Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; deletion confers sensitivity to cationic compounds; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 5 | – | 21 | 5 | 5 | 10 | 3 | 2 | 5 | 5 | 2 | 24 | 72 | 76 | 11 | 13 | 29 | 3 | 1 | 2 | 6 | 9 | 11 |
Bud | 0 | 2 | – | 7 | 0 | 2 | 4 | 0 | 5 | 5 | 3 | 6 | 11 | 2 | 4 | 5 | 3 | 4 | 0 | 2 | 0 | 1 | 3 | 3 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 5 | 6 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 12 | 14 | – | 64 | 30 | 16 | 32 | 18 | 20 | 9 | 18 | 29 | 113 | 300 | 404 | 16 | 13 | 21 | 18 | 6 | 4 | 17 | 84 | 69 |
Cytoplasm | 12 | 32 | – | 57 | 58 | 87 | 185 | 133 | 113 | 134 | 148 | 168 | 97 | 97 | 112 | 56 | 37 | 50 | 4 | 17 | 5 | 5 | 16 | 10 |
Endoplasmic Reticulum | 35 | 21 | – | 76 | 127 | 84 | 102 | 57 | 28 | 29 | 27 | 19 | 138 | 121 | 77 | 6 | 2 | 6 | 28 | 34 | 15 | 87 | 185 | 246 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Golgi | 1 | 0 | – | 0 | 9 | 5 | 3 | 2 | 5 | 4 | 1 | 4 | 8 | 25 | 30 | 53 | 28 | 27 | 0 | 0 | 0 | 0 | 1 | 5 |
Mitochondria | 0 | 5 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 3 | 1 | 0 | 3 | 5 | 7 | 4 | 0 | 1 | 2 | 0 | 2 | 5 |
Nucleus | 0 | 0 | – | 4 | 14 | 24 | 69 | 60 | 28 | 22 | 12 | 15 | 3 | 1 | 1 | 10 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | – | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 11 | 15 | 23 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | – | 9 | 46 | 64 | 154 | 157 | 120 | 139 | 113 | 147 | 20 | 9 | 5 | 65 | 44 | 35 | 27 | 36 | 22 | 164 | 197 | 206 |
Unique Cell Count | 57 | 67 | 183 | 254 | 251 | 480 | 373 | 281 | 314 | 302 | 332 | 330 | 489 | 565 | 197 | 137 | 157 | 85 | 104 | 55 | 292 | 509 | 574 | |
Labelled Cell Count | 63 | 81 | 241 | 289 | 287 | 561 | 430 | 324 | 352 | 331 | 394 | 426 | 642 | 735 | 234 | 158 | 181 | 85 | 104 | 55 | 292 | 509 | 574 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.8 | 13.8 | – | 20.0 | 26.0 | 28.7 | 29.1 | 30.1 | 30.3 | 27.6 | 29.4 | 28.5 | 17.1 | 15.4 | 14.3 | 32.4 | 34.3 | 35.9 | 17.8 | 16.8 | 17.8 |
Std Deviation (1e-4) | 3.4 | 5.0 | – | 4.4 | 6.5 | 7.7 | 8.5 | 8.7 | 8.9 | 8.9 | 8.9 | 8.5 | 3.5 | 2.7 | 2.6 | 10.5 | 11.4 | 10.0 | 4.0 | 4.1 | 3.9 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4705 | 5.6841 | 4.3293 | 4.2054 | 3.6806 | 4.9357 | 4.2166 | 6.361 | 5.2496 | 4.579 | 4.9004 | 6.0899 | 4.7005 | 5.8943 | 5.2148 | 4.5846 | 4.466 | 5.279 |
Actin | 0.0102 | 0.0022 | 0.007 | 0.0046 | 0.0033 | 0.0037 | 0.0054 | 0.0012 | 0.0182 | 0.0147 | 0.0007 | 0.0016 | 0.0288 | 0.0029 | 0.0203 | 0.0242 | 0.022 | 0.0102 |
Bud | 0.0012 | 0.0019 | 0.0049 | 0.0025 | 0.0029 | 0.0016 | 0.0007 | 0.0004 | 0.0019 | 0.0045 | 0.0028 | 0.0005 | 0.0015 | 0.001 | 0.0016 | 0.0015 | 0.0018 | 0.0012 |
Bud Neck | 0.0105 | 0.005 | 0.0144 | 0.0224 | 0.0076 | 0.0144 | 0.0063 | 0.0081 | 0.0187 | 0.0073 | 0.0069 | 0.0059 | 0.0044 | 0.0051 | 0.012 | 0.0294 | 0.0055 | 0.0143 |
Bud Periphery | 0.0067 | 0.0089 | 0.0268 | 0.0191 | 0.0206 | 0.0153 | 0.0048 | 0.0025 | 0.0115 | 0.0086 | 0.0217 | 0.0066 | 0.0096 | 0.0059 | 0.0126 | 0.0115 | 0.0078 | 0.0083 |
Bud Site | 0.0086 | 0.0566 | 0.0346 | 0.0283 | 0.0083 | 0.0032 | 0.0113 | 0.0434 | 0.0166 | 0.0299 | 0.0042 | 0.0013 | 0.0105 | 0.0282 | 0.0137 | 0.0207 | 0.0073 | 0.0024 |
