Standard name
Description Subunit of chromosomal passenger complex (CPC); CPC is comprised of Ipl1p-Sli15p-Bir1p-Nbl1p and regulates chromosome segregation; required for chromosome bi-orientation and for spindle assembly checkpoint activation upon reduced sister kinetochore tension; relative distribution to shortened microtubules increases upon DNA replication stress; sumoylated in an Mms21p-dependent manner; human survivin homolog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.07 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.08 0.05 0.05 – 0.07 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.09 0.18 0.11 0.11 0.12 0.13 0.14 0.09 – 0.1 0.1 0.2 0.24 0.27 0.26 0.14 0.14 0 0 0.05 0 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.39 0.14 0 0 0.16 0 0 0 0 – 0 0 0.33 0.29 0.25 0.26 0.4 0.42 0.14 0.17 0.12 0.12 0.12 0.11
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.53 0.54 0.67 0.2 0.51 0.54 0.52 0.79 – 0.73 0.73 0.15 0.11 0.17 0.11 0.07 0.1 0 0.05 0.09 0 0 0.07
Nucleus 0.09 0.08 0 0.07 0 0.05 0.06 0.09 0.05 – 0.07 0.07 0.06 0.08 0.09 0.09 0.09 0.06 0 0.06 0.06 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.07 0 0.05 0.09 0.13 0.08 0.11 0.09 – 0.09 0.1 0.18 0.1 0.1 0.06 0.06 0.07 0 0.09 0 0 0.05 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.62 0.31 0.46 0.36 0.64 0.44 0.4 0.42 0.26 – 0.37 0.35 0.42 0.39 0.28 0.38 0.46 0.45 0.56 0.42 0.47 0.54 0.57 0.6
Vac/Vac Membrane 0.07 0.11 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0.11 0.12 0.06 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 1 0 1 0 1
Bud 1 1 1 5 8 5 28 13 11 – 11 21 0 0 5 0 0 1 2 2 0 1 3 1
Bud Neck 2 1 4 4 9 1 9 8 5 – 3 7 0 0 1 2 0 1 1 3 0 1 4 5
Bud Site 0 0 1 3 8 12 31 14 15 – 19 29 1 1 3 3 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 9 15 20 19 25 33 51 41 28 – 28 33 30 56 77 91 30 34 3 9 7 8 12 10
Endoplasmic Reticulum 1 1 1 0 0 0 0 0 0 – 0 2 0 2 1 19 9 10 0 0 0 1 0 2
Endosome 77 24 0 3 35 11 10 6 4 – 3 3 51 67 71 89 85 100 37 39 18 23 30 36
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 0 1 0 0 0 0
Mitochondria 9 90 61 120 44 136 216 153 248 – 207 250 23 25 48 37 15 23 7 12 13 7 8 21
Nucleus 17 14 4 13 6 13 23 25 17 – 20 23 9 18 25 32 19 15 5 14 8 5 1 0
Nuclear Periphery 1 0 0 0 0 0 0 0 3 – 1 1 0 1 2 2 2 3 0 0 0 0 0 0
Nucleolus 25 11 1 9 20 34 30 33 29 – 25 34 27 22 29 22 13 17 11 19 6 6 13 9
Peroxisomes 0 1 0 1 14 6 13 5 2 – 2 3 6 6 7 8 7 3 0 1 0 0 3 3
SpindlePole 120 53 51 64 143 118 159 124 82 – 103 118 65 90 78 129 99 107 146 97 68 105 147 194
Vac/Vac Membrane 14 18 0 0 0 1 2 3 3 – 3 4 2 9 18 39 25 13 5 5 6 11 3 5
Unique Cell Count 195 169 112 179 222 267 398 292 315 282 342 153 231 283 344 213 236 262 230 148 196 259 321
Labelled Cell Count 276 229 145 242 312 370 572 425 447 425 528 214 298 365 474 305 329 262 230 148 196 259 321


Microtubule, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 3.4 3.0 3.3 3.7 3.2 3.0 3.2 2.7 – 2.8 2.5 4.2 4.4 4.0 5.1 5.8 5.9 4.6 4.4 4.7
Std Deviation (1e-4) 0.6 0.7 0.5 1.3 1.4 1.2 1.3 1.1 0.9 – 1.0 1.0 1.0 1.4 1.2 1.2 1.6 1.7 1.8 1.9 1.6
Intensity Change (Log2) – – – 0.13 0.29 0.08 0.01 0.1 -0.15 – -0.13 -0.28 0.47 0.55 0.39 0.75 0.95 0.98 0.62 0.56 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.7 3.8 –