Standard name
Description P-type ATPase sodium pump; involved in Na+ and Li+ efflux to allow salt tolerance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.19 0.18 0.22 0.16 0.17 0.14 0.06 – 0.05 0.06 0.07 0.06 0 0 0 0 0 0.05 0.07 0.06 0.05 0 0.05
Bud 0 0.07 0 0.09 0 0.06 0.06 0.05 – 0.07 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.4 0.29 0.33 0.3 0.16 0.26 0.19 0.07 – 0.07 0.07 0.14 0.16 0.25 0.14 0 0 0.05 0.52 0.36 0.41 0.3 0.29 0.24
Cytoplasm 0.34 0.2 0.14 0.18 0.24 0.27 0.26 0.3 – 0.3 0.32 0.33 0.19 0.26 0.23 0.21 0.23 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0.05 0.12 0.08 0.23 0 0 0 – 0 0 0 0.09 0.15 0.2 0.41 0.35 0.43 0.08 0.14 0.13 0.14 0.14 0.15
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.08 0 0 0 0.07 0.1 0 0.07 0.09 0.09
Mitochondria 0.26 0.45 0.37 0.39 0.31 0.45 0.48 0.55 – 0.59 0.59 0.52 0.25 0.19 0.11 0.08 0.1 0.08 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0.06 0 0 0 0.06 0 – 0.08 0.05 0.08 0.06 0 0.07 0 0.05 0.07 0 0 0 0 0 0
Nucleolus 0.06 0.07 0.06 0.05 0 0.08 0.1 0.11 – 0.14 0.12 0.13 0.15 0.16 0.23 0.11 0.18 0.19 0 0 0 0 0 0
Peroxisomes 0 0.08 0.28 0.27 0.07 0.05 0.06 0 – 0 0 0 0.2 0.07 0.11 0.11 0.05 0.1 0.1 0.16 0.26 0.31 0.34 0.35
SpindlePole 0 0 0.07 0.06 0 0 0 0 – 0 0 0 0.16 0.16 0.18 0.25 0.34 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 37 67 81 73 58 101 101 53 – 36 33 50 12 7 7 6 3 7 16 29 28 10 12 15
Bud 8 24 16 31 13 37 47 46 – 52 44 46 4 0 1 3 6 5 4 5 4 1 0 3
Bud Neck 0 0 0 0 0 0 1 1 – 1 0 0 0 0 0 1 2 0 13 10 8 2 2 7
Bud Site 1 0 0 0 0 1 3 2 – 7 3 0 1 0 0 2 4 0 – – – – – –
Cell Periphery 106 103 148 101 59 153 144 62 – 49 39 99 34 39 25 4 4 11 163 143 196 71 100 77
Cytoplasm 88 73 62 59 87 159 192 252 – 223 183 235 41 42 43 58 73 38 11 10 1 1 2 0
Endoplasmic Reticulum 3 3 5 1 4 8 15 8 – 6 8 10 2 1 0 6 12 9 8 8 7 4 5 4
Endosome 15 19 54 27 85 24 23 16 – 3 0 6 20 24 37 114 109 104 24 57 60 33 47 48
Golgi 5 6 2 1 9 0 0 1 – 0 0 1 2 8 15 4 3 6 20 40 19 15 30 28
Mitochondria 68 163 163 132 113 269 361 465 – 439 340 368 55 31 21 22 30 19 8 14 6 10 14 13
Nucleus 0 1 0 2 2 1 3 9 – 5 7 5 0 1 2 5 8 4 0 0 0 0 0 0
Nuclear Periphery 10 19 27 3 4 21 46 35 – 58 30 54 14 6 13 11 17 18 1 2 3 1 2 1
Nucleolus 16 25 26 17 15 48 75 91 – 102 71 93 32 26 43 30 58 45 2 1 0 0 0 0
Peroxisomes 11 29 125 92 27 28 47 19 – 9 3 11 44 11 21 29 16 24 30 66 122 73 117 113
SpindlePole 9 12 30 20 12 14 17 24 – 19 10 5 35 26 33 68 107 46 3 7 7 9 6 3
Vac/Vac Membrane 5 1 1 2 11 7 22 11 – 9 14 23 3 8 6 20 14 9 7 5 3 2 2 4
Unique Cell Count 262 360 444 336 367 595 747 840 748 577 711 217 159 183 276 314 240 317 404 474 241 349 325
Labelled Cell Count 382 545 740 561 499 871 1097 1095 1018 785 1006 299 230 267 383 466 345 317 404 474 241 349 325


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 8.5 7.7 7.3 7.5 6.6 6.9 6.7 – 5.7 5.7 6.4 9.3 9.7 9.6 6.0 6.1 8.0 9.6 9.8 10.5
Std Deviation (1e-4) 1.6 1.9 1.8 1.5 1.7 1.5 2.0 1.8 – 1.9 1.8 1.8 1.8 2.2 1.9 1.3 1.3 1.7 1.8 1.9 2.3
Intensity Change (Log2) – – – -0.07 -0.04 -0.22 -0.14 -0.2 – -0.42 -0.44 -0.25 0.28 0.33 0.33 -0.35 -0.33 0.06 0.32 0.35 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 -0.9 -0.5 -2.2 -6.7 – -7.5 -6.3 -5.8 -4.4 -4.2 -4.7 -6.4 -7.5 -5.7
Bud 3.3 0 1.9 2.0 1.5 – 2.4 2.7 2.1 -1.2 0 0 -2.0 -1.4 -1.1
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -5.6 -2.7 -5.5 -12.0 – -12.0 -10.9 -7.8 -4.8 -2.1 -5.0 -10.2 -10.9 -8.5
Cytoplasm 1.4 3.6 5.0 4.8 6.4 – 6.2 6.6 7.2 1.6 3.6 2.9 2.5 3.3 0.7
Endoplasmic Reticulum 0 0 0.3 1.1 0 – 0 0.4 0.4 0 0 0 0 2.5 0
Endosome -1.9 4.1 -4.9 -6.2 -7.7 – -9.2 -8.6 -8.4 -1.1 0.9 2.6 9.0 7.4 9.2
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 -1.8 2.7 3.9 6.4 – 7.3 7.0 5.0 -2.9 -4.0 -6.3 -8.6 -8.5 -8.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -3.7 -3.7 -1.9 0.1 -1.5 – 1.1 -0.6 1.0 0.2 -1.1 0.5 -1.2 -0.4 0.7
Nucleolus -0.5 -1.1 1.4 2.5 2.9 – 4.2 3.5 3.9 3.8 4.0 6.4 2.4 5.5 5.3
Peroxisomes -0.2 -7.6 -10.6 -10.4 -14.0 – -14.2 -13.2 -13.6 -2.2 -5.5 -4.5 -5.6 -8.0 -5.5
SpindlePole -0.5 -2.2 -3.5 -3.8 -3.3 – -3.5 -4.1 -5.8 3.8 3.6 4.3 6.8 9.6 4.9
Vacuole 0 3.3 0 3.3 0 – 0 2.9 3.5 0 0 0 5.4 4.1 0