Standard name
Description Component of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.15 0.2 0.22 0.16 0.11 0.11 0.2 – 0.13 0.17 0.16 0.1 0.06 0 0.25 0.18 0.12 0 0.05 0.07 0.06 0.06 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.08 0.19 0.2 0.24 0.15 0.14 0.1 0.1 – 0.13 0.13 0.14 0.21 0.23 0.21 0.35 0.24 0.31 0 0.05 0.09 0.1 0.14 0.13
Cytoplasm 0.12 0.15 0.07 0.09 0.07 0.1 0.11 0.13 – 0.09 0.11 0.1 0.07 0.08 0.1 0.25 0.53 0.38 0 0 0 0 0 0
Endoplasmic Reticulum 0.82 0.74 0.83 0.72 0.77 0.78 0.74 0.65 – 0.63 0.45 0.56 0.81 0.81 0.82 0 0 0 0.89 0.79 0.8 0.77 0.75 0.77
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.05 0.13 0.12 0 0 0 – 0 0 0 0 0 0 0 0.12 0.12 0 0 0 0 0 0
Mitochondria 0.06 0 0.09 0.27 0.14 0.19 0.2 0.23 – 0.34 0.45 0.32 0 0 0 0.15 0.06 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 – 0 0 0.06 0 0 0 0.07 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 28 47 42 50 32 36 67 – 55 60 67 87 64 33 24 3 9 6 10 17 22 34 16
Bud 1 4 1 1 4 2 3 2 – 9 18 12 5 5 6 1 1 0 1 0 0 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3 0 1 1 3
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 16 35 48 45 46 43 33 33 – 54 46 60 195 232 219 34 4 22 5 10 22 36 88 71
Cytoplasm 22 27 16 18 21 31 36 45 – 36 40 41 65 82 103 24 9 27 1 6 1 2 7 8
Endoplasmic Reticulum 155 135 200 137 241 233 244 224 – 258 161 233 742 805 851 1 0 2 188 165 195 283 465 415
Endosome 0 0 0 0 0 1 0 2 – 0 2 1 0 1 0 1 0 0 0 0 0 3 2 0
Golgi 6 0 12 24 38 9 12 3 – 0 3 5 12 3 6 3 2 9 0 0 1 3 5 6
Mitochondria 11 5 22 51 45 57 65 80 – 140 158 132 0 0 0 14 1 10 2 4 1 8 7 9
Nucleus 0 0 0 0 1 0 0 1 – 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 2 0 2 2 3 6 10 – 23 7 13 0 0 0 1 0 0 0 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 5 0 0 0 3 11 5 22 – 8 10 25 10 7 6 7 1 3 3 6 0 1 2 5
Unique Cell Count 189 183 241 191 311 298 331 343 411 355 414 911 1000 1040 96 17 72 213 211 246 367 620 541
Labelled Cell Count 240 236 347 320 452 422 440 489 583 505 589 1116 1199 1227 110 21 82 213 211 246 367 620 541


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.4 14.1 14.0 14.4 14.0 12.6 12.3 11.9 – 11.2 11.2 12.1 14.8 15.4 16.1 9.8 12.3 13.9 15.3 15.5 15.7
Std Deviation (1e-4) 1.9 2.3 2.2 2.1 2.0 2.1 2.2 2.5 – 2.1 2.3 2.4 2.0 1.9 2.0 1.7 3.2 3.1 2.0 2.4 2.4
Intensity Change (Log2) – – – 0.04 -0.0 -0.14 -0.19 -0.24 – -0.32 -0.31 -0.21 0.08 0.14 0.21 -0.51 -0.18 -0.01 0.14 0.15 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -1.0 -2.9 -2.9 0 – -2.1 -0.8 -1.1 -4.3 -6.4 -9.4 1.1 0 -1.4
Bud 0 0 0 0 0 – 0 3.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 -1.6 -1.7 -3.4 -3.5 – -2.3 -2.3 -1.8 0.5 1.1 0.4 3.0 0 1.9
Cytoplasm 1.1 0.1 1.5 1.7 2.5 – 1.0 1.9 1.4 0.3 0.8 1.6 4.7 0 6.7
Endoplasmic Reticulum -2.8 -1.6 -1.4 -2.6 -4.7 – -5.4 -9.2 -7.0 -0.6 -0.9 -0.4 -13.8 -7.9 -12.5
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 2.8 2.9 -1.2 -0.8 -3.1 – 0 -3.2 -2.9 -3.5 0 0 0 0 0
Mitochondria 4.8 1.9 3.3 3.5 4.4 – 7.1 9.2 6.6 0 0 0 1.5 0 1.2
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 3.7 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.0 – 0 0 3.9 0 0 0 0 0 0