Standard name
Description Cytosolic seryl-tRNA synthetase; class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.12 0.26 0.16 0.25 0.19 0.25 0.42 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 1.0 0.98 0.97 0.95 0.84 0.92 0.88 0.9 0.88 0.95 – 0.94 0.98 0.97 0.97 0.98 0.97 0.92 0.91 0.88 0.94 0.92 0.79
Endoplasmic Reticulum 0 0 0 0 0.05 0 0 0 0 0.06 0 – 0.06 0 0 0.05 0 0 0 0 0 0 0 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 2 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 0 0 0 0 – 0 0 1 0 0 1 2 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 5 5 16 19 13 4 8 – 0 2 2 13 7 5 0 0 2 0 0 0
Cytoplasm 91 16 42 63 41 16 92 68 63 14 18 – 73 160 162 397 257 191 97 37 58 70 88 105
Endoplasmic Reticulum 1 0 1 1 2 0 3 3 0 1 0 – 5 4 3 21 7 5 0 0 0 1 1 13
Endosome 1 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 3 0 1 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 1 0 1 1 0 0 – 0 0 1 0 1 1 0 0 0 0 0 3
Nucleus 3 0 0 0 0 0 0 0 2 0 0 – 0 1 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 1 0 0 1 0 0 0 0 – 0 0 0 3 2 4 1 0 0 1 0 1
Unique Cell Count 97 16 43 65 43 19 100 77 70 16 19 78 164 167 410 262 196 106 41 66 75 96 134
Labelled Cell Count 99 16 44 66 48 22 114 91 80 19 26 78 167 169 439 274 210 106 41 66 75 96 134


