Standard name
Description Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog FXN is mutated in Friedrich's ataxia; human FTL gene can complement yeast yfh1 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0.05 0.05 0 0.05 0 0 0 0 0.11 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.05 0 0.09 0.12 0.12 0.05 0.14 0 0 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0 0 0.05
Golgi 0 0 0.06 0.05 0.16 0 0 0 0 0 0 0.11 0.13 0.15 0.27 0.38 0.35 0 0.13 0 0.05 0.06 0.08
Mitochondria 0.92 0.99 0.79 0.84 0.6 0.84 0.69 0.85 0.86 0.89 0.91 0.92 0.87 0.79 0.15 0.08 0.09 0.84 0.73 0.82 0.73 0.69 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0 0.05 0 0.09 0 0.06 0.06 0 0 0.09 0.13 0.1 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.07 0 0 0.06 0.06 0.06 0 0 0 0.09 0 0.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0.09 0.06 0 0.06 0 0 0 0 0 0.07 0.1 0.05 0.05 0 0.07 0 0.05 0.11 0.11 0.09
SpindlePole 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.12 0.1 0.08 0.21 0.08 0.11 0.06 0 0.05 0.06 0.07 0.08 0.07 0.16 0.17 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 1 1 0 1 1 0 1 1 2 6 12 11 2 0 0 5 15 14
Bud 1 1 2 6 2 10 5 8 5 8 6 1 3 3 4 6 8 0 0 0 1 1 4
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0
Bud Site 1 0 0 0 0 0 0 0 3 3 4 0 0 0 0 0 4
Cell Periphery 3 0 4 12 7 5 1 4 4 6 4 10 13 13 10 14 6 0 0 0 0 0 0
Cytoplasm 1 1 3 4 7 20 18 17 13 13 5 4 4 7 13 9 3 0 0 0 0 1 2
Endoplasmic Reticulum 2 4 0 1 0 0 1 1 1 1 4 1 5 2 6 2 3 0 0 0 0 1 0
Endosome 15 0 11 29 26 16 52 11 8 5 5 4 23 34 6 4 2 10 3 6 7 18 20
Golgi 6 0 7 11 34 0 13 2 6 2 3 26 60 66 32 51 38 9 10 2 13 29 36
Mitochondria 277 67 95 199 127 264 253 271 290 253 272 218 396 348 18 11 10 258 62 121 188 352 283
Nucleus 1 0 0 1 1 12 6 4 1 5 2 0 0 0 4 0 0 0 0 0 0 1 0
Nuclear Periphery 21 2 3 5 1 6 20 14 30 10 19 14 12 6 11 17 11 2 0 0 2 4 5
Nucleolus 16 1 2 3 3 23 16 13 21 17 18 4 16 9 10 5 11 0 0 0 2 4 5
Peroxisomes 7 1 1 21 13 2 23 7 3 3 3 8 34 43 6 7 4 21 0 7 27 56 37
SpindlePole 1 0 4 11 9 6 26 8 10 9 5 0 7 10 2 2 6 0 0 1 1 3 3
Vac/Vac Membrane 28 8 12 19 45 25 40 18 7 13 17 16 37 29 19 23 22 0 3 4 2 9 15
Unique Cell Count 300 68 121 238 211 313 367 317 338 284 300 238 455 438 117 134 109 309 85 148 257 509 436
Labelled Cell Count 380 85 144 323 277 390 476 378 403 349 367 307 611 572 147 163 139 309 85 148 257 509 436


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 6.9 7.2 7.4 8.9 5.8 7.5 6.9 7.0 6.8 6.9 8.6 8.7 9.0 11.0 11.2 11.0 7.9 7.9 8.3
Std Deviation (1e-4) 1.7 1.2 1.3 1.6 1.9 1.7 1.6 2.0 1.6 1.8 1.7 1.7 1.6 1.7 2.7 2.5 2.5 1.9 1.5 2.1
Intensity Change (Log2) 0.04 0.3 -0.3 0.05 -0.06 -0.05 -0.09 -0.07 0.26 0.27 0.31 0.61 0.63 0.6 0.14 0.12 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 3.4 3.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.8 0 0 0 0 0 0 0 0 0 0 1.7 2.2 0
Cytoplasm 0 0 1.6 1.1 1.3 0 0 0 0 0 0 2.7 1.6 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.9 0.9 -1.5 1.4 -2.4 -3.2 0 0 -3.3 -1.7 -0.5 -1.2 -2.1 -2.4
Golgi -0.5 2.8 0 0 0 0 0 0 1.6 2.3 2.7 4.5 6.1 5.6
Mitochondria 1.2 -3.4 1.4 -2.0 1.8 1.9 2.8 3.4 3.5 2.3 0.2 -9.7 -11.4 -10.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.3 0 2.3 0 1.6 1.4 0 0 2.3 3.0 2.4
Nucleolus 0 0 2.3 0 0 2.0 1.9 1.9 0 0 0 2.4 0 2.8
Peroxisomes 3.0 2.3 0 2.4 0 0 0 0 0 2.7 3.3 0 0 0
SpindlePole 0.6 0 0 1.5 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 2.7 -0.6 0.3 -1.6 -3.7 -2.0 -1.6 -1.1 -0.6 -1.2 1.4 1.7 2.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yfh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available