Standard name
Description AAA ATPase; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity during heat stress, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.81 0.55 0.58 0.66 0.69 0.57 0.57 0.64 0.58 0.53 0.48 0.65 0.63 0.62 0.75 0.75 0.54 0.69 0.54 0.39 0.2 0.2
Endoplasmic Reticulum 0.18 0.21 0.41 0.36 0.33 0.28 0.39 0.32 0.23 0.23 0.11 0.2 0.34 0.28 0.22 0 0 0.36 0.15 0.33 0.46 0.56 0.48
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0.09 0.12
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.07 0.08 0 0.05 0 0.1 0.07 0.11 0.22 0.25 0 0.12 0.19 0.25 0.25 0 0.06 0.05 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 2 3 3 4 12 6 0 0 0 0 0 1 3 1 2 5 9
Bud 4 1 4 1 1 0 2 0 0 4 2 3 3 0 1 0 0 2 9 1 2 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 10 11 14 12 5 6 7 8 10 7 1 1 1 0 0 0 0 0 0 0 0
Cytoplasm 275 357 239 145 205 189 211 220 155 156 109 109 201 201 184 12 12 191 373 285 137 69 91
Endoplasmic Reticulum 58 95 176 89 102 76 145 121 56 61 22 44 105 88 67 0 0 128 82 171 164 198 214
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 1 8 19
Golgi 0 0 0 2 3 1 3 9 4 8 5 9 1 2 2 0 2 1 2 1 3 31 51
Mitochondria 0 0 0 0 0 0 0 0 0 1 4 1 0 0 0 0 0 0 0 1 1 0 2
Nucleus 0 0 0 1 0 2 0 2 3 3 2 5 0 0 2 0 0 6 14 8 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 10 11 4 3 3
Nucleolus 0 0 0 2 0 0 1 2 3 2 9 4 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 6
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 9
Vac/Vac Membrane 2 8 31 21 13 14 14 40 18 30 46 56 9 39 56 4 4 10 35 27 21 13 19
Unique Cell Count 318 442 434 250 312 273 368 383 241 267 205 225 307 320 299 16 16 355 543 526 357 353 446
Labelled Cell Count 340 462 460 272 339 294 383 403 249 277 221 244 320 331 314 17 18 355 543 526 357 353 446


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 51.4 51.6 40.6 38.8 47.1 49.2 52.6 51.2 47.2 42.7 39.7 43.6 55.5 58.1 56.2 46.4 80.0 44.5 48.2 52.2
Std Deviation (1e-4) 10.0 12.2 9.8 9.0 12.6 13.2 12.1 13.4 13.2 12.3 13.0 12.7 11.7 13.0 12.6 27.8 20.6 8.9 12.0 12.1
Intensity Change (Log2) -0.06 0.22 0.28 0.37 0.34 0.22 0.07 -0.03 0.1 0.45 0.52 0.47 0.19 0.98 0.13 0.25 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 1.7 1.6 -1.0 -0.8 0.5 0.6 1.7 0.6 0 0 0 0 0
Cytoplasm 0.7 2.9 3.8 0.6 0.7 2.3 0.9 -0.4 -1.6 2.8 2.1 1.7 1.6 1.6
Endoplasmic Reticulum -1.3 -2.2 -3.4 -0.3 -2.7 -4.5 -4.8 -7.6 -5.4 -1.8 -3.7 -5.1 -3.3 -3.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 -1.7 -1.1 -2.0 1.7 0.2 1.9 5.5 6.4 -2.5 2.4 4.8 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc48-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available