Standard name
Description Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.96 0.95 0.91 0.91 0.91 0.84 0.82 0.76 0.83 0.99 0.98 0.97 0.94 0.97 0.95 0.97 0.97 0.95 0.88 0.89 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.12 0.05 0.28 0.28 0.33 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 3 3
Bud 0 0 4 0 0 2 3 3 4 9 9 10 2 0 0 0 0 0 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 1 1 0 2 1 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 28 6 7 5 9 5 4 4 6 2 2 1 1 4 6 3 0 0 0 0 0 0
Cytoplasm 241 483 607 474 486 571 610 666 503 478 415 478 406 470 457 98 124 63 232 482 630 239 408 331
Endoplasmic Reticulum 0 5 7 4 12 10 2 1 3 1 0 2 2 1 3 4 9 1 0 1 7 9 11 10
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 3 1 2 3
Golgi 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 2 2 0 5 3
Mitochondria 1 0 0 1 8 52 80 37 167 162 183 119 0 4 2 1 1 0 2 1 6 3 7 3
Nucleus 0 1 5 1 3 4 5 17 2 7 9 5 1 1 0 2 0 1 0 2 1 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 3 1 0 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 0 0 0 0 2 2 1 2 2 8 0 0 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 0 2 0 11 13 15 5 40 14 17 11 3 2 7 15 4 1 0 0 3 4 5 8 10
Unique Cell Count 241 489 626 492 514 625 667 735 601 585 549 577 412 480 472 104 128 66 241 497 661 273 457 382
Labelled Cell Count 244 495 654 497 530 660 719 772 701 682 640 632 416 485 480 113 142 69 241 497 661 273 457 382


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 9.9 8.9 8.2 7.2 5.3 4.9 5.3 4.3 4.0 3.8 4.2 8.8 8.4 8.2 10.7 11.3 10.6 8.6 8.6 9.0
Std Deviation (1e-4) 1.3 1.4 1.0 1.2 1.1 0.9 0.9 1.0 0.9 0.9 0.7 0.9 1.3 1.3 1.2 1.8 1.8 1.9 1.7 1.2 1.5
Intensity Change (Log2) – – – -0.12 -0.29 -0.75 -0.85 -0.75 -1.06 -1.17 -1.24 -1.07 -0.02 -0.08 -0.12 0.26 0.35 0.25 -0.04 -0.04 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -3.1 -3.0 -4.1 -3.4 -4.5 0 0 0 0 -3.8 -4.4 -4.4 0 0.1 0
Cytoplasm -0.6 -2.0 -4.2 -4.2 -4.7 -7.9 -8.7 -10.9 -8.2 1.6 1.0 -0.1 -1.4 -0.1 -0.7
Endoplasmic Reticulum 0 1.6 0.7 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.4 8.9 5.7 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 2.2 0 0.7 1.3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.0 3.9 0 5.9 3.8 4.3 3.6 0 0 0 4.5 0 0 0