Standard name | |
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Human Ortholog | |
Description | AAA ATPase; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity during heat stress, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 3 | 4 | 12 | 6 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 1 | 2 | 5 | 9 |
Bud | 4 | 1 | 4 | 1 | 1 | 0 | 2 | 0 | 0 | 4 | 2 | 3 | 3 | 0 | 1 | 0 | 0 | – | 2 | 9 | 1 | 2 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 10 | 11 | 14 | 12 | 5 | 6 | 7 | 8 | 10 | 7 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 275 | 357 | 239 | 145 | 205 | 189 | 211 | 220 | 155 | 156 | 109 | 109 | 201 | 201 | 184 | 12 | 12 | – | 191 | 373 | 285 | 137 | 69 | 91 |
Endoplasmic Reticulum | 58 | 95 | 176 | 89 | 102 | 76 | 145 | 121 | 56 | 61 | 22 | 44 | 105 | 88 | 67 | 0 | 0 | – | 128 | 82 | 171 | 164 | 198 | 214 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 3 | 1 | 8 | 19 |
Golgi | 0 | 0 | 0 | 2 | 3 | 1 | 3 | 9 | 4 | 8 | 5 | 9 | 1 | 2 | 2 | 0 | 2 | – | 1 | 2 | 1 | 3 | 31 | 51 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 2 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 3 | 3 | 2 | 5 | 0 | 0 | 2 | 0 | 0 | – | 6 | 14 | 8 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 10 | 11 | 4 | 3 | 3 |
Nucleolus | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 3 | 2 | 9 | 4 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 6 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 9 |
Vac/Vac Membrane | 2 | 8 | 31 | 21 | 13 | 14 | 14 | 40 | 18 | 30 | 46 | 56 | 9 | 39 | 56 | 4 | 4 | – | 10 | 35 | 27 | 21 | 13 | 19 |
Unique Cell Count | 318 | 442 | 434 | 250 | 312 | 273 | 368 | 383 | 241 | 267 | 205 | 225 | 307 | 320 | 299 | 16 | 16 | 355 | 543 | 526 | 357 | 353 | 446 | |
Labelled Cell Count | 340 | 462 | 460 | 272 | 339 | 294 | 383 | 403 | 249 | 277 | 221 | 244 | 320 | 331 | 314 | 17 | 18 | 355 | 543 | 526 | 357 | 353 | 446 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 51.4 | 51.6 | 40.6 | 38.8 | 47.1 | 49.2 | 52.6 | 51.2 | 47.2 | 42.7 | 39.7 | 43.6 | 55.5 | 58.1 | 56.2 | 46.4 | 80.0 | – | 44.5 | 48.2 | 52.2 |
Std Deviation (1e-4) | 10.0 | 12.2 | 9.8 | 9.0 | 12.6 | 13.2 | 12.1 | 13.4 | 13.2 | 12.3 | 13.0 | 12.7 | 11.7 | 13.0 | 12.6 | 27.8 | 20.6 | – | 8.9 | 12.0 | 12.1 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.22 | 0.28 | 0.37 | 0.34 | 0.22 | 0.07 | -0.03 | 0.1 | 0.45 | 0.52 | 0.47 | 0.19 | 0.98 | – | 0.13 | 0.25 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 1.5 | 1.7 | 1.6 | -1.0 | -0.8 | 0.5 | 0.6 | 1.7 | 0.6 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | 0.7 | 2.9 | 3.8 | 0.6 | 0.7 | 2.3 | 0.9 | -0.4 | -1.6 | 2.8 | 2.1 | 1.7 | 1.6 | 1.6 | – |
Endoplasmic Reticulum | -1.3 | -2.2 | -3.4 | -0.3 | -2.7 | -4.5 | -4.8 | -7.6 | -5.4 | -1.8 | -3.7 | -5.1 | -3.3 | -3.3 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | 0.6 | -1.7 | -1.1 | -2.0 | 1.7 | 0.2 | 1.9 | 5.5 | 6.4 | -2.5 | 2.4 | 4.8 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 63.4684 | 76.7153 | 72.8325 | 62.5115 | 62.4903 | 75.015 | 73.7544 | 91.6667 | 81.0119 | 73.9792 | 78.9001 | 84.9777 | 66.0756 | 85.1031 | 80.4133 | 74.4077 | 76.7496 | 80.7251 |
Actin | 0.0026 | 0.001 | 0.0062 | 0.0121 | 0.0056 | 0.0004 | 0.0068 | 0.0007 | 0.0013 | 0.0071 | 0.0007 | 0.0004 | 0.016 | 0.0001 | 0.0054 | 0.0026 | 0.0002 | 0.0005 |
