Standard name
Description Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.09 0.08 0.07 0.07 0.06 0.05 0.06 0.06 0 0.09 0.15 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.96 0.93 0.92 0.88 0.82 0.82 0.81 0.8 0.77 0.77 0.75 0.94 0.87 0.82 0.93 0.9 0.88 0.94 0.95 0.91 0.84 0.73 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.15 0.13 0.17 0.24 0.26 0.3 0.3 0 0 0.05 0.05 0 0.06 0 0 0 0.07 0.13 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0.06 0.12 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 1 2 1 1 3
Bud Neck 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2 1 2 1 1 2 4 0 4 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 6 8 13 20 20 29 39 19 18 22 18 9 34 63 7 9 7 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Endosome 1 0 0 0 1 1 3 0 0 0 0 1 0 1 0 0 1 3 2 2 0 0 0 2
Golgi 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 0 0 0 1 0 14 0 7 7 11 7 11 1 0 1 1 4 2 0 0 0 1 0 4
Nucleus 170 206 157 222 201 204 328 425 267 292 274 239 211 312 350 221 256 292 164 206 162 244 288 244
Nuclear Periphery 0 1 0 0 0 1 1 0 3 2 2 1 0 1 0 0 4 2 0 0 0 0 0 2
Nucleolus 8 4 4 9 6 38 53 89 79 100 107 96 6 13 23 13 11 21 4 2 3 21 52 56
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 0 0 1 0 0 0 10 4 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 2 4 1 1 4 2 6 1 3 4 4 0 3 2 5 14 22 2 3 4 16 47 74
Unique Cell Count 179 215 169 242 228 249 399 527 332 379 354 317 224 359 426 238 285 331 175 217 178 290 396 391
Labelled Cell Count 183 219 173 247 230 285 418 570 378 431 419 374 227 382 448 248 300 350 175 217 178 290 396 391


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.9 20.6 16.8 15.9 16.0 10.7 13.4 13.5 12.6 12.8 12.2 13.0 16.4 16.0 19.1 18.0 18.1 20.4 16.9 17.9 18.8
Std Deviation (1e-4) 4.8 7.5 6.2 5.4 6.2 3.3 3.8 3.7 3.6 3.9 4.2 5.0 6.0 7.7 8.3 7.7 9.0 9.6 5.9 5.5 6.1
Intensity Change (Log2) -0.07 -0.07 -0.64 -0.32 -0.31 -0.41 -0.39 -0.46 -0.36 -0.03 -0.07 0.19 0.1 0.11 0.28 0.01 0.1 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 1.6 1.3 1.1 1.2 0.5 0 0.7 0.4 -0.3 1.9 3.4 -0.9 -0.9 -1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.4 -1.6 -3.2 -3.3 -3.7 -3.7 -4.5 -4.4 -4.7 0.5 -2.0 -3.3 0 -1.1 -1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 0 4.3 4.0 4.8 6.1 6.6 7.3 7.3 0 0.8 1.6 1.5 0.9 1.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.3 2.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ncb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available