Standard name | |
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Description | Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 5 | 1 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 3 | 2 | 2 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 18 | 6 | 4 | 4 | 22 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 10 | 13 | 11 | 10 | 12 | 13 | 24 | 7 | 13 | 4 | 12 | 36 | 62 | 53 | 42 | 27 | 24 | 1 | 3 | 4 | 2 | 10 | 16 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 6 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 1 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 4 | 2 | 0 | 2 | 3 | 1 | 2 | 3 | 5 |
Golgi | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 63 | 10 | 10 | 14 | 24 | 44 | 91 | 110 | 108 | 159 | 125 | 130 | 0 | 2 | 2 | 7 | 2 | 1 | 3 | 2 | 1 | 0 | 6 | 1 |
Nucleus | 243 | 325 | 133 | 115 | 175 | 202 | 172 | 325 | 197 | 238 | 171 | 252 | 132 | 173 | 172 | 282 | 142 | 117 | 238 | 327 | 135 | 107 | 193 | 101 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 2 | 6 | 4 | 13 | 2 | 4 | 5 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 8 | 10 | 23 | 14 | 18 | 18 | 12 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 6 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 7 | 5 |
Vac/Vac Membrane | 8 | 36 | 6 | 5 | 11 | 10 | 15 | 6 | 9 | 8 | 10 | 9 | 35 | 58 | 71 | 33 | 30 | 22 | 14 | 21 | 13 | 23 | 69 | 73 |
Unique Cell Count | 266 | 365 | 155 | 135 | 209 | 239 | 239 | 407 | 247 | 314 | 251 | 309 | 188 | 291 | 286 | 353 | 197 | 158 | 270 | 373 | 167 | 142 | 309 | 226 |
Labelled Cell Count | 318 | 383 | 167 | 149 | 227 | 286 | 316 | 524 | 344 | 447 | 342 | 444 | 205 | 304 | 306 | 380 | 214 | 167 | 270 | 373 | 167 | 142 | 309 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.7 | 6.1 | 5.9 | 6.2 | 5.4 | 5.2 | 4.7 | 4.8 | 4.6 | 4.3 | 4.5 | 4.9 | 6.5 | 6.4 | 6.3 | 10.8 | 12.3 | 12.4 | 5.1 | 5.2 | 6.1 |
Std Deviation (1e-4) | 0.8 | 1.0 | 2.6 | 3.3 | 1.7 | 1.5 | 1.9 | 1.5 | 1.2 | 1.1 | 1.3 | 2.9 | 1.6 | 1.6 | 1.6 | 2.4 | 3.0 | 3.0 | 1.3 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.14 | -0.19 | -0.34 | -0.31 | -0.35 | -0.48 | -0.39 | -0.28 | 0.14 | 0.1 | 0.09 | 0.87 | 1.06 | 1.06 | -0.21 | -0.18 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.1 | -1.4 | -1.3 | -1.2 | -1.1 | -2.5 | -1.9 | -3.3 | -2.0 | 2.8 | 3.5 | 2.9 | 1.2 | 1.6 | 1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | 1.6 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.6 | -2.8 | -2.8 |
Nucleus | -0.1 | -0.5 | -0.3 | -3.2 | -1.6 | -1.5 | -2.5 | -4.0 | -1.1 | -3.4 | -5.7 | -5.6 | -1.6 | -3.1 | -2.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.1 | 0.6 | 0.2 | 1.0 | 0 | -0.1 | 0 | 0.1 | -0.6 | 4.2 | 4.6 | 5.5 | 2.1 | 3.5 | 3.1 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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