Standard name | |
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Description | Component of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 22 | 28 | 47 | 42 | 50 | 32 | 36 | 67 | – | 55 | 60 | 67 | 87 | 64 | 33 | 24 | 3 | 9 | 6 | 10 | 17 | 22 | 34 | 16 |
Bud | 1 | 4 | 1 | 1 | 4 | 2 | 3 | 2 | – | 9 | 18 | 12 | 5 | 5 | 6 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 16 | 35 | 48 | 45 | 46 | 43 | 33 | 33 | – | 54 | 46 | 60 | 195 | 232 | 219 | 34 | 4 | 22 | 5 | 10 | 22 | 36 | 88 | 71 |
Cytoplasm | 22 | 27 | 16 | 18 | 21 | 31 | 36 | 45 | – | 36 | 40 | 41 | 65 | 82 | 103 | 24 | 9 | 27 | 1 | 6 | 1 | 2 | 7 | 8 |
Endoplasmic Reticulum | 155 | 135 | 200 | 137 | 241 | 233 | 244 | 224 | – | 258 | 161 | 233 | 742 | 805 | 851 | 1 | 0 | 2 | 188 | 165 | 195 | 283 | 465 | 415 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 |
Golgi | 6 | 0 | 12 | 24 | 38 | 9 | 12 | 3 | – | 0 | 3 | 5 | 12 | 3 | 6 | 3 | 2 | 9 | 0 | 0 | 1 | 3 | 5 | 6 |
Mitochondria | 11 | 5 | 22 | 51 | 45 | 57 | 65 | 80 | – | 140 | 158 | 132 | 0 | 0 | 0 | 14 | 1 | 10 | 2 | 4 | 1 | 8 | 7 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 2 | 2 | 3 | 6 | 10 | – | 23 | 7 | 13 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 0 | 0 | 0 | 3 | 11 | 5 | 22 | – | 8 | 10 | 25 | 10 | 7 | 6 | 7 | 1 | 3 | 3 | 6 | 0 | 1 | 2 | 5 |
Unique Cell Count | 189 | 183 | 241 | 191 | 311 | 298 | 331 | 343 | 411 | 355 | 414 | 911 | 1000 | 1040 | 96 | 17 | 72 | 213 | 211 | 246 | 367 | 620 | 541 | |
Labelled Cell Count | 240 | 236 | 347 | 320 | 452 | 422 | 440 | 489 | 583 | 505 | 589 | 1116 | 1199 | 1227 | 110 | 21 | 82 | 213 | 211 | 246 | 367 | 620 | 541 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.4 | 14.1 | 14.0 | 14.4 | 14.0 | 12.6 | 12.3 | 11.9 | – | 11.2 | 11.2 | 12.1 | 14.8 | 15.4 | 16.1 | 9.8 | 12.3 | 13.9 | 15.3 | 15.5 | 15.7 |
Std Deviation (1e-4) | 1.9 | 2.3 | 2.2 | 2.1 | 2.0 | 2.1 | 2.2 | 2.5 | – | 2.1 | 2.3 | 2.4 | 2.0 | 1.9 | 2.0 | 1.7 | 3.2 | 3.1 | 2.0 | 2.4 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.0 | -0.14 | -0.19 | -0.24 | – | -0.32 | -0.31 | -0.21 | 0.08 | 0.14 | 0.21 | -0.51 | -0.18 | -0.01 | 0.14 | 0.15 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.6 | -1.0 | -2.9 | -2.9 | 0 | – | -2.1 | -0.8 | -1.1 | -4.3 | -6.4 | -9.4 | 1.1 | 0 | -1.4 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | -1.6 | -1.7 | -3.4 | -3.5 | – | -2.3 | -2.3 | -1.8 | 0.5 | 1.1 | 0.4 | 3.0 | 0 | 1.9 |
Cytoplasm | 1.1 | 0.1 | 1.5 | 1.7 | 2.5 | – | 1.0 | 1.9 | 1.4 | 0.3 | 0.8 | 1.6 | 4.7 | 0 | 6.7 |
Endoplasmic Reticulum | -2.8 | -1.6 | -1.4 | -2.6 | -4.7 | – | -5.4 | -9.2 | -7.0 | -0.6 | -0.9 | -0.4 | -13.8 | -7.9 | -12.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.8 | 2.9 | -1.2 | -0.8 | -3.1 | – | 0 | -3.2 | -2.9 | -3.5 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.8 | 1.9 | 3.3 | 3.5 | 4.4 | – | 7.1 | 9.2 | 6.6 | 0 | 0 | 0 | 1.5 | 0 | 1.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 4.0 | – | 0 | 0 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |