Standard name
Description Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; member of the LYR protein family; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.11 0.12 0 0 0 0 0 0
Cytoplasm 0.51 0.17 0.28 0.18 0.07 0 0 0 0 0 0 0.34 0.34 0.29 0.15 0.17 0.24 0.24 0 0.07 0.09 0 0
Endoplasmic Reticulum 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0.09 0 0 0 0 0 0 0.14 0.16 0.14 0.1 0.1 0.13 0 0 0.09 0.05 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.22 0.21 0.12 0 0 0 0 0 0
Mitochondria 0.55 0.72 0.73 0.86 0.83 0.94 0.93 0.94 0.95 0.97 0.98 0.43 0.39 0.32 0.09 0.19 0.13 0.5 0.72 0.67 0.64 0.6 0.61
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.06 0.07 0.12 0.1 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0.08 0.09 0 0.12 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0.07 0.06 0 0.06 0 0 0 0.15 0.15 0.18 0.22 0.14 0.11 0.09 0 0 0.06 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 4 0 0 0 0 0 0 1 0 1 2 1 1 5 3 4 4 5 4 9 24
Bud 0 2 2 8 10 5 13 11 10 9 14 2 5 2 1 2 3 1 1 0 2 7 10
Bud Neck 2 0 2 0 0 0 0 0 0 0 0 1 1 0 0 0 0 3 0 1 1 9 10
Bud Site 0 0 0 0 0 2 7 3 6 4 9 0 0 0 0 0 1
Cell Periphery 0 2 2 6 4 1 1 2 2 5 7 8 9 11 14 10 11 1 0 0 0 0 0
Cytoplasm 89 9 49 37 17 7 16 7 2 7 3 92 116 118 13 16 22 50 2 14 17 17 5
Endoplasmic Reticulum 3 0 14 2 3 2 3 0 6 4 9 7 6 8 4 2 4 2 1 3 1 2 4
Endosome 0 3 0 1 20 1 2 0 1 0 0 37 54 56 9 9 12 6 2 20 10 31 32
Golgi 0 0 0 1 1 1 0 0 1 0 0 11 12 37 19 20 11 1 2 0 5 9 17
Mitochondria 96 39 128 177 189 280 456 323 360 282 387 115 133 132 8 18 12 105 62 149 126 233 273
Nucleus 0 0 0 1 1 3 5 1 4 0 2 0 1 1 4 2 1 0 0 0 1 3 1
Nuclear Periphery 0 0 0 0 3 17 32 41 38 20 34 1 1 1 0 0 1 0 0 1 1 2 1
Nucleolus 0 1 0 0 1 16 21 22 27 13 14 1 0 0 0 0 1 1 0 0 1 2 0
Peroxisomes 0 3 0 3 12 4 9 1 0 1 0 8 10 31 8 4 11 0 2 5 2 7 9
SpindlePole 1 1 2 2 7 5 5 3 6 4 3 6 5 6 3 8 6 5 1 4 4 9 17
Vac/Vac Membrane 9 2 3 0 17 17 19 21 16 8 8 40 50 74 19 13 10 18 2 9 12 35 32
Unique Cell Count 174 54 175 206 229 297 490 345 377 291 394 268 344 409 87 94 92 210 87 224 199 390 449
Labelled Cell Count 200 62 206 238 285 361 589 435 479 358 490 330 405 478 103 109 109 210 87 224 199 390 449


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 5.8 3.4 3.9 5.0 4.5 4.9 4.6 4.8 4.8 4.8 5.1 5.5 5.7 7.1 6.7 6.8 5.2 5.3 5.5
Std Deviation (1e-4) 0.6 2.1 0.6 2.2 1.8 1.5 1.5 1.6 1.4 1.6 1.5 1.3 1.5 1.5 1.4 1.6 2.0 2.0 1.5 1.5
Intensity Change (Log2) 0.19 0.56 0.4 0.52 0.44 0.51 0.49 0.48 0.59 0.7 0.75 1.06 0.98 1.0 0.62 0.64 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1.9 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 4.8 0 0
Cytoplasm -2.3 -5.5 -8.3 -9.5 -9.0 -10.4 -8.2 -10.4 1.4 1.3 0.2 -2.3 -2.0 -0.7
Endoplasmic Reticulum 0 -3.3 0 0 0 0 0 0 -2.6 -3.5 -3.5 -1.0 -1.9 -1.1
Endosome 0 4.0 0 0 0 0 0 0 5.1 5.5 5.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 4.1 6.4 6.3 0
Mitochondria 0 2.3 0 6.9 0 0 0 0 -6.3 -7.4 -9.1 -9.8 -8.5 -9.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 3.5 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.8 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 3.1 0 0 0 0 0 0 0 0 3.7 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.6 2.1 1.4 2.2 1.5 0 0 4.6 4.6 5.4 5.5 4.0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sdh6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available