Standard name | |
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Description | P-type ATPase sodium pump; involved in Na+ and Li+ efflux to allow salt tolerance |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 37 | 67 | 81 | 73 | 58 | 101 | 101 | 53 | – | 36 | 33 | 50 | 12 | 7 | 7 | 6 | 3 | 7 | 16 | 29 | 28 | 10 | 12 | 15 |
Bud | 8 | 24 | 16 | 31 | 13 | 37 | 47 | 46 | – | 52 | 44 | 46 | 4 | 0 | 1 | 3 | 6 | 5 | 4 | 5 | 4 | 1 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 13 | 10 | 8 | 2 | 2 | 7 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | – | 7 | 3 | 0 | 1 | 0 | 0 | 2 | 4 | 0 | – | – | – | – | – | – |
Cell Periphery | 106 | 103 | 148 | 101 | 59 | 153 | 144 | 62 | – | 49 | 39 | 99 | 34 | 39 | 25 | 4 | 4 | 11 | 163 | 143 | 196 | 71 | 100 | 77 |
Cytoplasm | 88 | 73 | 62 | 59 | 87 | 159 | 192 | 252 | – | 223 | 183 | 235 | 41 | 42 | 43 | 58 | 73 | 38 | 11 | 10 | 1 | 1 | 2 | 0 |
Endoplasmic Reticulum | 3 | 3 | 5 | 1 | 4 | 8 | 15 | 8 | – | 6 | 8 | 10 | 2 | 1 | 0 | 6 | 12 | 9 | 8 | 8 | 7 | 4 | 5 | 4 |
Endosome | 15 | 19 | 54 | 27 | 85 | 24 | 23 | 16 | – | 3 | 0 | 6 | 20 | 24 | 37 | 114 | 109 | 104 | 24 | 57 | 60 | 33 | 47 | 48 |
Golgi | 5 | 6 | 2 | 1 | 9 | 0 | 0 | 1 | – | 0 | 0 | 1 | 2 | 8 | 15 | 4 | 3 | 6 | 20 | 40 | 19 | 15 | 30 | 28 |
Mitochondria | 68 | 163 | 163 | 132 | 113 | 269 | 361 | 465 | – | 439 | 340 | 368 | 55 | 31 | 21 | 22 | 30 | 19 | 8 | 14 | 6 | 10 | 14 | 13 |
Nucleus | 0 | 1 | 0 | 2 | 2 | 1 | 3 | 9 | – | 5 | 7 | 5 | 0 | 1 | 2 | 5 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 10 | 19 | 27 | 3 | 4 | 21 | 46 | 35 | – | 58 | 30 | 54 | 14 | 6 | 13 | 11 | 17 | 18 | 1 | 2 | 3 | 1 | 2 | 1 |
Nucleolus | 16 | 25 | 26 | 17 | 15 | 48 | 75 | 91 | – | 102 | 71 | 93 | 32 | 26 | 43 | 30 | 58 | 45 | 2 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 11 | 29 | 125 | 92 | 27 | 28 | 47 | 19 | – | 9 | 3 | 11 | 44 | 11 | 21 | 29 | 16 | 24 | 30 | 66 | 122 | 73 | 117 | 113 |
SpindlePole | 9 | 12 | 30 | 20 | 12 | 14 | 17 | 24 | – | 19 | 10 | 5 | 35 | 26 | 33 | 68 | 107 | 46 | 3 | 7 | 7 | 9 | 6 | 3 |
Vac/Vac Membrane | 5 | 1 | 1 | 2 | 11 | 7 | 22 | 11 | – | 9 | 14 | 23 | 3 | 8 | 6 | 20 | 14 | 9 | 7 | 5 | 3 | 2 | 2 | 4 |
Unique Cell Count | 262 | 360 | 444 | 336 | 367 | 595 | 747 | 840 | 748 | 577 | 711 | 217 | 159 | 183 | 276 | 314 | 240 | 317 | 404 | 474 | 241 | 349 | 325 | |
Labelled Cell Count | 382 | 545 | 740 | 561 | 499 | 871 | 1097 | 1095 | 1018 | 785 | 1006 | 299 | 230 | 267 | 383 | 466 | 345 | 317 | 404 | 474 | 241 | 349 | 325 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 8.5 | 7.7 | 7.3 | 7.5 | 6.6 | 6.9 | 6.7 | – | 5.7 | 5.7 | 6.4 | 9.3 | 9.7 | 9.6 | 6.0 | 6.1 | 8.0 | 9.6 | 9.8 | 10.5 |
Std Deviation (1e-4) | 1.6 | 1.9 | 1.8 | 1.5 | 1.7 | 1.5 | 2.0 | 1.8 | – | 1.9 | 1.8 | 1.8 | 1.8 | 2.2 | 1.9 | 1.3 | 1.3 | 1.7 | 1.8 | 1.9 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.04 | -0.22 | -0.14 | -0.2 | – | -0.42 | -0.44 | -0.25 | 0.28 | 0.33 | 0.33 | -0.35 | -0.33 | 0.06 | 0.32 | 0.35 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.2 | -0.9 | -0.5 | -2.2 | -6.7 | – | -7.5 | -6.3 | -5.8 | -4.4 | -4.2 | -4.7 | -6.4 | -7.5 | -5.7 |
Bud | 3.3 | 0 | 1.9 | 2.0 | 1.5 | – | 2.4 | 2.7 | 2.1 | -1.2 | 0 | 0 | -2.0 | -1.4 | -1.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -5.6 | -2.7 | -5.5 | -12.0 | – | -12.0 | -10.9 | -7.8 | -4.8 | -2.1 | -5.0 | -10.2 | -10.9 | -8.5 |
Cytoplasm | 1.4 | 3.6 | 5.0 | 4.8 | 6.4 | – | 6.2 | 6.6 | 7.2 | 1.6 | 3.6 | 2.9 | 2.5 | 3.3 | 0.7 |
Endoplasmic Reticulum | 0 | 0 | 0.3 | 1.1 | 0 | – | 0 | 0.4 | 0.4 | 0 | 0 | 0 | 0 | 2.5 | 0 |
Endosome | -1.9 | 4.1 | -4.9 | -6.2 | -7.7 | – | -9.2 | -8.6 | -8.4 | -1.1 | 0.9 | 2.6 | 9.0 | 7.4 | 9.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | -1.8 | 2.7 | 3.9 | 6.4 | – | 7.3 | 7.0 | 5.0 | -2.9 | -4.0 | -6.3 | -8.6 | -8.5 | -8.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -3.7 | -3.7 | -1.9 | 0.1 | -1.5 | – | 1.1 | -0.6 | 1.0 | 0.2 | -1.1 | 0.5 | -1.2 | -0.4 | 0.7 |
Nucleolus | -0.5 | -1.1 | 1.4 | 2.5 | 2.9 | – | 4.2 | 3.5 | 3.9 | 3.8 | 4.0 | 6.4 | 2.4 | 5.5 | 5.3 |
Peroxisomes | -0.2 | -7.6 | -10.6 | -10.4 | -14.0 | – | -14.2 | -13.2 | -13.6 | -2.2 | -5.5 | -4.5 | -5.6 | -8.0 | -5.5 |
SpindlePole | -0.5 | -2.2 | -3.5 | -3.8 | -3.3 | – | -3.5 | -4.1 | -5.8 | 3.8 | 3.6 | 4.3 | 6.8 | 9.6 | 4.9 |
Vacuole | 0 | 3.3 | 0 | 3.3 | 0 | – | 0 | 2.9 | 3.5 | 0 | 0 | 0 | 5.4 | 4.1 | 0 |