Standard name | |
---|---|
Description | Substrate-specific adaptor protein involved in apo-Rli1p maturation; subunit of the Yae1-Lto1 complex, recruiting apo-Rli1p to the CIA targeting complex, facilitating the insertion of an Fe/S cluster by the Fe-S biosynthetic machinery; deca-GX3 motif crucial for complex formation; essential for aerobic but not anaerobic growth; homolog of human YAE1D1, which is overexpressed in oral cancers; co-expression of YAE1D1 and LTO1 homolog ORAOV1 restores growth and Rli1p maturation after YAE1 depletion |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 3 | 7 | 0 | 0 | 1 | – | 6 | 1 | 7 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 0 | 1 | 0 | 1 | – | 4 | 1 | 1 | 4 | 11 | 1 | 3 | 0 | 11 | 8 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 445 | 456 | 204 | 266 | 299 | 249 | – | 283 | 229 | 271 | 192 | 268 | 206 | 226 | 244 | 194 | 143 | 141 | 396 | 439 | 211 | 128 | 186 | 188 |
Endoplasmic Reticulum | 3 | 2 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 5 | 2 | 4 | 1 | 0 | 1 | 0 | 3 | 2 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 1 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 2 | 0 | 0 | 0 | 1 |
Mitochondria | 7 | 2 | 6 | 44 | 22 | 85 | – | 141 | 146 | 192 | 139 | 200 | 1 | 0 | 0 | 1 | 5 | 2 | 4 | 3 | 1 | 3 | 2 | 0 |
Nucleus | 37 | 4 | 0 | 2 | 5 | 3 | – | 3 | 1 | 4 | 4 | 5 | 1 | 0 | 0 | 2 | 4 | 3 | 8 | 2 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 0 | 1 | – | 0 | 4 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 0 | 6 | 6 | 4 | – | 11 | 9 | 5 | 2 | 5 | 0 | 0 | 2 | 11 | 10 | 5 | 83 | 3 | 2 | 1 | 0 | 1 |
Unique Cell Count | 473 | 464 | 209 | 279 | 320 | 278 | 355 | 300 | 382 | 277 | 383 | 207 | 228 | 246 | 203 | 159 | 148 | 511 | 459 | 224 | 140 | 204 | 203 | |
Labelled Cell Count | 494 | 474 | 220 | 319 | 335 | 345 | 448 | 392 | 483 | 349 | 495 | 210 | 231 | 247 | 225 | 175 | 163 | 511 | 459 | 224 | 140 | 204 | 203 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 8.0 | 4.8 | 5.1 | 5.3 | 4.3 | – | 4.0 | 3.6 | 3.7 | 3.6 | 3.4 | 7.5 | 7.6 | 7.5 | 11.9 | 11.4 | 11.3 | 7.8 | 8.5 | 8.2 |
Std Deviation (1e-4) | 1.2 | 1.1 | 1.2 | 1.5 | 1.4 | 1.6 | – | 0.8 | 0.9 | 0.8 | 1.2 | 0.5 | 1.4 | 1.1 | 1.5 | 2.0 | 1.9 | 2.0 | 2.1 | 2.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.15 | -0.17 | – | -0.29 | -0.43 | -0.38 | -0.41 | -0.5 | 0.64 | 0.66 | 0.64 | 1.3 | 1.24 | 1.22 | 0.7 | 0.82 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 |
Cytoplasm | -1.3 | -2.2 | -3.4 | – | -6.0 | -6.6 | -7.8 | -7.9 | -8.0 | 1.6 | 1.3 | 1.4 | -1.1 | -3.1 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.6 | 2.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 |