Standard name
Description Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08
Bud 0
Bud Neck 0
Bud Site
Cell Periphery 0
Cytoplasm 0.60
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0.18
Nucleus 0
Nuclear Periphery 0
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac/Vac Membrane 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0
Bud 0
Bud Neck 0
Bud Site
Cell Periphery 0
Cytoplasm 3
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nucleus 0
Nuclear Periphery 0
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac/Vac Membrane 0
Unique Cell Count 5
Labelled Cell Count 5

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4)
Std Deviation (1e-4)
Intensity Change (Log2)

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3