Bud | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0008 | 0.0043 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0009 |
Bud Periphery | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0012 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0005 | 0 | 0.0002 | 0.0003 | 0.0006 | 0 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0045 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0022 | 0.0009 | 0.0008 | 0.0026 | 0.0002 | 0.0006 |
Cytoplasm | 0.0317 | 0.075 | 0.0386 | 0.0314 | 0.0538 | 0.0424 | 0.028 | 0.0899 | 0.0576 | 0.047 | 0.0678 | 0.0426 | 0.0343 | 0.0828 | 0.0343 | 0.0442 | 0.051 | 0.0351 |
Cytoplasmic Foci | 0.0021 | 0 | 0.0012 | 0.0006 | 0.0101 | 0 | 0.0013 | 0.0001 | 0.0076 | 0.0001 | 0.0002 | 0.0001 | 0.015 | 0.0002 | 0.0016 | 0.0077 | 0.0141 | 0.0032 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0016 | 0.0018 | 0.0005 | 0.0079 | 0.0062 | 0.0017 | 0.0025 | 0.0011 | 0.0016 | 0.0011 | 0.0013 | 0.0007 | 0.0041 | 0.0006 | 0.0025 | 0.0011 | 0.0013 | 0.0016 |
Endosome | 0.004 | 0.0011 | 0.0051 | 0.0186 | 0.0112 | 0.0001 | 0.0022 | 0.0001 | 0.0028 | 0.0011 | 0.0002 | 0.0001 | 0.0103 | 0.0001 | 0.0006 | 0.002 | 0.0003 | 0.0006 |
Golgi | 0.0004 | 0.0002 | 0.0009 | 0.0037 | 0.0013 | 0 | 0.0007 | 0 | 0.0005 | 0.0005 | 0 | 0 | 0.0027 | 0 | 0.0003 | 0.0009 | 0.0001 | 0.0001 |
Lipid Particles | 0.0011 | 0.0001 | 0.0009 | 0.0006 | 0.001 | 0 | 0.0009 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0 | 0.0092 | 0.0001 | 0.0011 | 0.0067 | 0.0056 | 0.0011 |
Mitochondria | 0.0064 | 0.0007 | 0.0028 | 0.0035 | 0.0043 | 0.0002 | 0.0039 | 0.0009 | 0.0012 | 0.0022 | 0.001 | 0.0006 | 0.0065 | 0.0011 | 0.0048 | 0.0027 | 0.003 | 0.0006 |
None | 0.0063 | 0.0001 | 0.0002 | 0.0004 | 0.0031 | 0 | 0.003 | 0.0006 | 0.0034 | 0.0005 | 0.0002 | 0.0002 | 0.0075 | 0.0002 | 0.0011 | 0.0032 | 0.0081 | 0.0019 |
Nuclear Periphery | 0.3487 | 0.2871 | 0.3345 | 0.3545 | 0.3398 | 0.2796 | 0.3109 | 0.2581 | 0.2826 | 0.3193 | 0.2727 | 0.2202 | 0.3163 | 0.2124 | 0.2966 | 0.2871 | 0.2649 | 0.271 |
Nucleolus | 0.0077 | 0.0016 | 0.0042 | 0.0004 | 0.0063 | 0.0007 | 0.013 | 0.005 | 0.0077 | 0.0208 | 0.006 | 0.0116 | 0.015 | 0.0074 | 0.0052 | 0.0131 | 0.017 | 0.0085 |
Nucleus | 0.5692 | 0.6281 | 0.5977 | 0.5606 | 0.544 | 0.6702 | 0.6134 | 0.6384 | 0.6228 | 0.5946 | 0.6442 | 0.7164 | 0.5156 | 0.6846 | 0.6357 | 0.6011 | 0.6255 | 0.6688 |
Peroxisomes | 0.0002 | 0 | 0.0018 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0062 | 0 | 0.0001 | 0.0033 | 0.0004 | 0.0001 |
Punctate Nuclear | 0.0035 | 0.0004 | 0.0004 | 0.0015 | 0.0052 | 0.0004 | 0.0052 | 0.0007 | 0.0059 | 0.0027 | 0.002 | 0.0025 | 0.0063 | 0.0006 | 0.0008 | 0.0076 | 0.0039 | 0.0016 |
Vacuole | 0.0049 | 0.0009 | 0.0017 | 0.0011 | 0.0026 | 0.001 | 0.0026 | 0.0013 | 0.0012 | 0.0005 | 0.0013 | 0.0016 | 0.0129 | 0.0035 | 0.0044 | 0.0077 | 0.0017 | 0.0021 |
Vacuole Periphery | 0.0077 | 0.0014 | 0.002 | 0.0021 | 0.0033 | 0.0015 | 0.0031 | 0.0009 | 0.0012 | 0.0018 | 0.0015 | 0.0018 | 0.0082 | 0.0048 | 0.0042 | 0.0053 | 0.0021 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 440.6139 | 450.7126 | 494.3763 | 533.245 | 484.8566 | 425.1999 | 477.8844 | 494.7801 | 587.4321 | 476.504 |
Translational Efficiency | 1.9505 | 1.8765 | 1.6896 | 1.8665 | 1.5514 | 1.9659 | 1.7996 | 1.6836 | 1.55 | 1.6148 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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