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 54.6 56.5 47.8 40.6 42.3 33.9 36.7 31.1 33.7 31.1 30.6 – 59.7 59.3 54.7 51.8 61.4 64.6 58.0 53.5 57.7
Std Deviation (1e-4) 17.9 6.3 16.0 15.4 11.0 12.7 9.3 12.3 9.2 4.1 11.6 – 13.4 12.7 12.8 13.7 16.4 20.2 18.5 20.6 22.7
Intensity Change (Log2) – – – -0.24 -0.18 -0.49 -0.38 -0.62 -0.51 -0.62 -0.65 – 0.32 0.31 0.19 0.12 0.36 0.43 0.28 0.16 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 0 0 2.3 3.2 2.5 0 0 – 0 0 0 0 0 0
Cytoplasm -0.2 -0.6 -2.0 -1.3 -1.8 -1.5 -1.6 -0.6 – -1.0 0 -0.2 -0.3 0.2 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0191 0.0157 0.0202 0.0006 0.0012 0.0216 0.0005 0.0002 0.0266 0.0516 0 0.0061 0.0617 0.0009 0.0128 0.0397 0.0009 0.0027
Bud 0.0033 0.0007 0.0012 0.0009 0.0011 0.0011 0.0003 0.0005 0.0033 0.0052 0.0005 0.0017 0.0032 0.0003 0.0009 0.0019 0.0004 0.0004
Bud Neck 0.0016 0.0006 0.0013 0.0004 0.0012 0.0029 0.0013 0.0002 0.0017 0.0005 0.0002 0.002 0.0023 0.0006 0.0007 0.0043 0.0022 0.0018
Bud Periphery 0.0053 0.0007 0.0012 0.0002 0.0006 0.0009 0.0006 0.0001 0.0035 0.0058 0.0001 0.001 0.0034 0.0003 0.0004 0.0012 0.0002 0.0003
Bud Site 0.0038 0.0038 0.0017 0.0001 0.0014 0.0004 0 0.0003 0.0393 0.0059 0.0001 0.0004 0.0058 0.0006 0.0014 0.0202 0.0001 0.0005
Cell Periphery 0.0269 0.0035 0.0091 0.0003 0.0023 0.0003 0.0011 0.0007 0.0017 0.0006 0.0002 0.0002 0.0046 0.007 0.0008 0.0007 0.002 0.001
Cytoplasm 0.5022 0.916 0.8409 0.8276 0.7232 0.8625 0.9536 0.9924 0.8277 0.8211 0.813 0.8145 0.6919 0.9684 0.9165 0.6717 0.8833 0.8253
Cytoplasmic Foci 0.0331 0.0026 0.0016 0.0023 0.0313 0.0069 0.0017 0.0007 0.0044 0.0031 0.0027 0.0116 0.0168 0.0006 0.0076 0.0527 0.0023 0.0223
Eisosomes 0.0004 0.0001 0.0008 0 0.0008 0 0 0 0.0005 0.0003 0 0 0.0016 0 0.0001 0.0005 0 0.0003
Endoplasmic Reticulum 0.0985 0.0164 0.0458 0.0357 0.0042 0.0288 0.0324 0.0007 0.0033 0.0137 0.0012 0.0098 0.0397 0.0066 0.0062 0.0022 0.0328 0.0529
Endosome 0.0311 0.0021 0.0034 0.016 0.0056 0.0228 0.0025 0.0006 0.0157 0.0251 0.0185 0.0808 0.0178 0.0004 0.0068 0.0189 0.0106 0.0107
Golgi 0.0085 0.0037 0.0024 0.0004 0.001 0.0096 0.0008 0.0001 0.0173 0.0085 0.0002 0.0214 0.0049 0.0001 0.0028 0.0288 0.0005 0.0057
Lipid Particles 0.0262 0.0018 0.001 0.0001 0.012 0.0023 0 0 0.0014 0.0008 0.0001 0.0002 0.0052 0.0003 0.0018 0.0205 0.0009 0.0221
Mitochondria 0.0311 0.0034 0.0089 0.0006 0.0118 0.0033 0.0011 0 0.044 0.0241 0.0001 0.0309 0.0403 0.0001 0.009 0.0178 0.0002 0.0231
None 0.0099 0.0004 0.0005 0.0003 0.0321 0.0007 0.0005 0.0001 0.0003 0.0011 0.0001 0.0008 0.0076 0.0001 0.0018 0.0006 0.0008 0.002
Nuclear Periphery 0.0448 0.003 0.0084 0.0076 0.0062 0.0172 0.0005 0.0003 0.0009 0.0116 0.0054 0.0036 0.0173 0.0018 0.0017 0.0028 0.0084 0.0023
Nucleolus 0.0037 0.0003 0.0001 0.0001 0.0331 0.0002 0 0 0.0002 0.0002 0 0.0001 0.0038 0 0.0002 0.0025 0 0.0066
Nucleus 0.021 0.0025 0.0027 0.003 0.0156 0.0028 0.0005 0.0005 0.001 0.0034 0.0023 0.0029 0.0122 0.0011 0.0017 0.002 0.0018 0.0021
Peroxisomes 0.0098 0.0006 0.0004 0 0.0011 0.0012 0 0 0.0006 0.0003 0 0.0005 0.0014 0 0.0014 0.0211 0 0.0029
Punctate Nuclear 0.0058 0.0008 0.0001 0.0001 0.0571 0.0029 0 0 0.0001 0.0004 0 0.0003 0.003 0 0.0003 0.0087 0.0002 0.0017
Vacuole 0.0935 0.0184 0.0424 0.0738 0.0456 0.0082 0.002 0.0023 0.0048 0.0115 0.1277 0.0051 0.0454 0.0102 0.022 0.0459 0.0436 0.0088
Vacuole Periphery 0.0204 0.0026 0.0059 0.0301 0.0114 0.0034 0.0003 0.0003 0.0016 0.0052 0.0275 0.006 0.0102 0.0006 0.0033 0.0353 0.0088 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 384.8808 354.3569 381.5119 409.2996 375.9348 331.8702 354.3408 346.4978 350.1017 354.2133
Translational Efficiency 1.8649 2.1444 1.9735 1.9726 1.8528 2.045 1.889 1.822 1.7832 1.8101

Hit Data

Dataset Hit
Protein Abundance –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