Standard name
Description Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0.06 0.1 0.12 0.13 0.1 0.15 0.1 0 0 0 0.06 0.05 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.09 0.08 0.15 0.43 0.32 0.38 0.42 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.95 0.93 0.89 0.88 0.9 0.86 0.78 0.8 0.84 0.77 0.88 0.85 0.84 0.78 0.81 0.78 0.94 0.93 0.92 0.78 0.71 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.13 0 0 0 0.07 0.13 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 1 0 6 0 7 8 6 0 0 0 0 0 0 2 2 1 4 7 9
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 5 0 0 0 0 0 0 0 0 2 0 1 0
Bud Site 0 0 0 0 7 19 2 9 18 13 12 17 0 0 1 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 6 16 17 18 20 19 20 22 18 15 33 20 36 43 12 11 6 0 2 1 18 17 27
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 2 0 0 0 2 2 3
Endosome 0 0 0 0 0 2 0 1 0 1 1 0 2 1 0 1 0 0 1 4 1 2 5 7
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 1
Mitochondria 0 8 0 0 28 39 40 73 216 168 165 232 1 3 1 7 3 2 0 2 1 2 2 7
Nucleus 180 291 392 260 278 372 453 421 387 422 365 419 181 245 281 92 61 49 184 296 398 237 272 230
Nuclear Periphery 0 0 2 1 3 1 9 4 6 3 3 1 0 0 1 2 0 0 0 0 0 0 1 3
Nucleolus 2 1 0 1 11 12 11 7 23 20 23 16 2 4 12 3 1 2 0 0 2 4 11 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 3 7 4 3 3 7 0 0 2 0 0 0 0 0 1 2 4 5
Vac/Vac Membrane 9 8 9 1 3 8 6 16 8 11 13 10 6 6 4 9 2 8 3 7 15 21 51 85
Unique Cell Count 189 308 411 279 312 421 502 488 497 527 437 546 206 289 333 118 75 63 195 318 432 305 384 408
Labelled Cell Count 196 314 419 280 349 475 543 565 684 666 608 746 213 297 345 130 79 69 195 318 432 305 384 408


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 8.1 9.8 9.2 6.3 6.2 6.0 5.5 4.5 5.0 4.6 4.4 9.8 8.6 8.9 9.1 11.6 11.0 10.5 10.7 10.1
Std Deviation (1e-4) 3.1 1.6 2.5 3.0 1.9 1.9 1.5 1.4 1.3 1.3 1.3 0.9 2.7 2.6 2.7 2.6 2.8 3.5 2.7 3.1 3.1
Intensity Change (Log2) -0.08 -0.64 -0.65 -0.72 -0.83 -1.11 -0.98 -1.08 -1.15 0.0 -0.19 -0.14 -0.11 0.24 0.17 0.09 0.12 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 4.4 0 0 0 3.2 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.2 0.6 -0.1 0.2 0.4 -0.4 -0.4 1.5 2.9 4.3 4.5 2.7 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 6.2 6.3 5.9 8.2 0 12.6 0 0 0 0 0 0 0 0
Nucleus -1.2 -3.2 -3.7 -2.9 -4.6 -7.5 -6.9 -5.6 -7.9 -3.4 -4.8 -5.1 -6.0 -4.4 -5.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.4 0 0 0 4.0 0 0 0 0 3.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -1.3 -0.3 -1.2 1.0 -0.6 -0.1 0.7 -0.4 0.5 -0.1 -1.0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available