Standard name
Description Ubiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC)

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.27 0.21 0.13 0.06 0 0.17 0.09 0.14 0.08 0.14 0.24 0.17 0.24 0.14 0.16 0.12 0.08 0.08 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0 0.32 0.29 0.19 0 0 0 0 0 0 0 0 0
Nucleus 0.44 0.28 0.44 0.47 0.65 0.61 0.57 0.52 0.53 0.56 0.44 0.66 0.54 0.63 0.61 0.63 0.67 0.53 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0.06 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.50 0.60 0.49 0.41 0.31 0.17 0.32 0.11 0.23 0.15 0.35 0.24 0.35 0.07 0.10 0.10 0.15 0.16 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 3 0 0 0 7 4 2 5 7 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 47 25 9 1 4 3 5 10 5 15 31 25 40 28 22 11 8 14 22
Endoplasmic Reticulum 2 2 1 0 0 0 0 0 0 0 1 1 0 1 1 0 1 1 1
Endosome 0 0 0 1 3 0 3 1 0 3 0 0 0 5 5 2 0 5 4
Golgi 2 0 0 0 1 0 0 0 0 0 1 0 0 2 0 0 0 1 0
Mitochondria 1 1 0 0 0 3 0 23 18 20 2 0 0 1 1 1 0 0 0
Nucleus 78 34 30 8 72 11 32 37 33 59 56 99 91 125 84 60 70 96 95
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 3 4 5 1 1 1 1 0 0 1 10 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 6 2 1 0 5 3
Vac/Vac Membrane 88 72 33 7 34 3 18 8 14 16 44 36 59 13 14 9 15 29 30
Unique Cell Count 176 121 68 17 111 18 56 71 62 105 127 150 169 198 139 95 106 182 178
Labelled Cell Count 218 134 73 17 114 20 58 83 74 124 136 162 191 198 139 95 106 182 178

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 10.0 11.0 14.5 12.2 7.7 10.4 6.6 6.3 7.3 12.2 11.6 11.6 11.2 11.1 11.8
Std Deviation (1e-4) 2.0 2.1 3.3 14.3 2.5 2.3 3.1 1.4 2.0 1.9 3.4 2.9 3.4 2.5 2.6 2.9
Intensity Change (Log2) 0.39 0.15 -0.52 -0.08 -0.75 -0.8 -0.6 0.14 0.07 0.07 0.03 0.0 0.09

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.7 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 5.1 4.8 3.8 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 -3.2 -2.2 -3.0 -5.0 -3.8 -5.8 -3.3 -4.0 -3.5