Standard name
Description Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role
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Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.08 0.05 0.05 0.05 0.06 0 0 0 0 0.19 0.21 0.19 0.12 0.14 0.15 0 0 0 0 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0 0.06 0.10 0.11 0.18 0.38 0.27 0.44 0.51 0.50 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.89 0.86 0.85 0.84 0.85 0.72 0.80 0.80 0.76 0.68 0.82 0.70 0.59 0.60 0.80 0.72 0.74 0.89 0.88 0.81 0.76 0.63 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0.06 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.10 0 0 0.05 0 0.06 0 0 0 0 0 0.19 0.20 0.25 0.09 0.15 0.14 0.05 0.06 0.08 0.17 0.23 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1
Bud 0 1 1 0 0 0 1 5 1 3 4 4 0 0 0 0 1 0 4 3 2 2 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 7
Bud Site 0 0 0 0 0 3 6 18 6 4 4 22 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 10 13 11 10 12 13 24 7 13 4 12 36 62 53 42 27 24 1 3 4 2 10 16
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 0 0 0 1 1 1 6 5 1 0 0 0 0 0 1
Endosome 0 1 0 0 4 0 1 0 0 0 0 0 0 6 5 4 2 0 2 3 1 2 3 5
Golgi 0 0 0 1 1 0 1 0 0 0 0 0 1 0 1 4 1 2 1 1 1 0 0 0
Mitochondria 63 10 10 14 24 44 91 110 108 159 125 130 0 2 2 7 2 1 3 2 1 0 6 1
Nucleus 243 325 133 115 175 202 172 325 197 238 171 252 132 173 172 282 142 117 238 327 135 107 193 101
Nuclear Periphery 0 0 1 1 2 6 4 13 2 4 5 3 0 0 0 1 0 0 0 1 0 0 0 0
Nucleolus 0 0 1 1 0 8 10 23 14 18 18 12 0 1 1 1 0 0 1 0 0 0 6 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 1 2 0 0 0 1 0 0 0 0 0 1 0 0 2 0 0 7 5
Vac/Vac Membrane 8 36 6 5 11 10 15 6 9 8 10 9 35 58 71 33 30 22 14 21 13 23 69 73
Unique Cell Count 266 365 155 135 209 239 239 407 247 314 251 309 188 291 286 353 197 158 270 373 167 142 309 226
Labelled Cell Count 318 383 167 149 227 286 316 524 344 447 342 444 205 304 306 380 214 167 270 373 167 142 309 226

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 6.1 5.9 6.2 5.4 5.2 4.7 4.8 4.6 4.3 4.5 4.9 6.5 6.4 6.3 10.8 12.3 12.4 5.1 5.2 6.1
Std Deviation (1e-4) 0.8 1.0 2.6 3.3 1.7 1.5 1.9 1.5 1.2 1.1 1.3 2.9 1.6 1.6 1.6 2.4 3.0 3.0 1.3 1.2 1.4
Intensity Change (Log2) – – – 0.1 -0.1 -0.2 -0.3 -0.3 -0.4 -0.5 -0.4 -0.3 0.1 0.1 0.1 0.9 1.1 1.1 -0.2 -0.2 0.0

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 3.3 3.4 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.2 1.6 3.4 0 0 0 0 0 0 0 0 0 -2.6 -2.8 -2.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 -0.3 0 0 0 0 0 0 3.7 3.8 4.9 1.3 3.0 2.3