Standard name
Description Substrate-specific adaptor protein involved in apo-Rli1p maturation; subunit of the Yae1-Lto1 complex, recruiting apo-Rli1p to the CIA targeting complex, facilitating the insertion of an Fe/S cluster by the Fe-S biosynthetic machinery; deca-GX3 motif crucial for complex formation; essential for aerobic but not anaerobic growth; homolog of human YAE1D1, which is overexpressed in oral cancers; co-expression of YAE1D1 and LTO1 homolog ORAOV1 restores growth and Rli1p maturation after YAE1 depletion
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.98 0.95 0.93 0.9 – 0.8 0.76 0.71 0.69 0.7 1.0 0.99 0.99 0.96 0.9 0.95 0.78 0.96 0.94 0.92 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.16 0.07 0.31 – 0.4 0.49 0.5 0.5 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.06 0 0.16 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Bud 0 3 7 0 0 1 – 6 1 7 4 2 1 0 0 0 0 0 1 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 1 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 0 1 0 1 – 4 1 1 4 11 1 3 0 11 8 8 0 0 0 0 0 0
Cytoplasm 445 456 204 266 299 249 – 283 229 271 192 268 206 226 244 194 143 141 396 439 211 128 186 188
Endoplasmic Reticulum 3 2 2 0 0 0 – 0 0 0 1 2 0 1 0 5 2 4 1 0 1 0 3 2
Endosome 0 0 0 0 2 0 – 0 0 1 0 0 0 1 0 0 0 0 4 1 1 1 0 1
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 3 0 3 2 0 0 0 1
Mitochondria 7 2 6 44 22 85 – 141 146 192 139 200 1 0 0 1 5 2 4 3 1 3 2 0
Nucleus 37 4 0 2 5 3 – 3 1 4 4 5 1 0 0 2 4 3 8 2 0 1 1 0
Nuclear Periphery 1 2 0 0 0 1 – 0 4 1 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 1 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 6 6 4 – 11 9 5 2 5 0 0 2 11 10 5 83 3 2 1 0 1
Unique Cell Count 473 464 209 279 320 278 355 300 382 277 383 207 228 246 203 159 148 511 459 224 140 204 203
Labelled Cell Count 494 474 220 319 335 345 448 392 483 349 495 210 231 247 225 175 163 511 459 224 140 204 203


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 8.0 4.8 5.1 5.3 4.3 – 4.0 3.6 3.7 3.6 3.4 7.5 7.6 7.5 11.9 11.4 11.3 7.8 8.5 8.2
Std Deviation (1e-4) 1.2 1.1 1.2 1.5 1.4 1.6 – 0.8 0.9 0.8 1.2 0.5 1.4 1.1 1.5 2.0 1.9 2.0 2.1 2.2 1.5
Intensity Change (Log2) – – – 0.06 0.15 -0.17 – -0.29 -0.43 -0.38 -0.41 -0.5 0.64 0.66 0.64 1.3 1.24 1.22 0.7 0.82 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 3.4 0 0
Cytoplasm -1.3 -2.2 -3.4 – -6.0 -6.6 -7.8 -7.9 -8.0 1.6 1.3 1.4 -1.1 -3.1 -1.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.6 2.0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 3.4 0 0