Standard name
Human Ortholog
Description Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p); this complex forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.13 0.13 0.14 0 0
Bud 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0
Bud Site 0 0 0 0
Cell Periphery 0.15 0.25 0.32 0.29 0.06 0
Cytoplasm 0.27 0.19 0.19 0.18 0.07 0
Endoplasmic Reticulum 0.51 0.05 0.05 0.07 0.68 0
Endosome 0 0 0 0 0 0.33
Golgi 0 0.27 0.19 0.21 0 0
Mitochondria 0.07 0.16 0.17 0.13 0 0.33
Nucleus 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0.09 0.13 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 31 36 31 8 0
Bud 0 1 1 4 2 0
Bud Neck 0 0 0 0 0 0
Bud Site 0 0 0 0
Cell Periphery 24 59 89 62 12 0
Cytoplasm 45 46 53 38 13 0
Endoplasmic Reticulum 83 11 13 16 144 0
Endosome 0 4 6 1 1 1
Golgi 4 64 53 46 3 0
Mitochondria 11 37 48 28 9 1
Nucleus 1 0 1 0 2 0
Nuclear Periphery 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0
SpindlePole 0 2 1 3 0 0
Vac/Vac Membrane 2 17 26 29 9 0
Unique Cell Count 164 236 279 216 212 5
Labelled Cell Count 186 272 327 259 212 5


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 9.8 9.9 10.1
Std Deviation (1e-4) 1.6 1.3 1.4 1.6
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 413.2091 365.3245 262.0503 335.9445 441.5469 363.0596 329.3631 353.1836 317.3866 362.1141
Translational Efficiency 1.8546 1.7435 1.9878 1.3671 1.6778 2.8776 2.2707 1.6891 1.7681 2.4718

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency