Standard name
Description 5-aminolevulinate synthase; catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p; has a pyridoxal phosphate cofactor whose insertion is mediated by Mcx1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Golgi 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0.2 0.45 0.38 0 0.05 0 0.09 0.11 0.09
Mitochondria 0.96 0.84 0.94 0.93 0.82 0.9 0.83 0.86 0.91 0.92 0.94 0.96 0.93 0.88 0.86 0.08 0.09 0.11 0.83 0.79 0.8 0.74 0.68 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0.07 0.05 0.05 0.09 0.1 0.11 0.07 0.09 0.07 0.07 0.05 0.05 0.06 0 0.05 0.06 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0.1 0.18 0.2 0.17 0.15 0.16 0.13 0.14 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0.05 0.08 0 0.05 0 0 0 0 0 0 0 0 0.16 0.1 0.08 0 0 0.11 0.06 0.08 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.23 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.1 0.19 0.21 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 4 0 1 0 1 0 1 0 1 0 1 1 2 1 1 1 1 8 8 3 6 11
Bud 4 0 4 4 3 9 12 11 17 14 8 7 2 1 2 5 5 9 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 1 0 0 1 0 2 4 1 1 6 0 2 0 0 1 1 0 0
Cell Periphery 4 12 13 6 3 0 1 6 2 0 1 1 5 6 4 23 23 10 0 0 2 0 0 0
Cytoplasm 3 4 3 3 7 6 7 7 7 4 3 3 5 5 9 25 16 12 0 1 0 0 1 0
Endoplasmic Reticulum 0 1 1 1 1 1 0 5 1 0 2 0 3 3 2 2 10 4 0 0 1 0 2 2
Endosome 1 4 9 7 9 2 10 7 2 0 0 0 6 6 8 14 9 9 8 10 5 4 10 6
Golgi 0 13 3 5 2 0 0 2 0 0 0 0 15 23 27 39 124 51 8 13 12 14 32 23
Mitochondria 243 201 360 299 272 514 580 714 576 649 303 335 261 280 269 16 25 15 214 213 317 127 208 180
Nucleus 1 1 0 0 1 0 1 0 5 1 0 0 0 0 3 7 7 3 0 0 0 0 1 0
Nuclear Periphery 16 17 20 17 30 57 80 57 54 50 22 17 14 20 11 9 16 3 3 2 0 1 4 2
Nucleolus 22 6 14 14 32 101 137 138 94 112 43 48 10 10 6 10 2 8 0 2 0 2 2 3
Peroxisomes 5 5 31 16 26 14 34 36 18 15 3 2 7 8 13 31 28 11 9 7 42 11 25 19
SpindlePole 0 2 2 0 5 3 7 12 5 5 1 0 1 1 0 13 16 4 1 0 2 0 3 0
Vac/Vac Membrane 26 54 8 5 13 14 10 10 10 5 7 6 25 32 32 38 58 20 7 6 1 3 4 4
Unique Cell Count 254 238 383 323 331 570 697 826 630 702 321 350 282 318 311 199 276 134 259 271 398 171 306 264
Labelled Cell Count 327 321 472 378 405 723 884 1006 793 861 394 421 355 396 389 234 340 160 259 271 398 171 306 264


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 9.7 8.9 11.0 12.5 13.9 13.9 15.6 15.1 15.3 16.5 16.2 10.6 10.5 10.1 8.0 10.5 9.9 9.1 9.1 9.6
Std Deviation (1e-4) 1.5 1.9 1.9 2.3 2.9 3.8 4.1 4.3 4.4 4.5 5.2 5.6 1.9 2.0 1.8 1.5 2.2 2.0 2.2 2.0 2.0
Intensity Change (Log2) 0.31 0.49 0.65 0.64 0.81 0.76 0.78 0.9 0.87 0.25 0.25 0.18 -0.15 0.24 0.16 0.03 0.04 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.7 0.9 0 1.8 1.2 1.5 1.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -2.2 -4.4 0 -3.5 -3.9 0 -2.9 -3.1 -1.3 -1.2 -1.8 3.9 2.8 2.0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 2.1 6.3 3.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.2 0.3 0 -1.1 -2.1 0 0 0 0 -0.2 -0.4 0.2 2.8 0.7 0
Golgi 0 0 0 0 0 0 0 0 0 3.6 4.5 5.1 8.3 14.2 12.1
Mitochondria -0.8 -4.9 -2.1 -5.0 -3.9 -1.5 -1.0 0.2 1.0 -0.7 -2.8 -3.4 -20.6 -21.8 -18.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 2.0 2.7 3.4 1.1 2.0 1.2 0.9 -0.2 -0.1 0.6 -1.1 -0.4 0.3 -1.4
Nucleolus 0.5 3.3 6.5 7.3 6.4 5.6 6.0 4.7 4.9 -0.1 -0.4 -1.4 0.8 -2.4 1.1
Peroxisomes -1.7 -0.1 -4.0 -2.1 -2.6 -3.8 -4.7 -4.4 -4.9 -3.1 -3.2 -2.1 2.8 0.9 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.3 4.1 0
Vacuole -0.5 1.5 0.4 -0.8 -1.2 -0.6 0 0.1 -0.4 4.0 4.5 4.6 7.2 8.0 5.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hem1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available