Standard name
Description Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.08 0.05 0 0.07 0 0.11 0.14 0.15 0.12 0.15 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.08 0.05 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.96 0.97 0.91 0.96 0.91 0.9 0.86 0.86 0.83 0.77 0.77 0.86 0.84 0.79 0.75 0.74 0.76 0.97 0.95 0.93 0.92 0.82 0.75
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0 0.08 0.1 0.08 0.11 0.14 0.24 0.29 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.11 0 0 0 0 0.1 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 1 0 0 0 4 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 0 0 1 1 3 1 4 0 2 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 10 2 4 7 5 12 20 9 7 10 3 13 13 26 14 16 10 0 1 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 0 0 0 0 0 0
Endosome 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 1 1 0 0 0 1 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Mitochondria 4 11 0 2 0 3 3 5 14 11 24 10 2 0 1 3 2 3 0 0 0 0 0 0
Nucleus 135 343 152 105 211 191 246 227 146 172 114 74 105 79 135 85 78 77 135 335 161 69 153 137
Nuclear Periphery 0 2 0 0 1 0 1 1 2 1 1 0 0 0 1 1 2 1 0 0 0 0 0 0
Nucleolus 1 0 1 6 4 16 26 22 18 29 35 28 0 0 2 4 4 2 0 0 0 1 3 8
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 2 1 0 0 0 2 0 6 0 0 0 0 0 1 0 2 0
Vac/Vac Membrane 1 3 3 0 3 4 1 3 0 4 0 0 1 2 1 9 6 11 0 7 3 2 19 28
Unique Cell Count 139 359 157 115 220 210 273 264 169 208 148 96 122 94 170 114 105 101 140 353 174 75 188 182
Labelled Cell Count 142 370 160 117 226 220 290 285 195 225 188 115 127 95 174 120 111 107 140 353 174 75 188 182


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 8.5 10.0 8.3 9.5 8.6 8.2 8.5 7.5 7.6 6.9 6.8 10.1 10.7 10.0 14.2 15.8 13.0 8.7 10.6 11.2
Std Deviation (1e-4) 1.8 2.2 2.0 2.2 1.7 2.0 2.0 2.1 2.3 2.1 2.0 2.1 3.3 3.1 3.2 5.0 7.6 4.7 1.3 2.3 2.4
Intensity Change (Log2) -0.27 -0.08 -0.23 -0.28 -0.24 -0.42 -0.41 -0.53 -0.56 0.0 0.1 -0.0 0.5 0.66 0.37 -0.21 0.08 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 1.8 2.8 2.0 0 2.5 0 3.4 4.1 4.5 3.8 4.4 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.7 0 5.3 0 0 0 0 0 0 0
Nucleus -2.0 -0.5 -2.2 -2.6 -3.6 -3.4 -4.2 -5.2 -4.9 -3.3 -3.6 -4.8 -5.5 -5.5 -5.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 3.7 3.4 3.9 4.6 6.2 6.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 3.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uba2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available