Cell Periphery | 0.3734 | 0.3209 | 0.3527 | 0.329 | 0.3355 | 0.3781 | 0.4469 | 0.4364 | 0.3686 | 0.2659 | 0.3283 | 0.4926 | 0.4102 | 0.3688 | 0.414 | 0.3145 | 0.3029 | 0.3504 |
Cytoplasm | 0.1858 | 0.2473 | 0.168 | 0.1825 | 0.1445 | 0.1878 | 0.1634 | 0.1672 | 0.1265 | 0.14 | 0.2228 | 0.0689 | 0.1474 | 0.1727 | 0.0998 | 0.1053 | 0.1174 | 0.143 |
Cytoplasmic Foci | 0.0101 | 0.0083 | 0.016 | 0.031 | 0.0144 | 0.0045 | 0.0149 | 0.0051 | 0.0096 | 0.0696 | 0.0033 | 0.0006 | 0.006 | 0.0071 | 0.0052 | 0.0015 | 0.0268 | 0.0027 |
Eisosomes | 0.0035 | 0.0005 | 0.0006 | 0.0002 | 0.0015 | 0.0002 | 0.0012 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.0009 | 0.0028 | 0.0005 | 0.0003 | 0.0002 | 0.001 | 0.0004 |
Endoplasmic Reticulum | 0.0388 | 0.0342 | 0.04 | 0.0448 | 0.0223 | 0.0295 | 0.0218 | 0.0135 | 0.0352 | 0.0463 | 0.0075 | 0.0128 | 0.032 | 0.0392 | 0.039 | 0.0105 | 0.0131 | 0.0261 |
Endosome | 0.0312 | 0.0139 | 0.0264 | 0.0437 | 0.0584 | 0.012 | 0.0185 | 0.008 | 0.0175 | 0.0336 | 0.0061 | 0.0009 | 0.012 | 0.014 | 0.0116 | 0.0044 | 0.0349 | 0.007 |
Golgi | 0.009 | 0.0027 | 0.0078 | 0.0133 | 0.0065 | 0.0023 | 0.0078 | 0.0012 | 0.0183 | 0.0215 | 0.0004 | 0.0003 | 0.0047 | 0.0022 | 0.003 | 0.0005 | 0.0078 | 0.0018 |
Lipid Particles | 0.0399 | 0.0594 | 0.0389 | 0.0273 | 0.04 | 0.0395 | 0.0392 | 0.0622 | 0.0544 | 0.028 | 0.0208 | 0.0243 | 0.0556 | 0.077 | 0.0488 | 0.0213 | 0.028 | 0.0323 |
Mitochondria | 0.0074 | 0.0005 | 0.0108 | 0.0048 | 0.002 | 0.0013 | 0.0146 | 0.0001 | 0.0075 | 0.0037 | 0.0001 | 0 | 0.003 | 0.0001 | 0.0008 | 0.0002 | 0.0023 | 0.0009 |
None | 0.0026 | 0.0015 | 0.0092 | 0.0016 | 0.0336 | 0.0054 | 0.0092 | 0.0018 | 0.0027 | 0.0009 | 0.0005 | 0.0003 | 0.0046 | 0.0007 | 0.0016 | 0.0003 | 0.0196 | 0.001 |
Nuclear Periphery | 0.004 | 0.0015 | 0.0021 | 0.002 | 0.0044 | 0.0014 | 0.0023 | 0.001 | 0.002 | 0.002 | 0.0008 | 0.0007 | 0.003 | 0.0017 | 0.0022 | 0.0013 | 0.0037 | 0.0019 |
Nucleolus | 0.0012 | 0.0014 | 0.0014 | 0.0009 | 0.0017 | 0.0006 | 0.0007 | 0.0009 | 0.0023 | 0.0009 | 0.0006 | 0.0004 | 0.0012 | 0.0009 | 0.0009 | 0.0013 | 0.0009 | 0.0006 |
Nucleus | 0.0054 | 0.0022 | 0.0033 | 0.0024 | 0.0029 | 0.002 | 0.0013 | 0.0012 | 0.0021 | 0.0023 | 0.0011 | 0.0007 | 0.0032 | 0.0014 | 0.0015 | 0.0021 | 0.0016 | 0.0013 |
Peroxisomes | 0.0028 | 0.0008 | 0.0081 | 0.0123 | 0.0009 | 0.0003 | 0.0018 | 0.0001 | 0.0052 | 0.0175 | 0 | 0 | 0.0005 | 0.0001 | 0.0038 | 0.0001 | 0.0022 | 0.0004 |
Punctate Nuclear | 0.0004 | 0.0003 | 0.0017 | 0.0008 | 0.0015 | 0.0002 | 0.0014 | 0.0002 | 0.0008 | 0.0014 | 0.0001 | 0 | 0.0013 | 0.0002 | 0.0003 | 0.0001 | 0.0022 | 0.0002 |
Vacuole | 0.2437 | 0.2284 | 0.2228 | 0.2239 | 0.2851 | 0.2945 | 0.2242 | 0.2447 | 0.2783 | 0.2994 | 0.3705 | 0.3797 | 0.2558 | 0.2693 | 0.3049 | 0.4469 | 0.3886 | 0.392 |
Vacuole Periphery | 0.0036 | 0.0014 | 0.0025 | 0.0024 | 0.0024 | 0.0021 | 0.0024 | 0.0005 | 0.0016 | 0.002 | 0.0009 | 0.0008 | 0.0019 | 0.001 | 0.002 | 0.0024 | 0.0026 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.6962 | 19.0298 | 21.9659 | 20.3469 | 13.8001 | 21.2758 | 24.8529 | 23.0869 | 24.6672 | 25.2758 |
Translational Efficiency | 0.9077 | 0.8722 | 0.6737 | 0.835 | 1.1498 | 0.9595 | 0.7232 | 0.6376 | 0.882 | 0.6503 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|