0.0011 0.0013 0.0014 0.0005 0.0008
Peroxisomes 0.0553 0.0427 0.0377 0.0309 0.1024 0.0575 0.0501 0.0317 0.0254 0.016 0.0397 0.0471 0.0629 0.0646 0.0415 0.0319 0.0506 0.0447
Punctate Nuclear 0.0116 0.0013 0.0012 0.0011 0.0008 0.0014 0.0132 0.0019 0.0029 0.0006 0.0005 0.0021 0.0019 0.0014 0.0019 0.0008 0.0002 0.0016
Vacuole 0.0113 0.0159 0.0108 0.0088 0.0103 0.01 0.0173 0.0156 0.0123 0.0061 0.0079 0.0085 0.0093 0.0132 0.008 0.0085 0.0043 0.0052
Vacuole Periphery 0.0042 0.0039 0.0049 0.0043 0.0038 0.0042 0.0077 0.0054 0.0061 0.0032 0.0059 0.0081 0.0045 0.0071 0.0045 0.0053 0.0034 0.0044

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3781 14.1017 6.6089 11.2632 12.9175 12.4469 12.1945 20.0064 19.1678 12.1386
Translational Efficiency 0.35 0.2163 0.6395 0.4938 0.281 0.3781 0.389 0.2455 0.3318 0.4524

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