Standard name
Description Protein of unknown function; deletion confers sensitivity to cationic compounds; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.07 0.11 0 0 0 0 0 0 0 0 0.07 0.15 0.13 0.06 0.09 0.18 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.21 0.21 0.35 0.12 0.06 0.07 0.05 0.07 0 0.06 0.09 0.34 0.61 0.72 0.08 0.09 0.13 0.21 0.06 0.07 0.06 0.17 0.12
Cytoplasm 0.21 0.48 0.31 0.23 0.35 0.39 0.36 0.40 0.43 0.49 0.51 0.29 0.20 0.20 0.28 0.27 0.32 0.06 0.17 0.09 0 0 0
Endoplasmic Reticulum 0.61 0.31 0.42 0.50 0.33 0.21 0.15 0.10 0.09 0.09 0.06 0.42 0.25 0.14 0 0 0 0.34 0.33 0.29 0.30 0.36 0.43
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.27 0.20 0.17 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0.06 0.10 0.14 0.16 0.10 0.07 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.18 0.25 0.32 0.42 0.43 0.44 0.37 0.44 0.06 0 0 0.33 0.32 0.22 0.32 0.35 0.40 0.56 0.39 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 21 5 5 10 3 2 5 5 2 24 72 76 11 13 29 3 1 2 6 9 11
Bud 0 2 7 0 2 4 0 5 5 3 6 11 2 4 5 3 4 0 2 0 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 5 6
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 12 14 64 30 16 32 18 20 9 18 29 113 300 404 16 13 21 18 6 4 17 84 69
Cytoplasm 12 32 57 58 87 185 133 113 134 148 168 97 97 112 56 37 50 4 17 5 5 16 10
Endoplasmic Reticulum 35 21 76 127 84 102 57 28 29 27 19 138 121 77 6 2 6 28 34 15 87 185 246
Endosome 0 0 0 0 0 1 0 0 2 0 1 0 0 0 1 1 0 0 0 0 0 0 3
Golgi 1 0 0 9 5 3 2 5 4 1 4 8 25 30 53 28 27 0 0 0 0 1 5
Mitochondria 0 5 0 0 0 0 0 2 2 3 3 1 0 3 5 7 4 0 1 2 0 2 5
Nucleus 0 0 4 14 24 69 60 28 22 12 15 3 1 1 10 6 4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Nucleolus 0 0 0 0 0 1 0 1 0 1 0 0 0 0 4 3 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 3 0 0 0 0 0 1 0 0 11 15 23 2 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 9 46 64 154 157 120 139 113 147 20 9 5 65 44 35 27 36 22 164 197 206
Unique Cell Count 57 67 183 254 251 480 373 281 314 302 332 330 489 565 197 137 157 85 104 55 292 509 574
Labelled Cell Count 63 81 241 289 287 561 430 324 352 331 394 426 642 735 234 158 181 85 104 55 292 509 574

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cell Periphery

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.8 13.8 20.0 26.0 28.7 29.1 30.1 30.3 27.6 29.4 28.5 17.1 15.4 14.3 32.4 34.3 35.9 17.8 16.8 17.8
Std Deviation (1e-4) 3.4 5.0 4.4 6.5 7.7 8.5 8.7 8.9 8.9 8.9 8.5 3.5 2.7 2.6 10.5 11.4 10.0 4.0 4.1 3.9
Intensity Change (Log2)

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole