Standard name
Human Ortholog
Description Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.69 0.76 0.82 0.84 0.88 0.85 0.88 0.83 0.82 0.83 0.79 0.63 0.53 0.57 0.87 0.86 0.84 0.21 0.27 0.11 0.11 0.13 0.14
Endoplasmic Reticulum – 0.27 0.22 0.19 0.11 0.05 0 0 0 0 0 0.05 0.37 0.47 0.46 0 0 0.05 0.43 0.44 0.64 0.52 0.47 0.41
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0.07 0.07
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0.09 0.06 0.12 0.11 0.18 0.21 0 0 0 0.05 0 0 0.12 0 0.06 0.05 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0.05 0.09 0.07 0.13 0.16 0.13 0.21 0.21 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 2 2 1 3 2 2 4 6 2 0 8 3 19 9 6 6 5 0 2 3 4 10 7
Bud – 2 2 1 3 14 17 22 15 16 20 24 0 3 2 1 1 0 0 0 0 3 5 6
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 3 5
Bud Site – 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 5 1 6 4 1 5 2 5 7 8 15 3 4 0 0 0 0 0 0 0
Cytoplasm – 86 110 163 305 263 453 523 338 443 254 379 181 336 362 239 182 147 5 39 19 33 64 84
Endoplasmic Reticulum – 34 32 37 40 16 14 19 11 18 8 25 105 299 291 10 4 9 11 65 113 163 239 251
Endosome – 3 0 4 13 4 9 8 11 13 1 3 0 5 1 5 8 11 0 3 3 15 37 45
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 7 7
Mitochondria – 1 2 1 5 7 47 35 47 58 56 99 0 0 0 13 6 1 3 5 10 17 21 17
Nucleus – 1 0 0 4 0 1 3 0 2 0 1 2 0 2 0 3 1 0 0 0 0 1 1
Nuclear Periphery – 0 0 0 0 1 2 2 0 1 0 0 0 1 0 0 0 0 0 1 0 2 3 5
Nucleolus – 1 0 0 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1 0 1 0 1 0 0 0 1
Vac/Vac Membrane – 1 1 1 9 10 27 18 26 22 10 12 2 5 3 13 20 12 3 23 23 67 107 181
Unique Cell Count 125 144 198 363 298 533 594 408 538 306 480 287 632 637 275 212 174 28 148 178 317 509 620
Labelled Cell Count 131 149 208 387 319 579 639 456 585 351 556 300 676 685 291 234 188 28 148 178 317 509 620


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 7.2 5.9 6.6 6.4 5.8 5.0 5.2 5.1 4.9 4.7 4.6 8.9 8.1 8.3 6.6 6.8 7.3 9.6 10.0 10.7
Std Deviation (1e-4) – 1.1 0.7 1.0 1.1 1.1 1.1 1.0 1.0 0.9 0.8 0.8 1.1 1.0 1.0 1.1 1.1 1.2 1.4 1.6 1.5
Intensity Change (Log2) – – – 0.16 0.1 -0.03 -0.24 -0.2 -0.23 -0.29 -0.34 -0.36 0.58 0.45 0.49 0.16 0.2 0.3 0.7 0.76 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.7 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 2.0 3.2 2.4 3.6 1.7 1.6 1.7 0.7 -2.8 -5.1 -4.3 2.7 2.3 1.8
Endoplasmic Reticulum -0.8 -3.3 -5.3 -8.3 -8.1 -7.5 0 0 0 3.0 5.5 5.2 -6.0 -6.2 -4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 2.2 3.7 0 0 0 0 0 0 1.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.3 0 2.7 0 0 0 0 0 0 0 3.4 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2418 6.3151 5.4903 5.6034 5.1918 5.5403 4.578 4.9851 4.7181 4.3072 4.1472 4.8338 3.3494 4.3876 3.9317 4.1968 2.983 3.7183
Actin 0.0207 0.0009 0.0027 0.01 0.0026 0.0024 0.004 0.0015 0.0028 0.0504 0.0002 0.0006 0.0033 0.0059 0.0012 0.0155 0.0022 0.0091
Bud 0.0024 0.0002 0.0003 0.0003 0.0002 0.0007 0.0002 0.0004 0.0007 0.0026 0.0002 0.0002 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001
Bud Neck 0.0012 0.0009 0.0005 0.0012 0.0008 0.0013 0.0003 0.0003 0.0005 0.0012 0.0002 0.0005 0.0004 0.0015 0.0006 0.001 0.0004 0.0006
Bud Periphery 0.0018 0.0003 0.0003 0.0005 0.0002 0.0005 0.0003 0.0005 0.0007 0.0022 0.0003 0.0003 0.0005 0.0001 0.0002 0.0004 0.0003 0.0001
Bud Site 0.0037 0.0026 0.0006 0.002 0.0003 0.0003 0.0009 0.0017 0.001 0.0044 0.0002 0.0001 0.0006 0.0003 0.0005 0.0005 0.0004 0.0001
Cell Periphery 0.0005 0.001 0.0003 0.0003 0.0002 0.0002 0.001 0.002 0.0006 0.0008 0.0002 0.0004 0.0007 0.0004 0.0003 0.0004 0.0002 0.0001
Cytoplasm 0.2267 0.4214 0.2443 0.2486 0.3173 0.3356 0.1695 0.3085 0.1758 0.2563 0.1814 0.2558 0.1616 0.2885 0.1999 0.1735 0.2795 0.2062
Cytoplasmic Foci 0.0432 0.0287 0.0386 0.0197 0.0539 0.0347 0.0401 0.0316 0.0365 0.0347 0.0361 0.04 0.0282 0.0182 0.0287 0.0209 0.0164 0.0238
Eisosomes 0.0004 0 0 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0009 0 0 0 0.0001 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.3085 0.2978 0.3208 0.3928 0.1693 0.3498 0.2914 0.3103 0.2613 0.2809 0.1679 0.2642 0.3685 0.4247 0.3705 0.1975 0.2428 0.3571
Endosome 0.1965 0.1594 0.2375 0.117 0.2612 0.1568 0.2968 0.1912 0.3388 0.2051 0.4316 0.3151 0.2514 0.1481 0.2586 0.2513 0.2444 0.2852
Golgi 0.0132 0.0056 0.0179 0.0071 0.0111 0.0076 0.0234 0.015 0.0265 0.0166 0.0122 0.0221 0.0172 0.0104 0.0156 0.0208 0.0081 0.0095
Lipid Particles 0.0233 0.0129 0.0132 0.0213 0.0076 0.0085 0.0282 0.0106 0.0147 0.0086 0.0025 0.0088 0.0142 0.0074 0.0128 0.0194 0.0027 0.0076
Mitochondria 0.0129 0.0028 0.0133 0.0046 0.0047 0.0063 0.0089 0.0103 0.0101 0.0172 0.0085 0.0066 0.0047 0.001 0.0022 0.0028 0.0396 0.0027
None 0.0128 0.0048 0.0066 0.0042 0.0256 0.0146 0.0321 0.0106 0.0248 0.0229 0.0381 0.0149 0.0034 0.0032 0.0052 0.0028 0.0009 0.0082
Nuclear Periphery 0.0431 0.0176 0.0287 0.0312 0.0267 0.0258 0.0258 0.0172 0.0199 0.0182 0.0173 0.013 0.0514 0.0353 0.0373 0.0537 0.0275 0.0323
Nucleolus 0.0013 0.0001 0.0002 0.0006 0.0023 0.0007 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0 0.0002 0.0001 0.0001
Nucleus 0.0093 0.0045 0.0044 0.0053 0.016 0.0077 0.0026 0.0029 0.0019 0.0021 0.0021 0.0019 0.0044 0.0036 0.0031 0.012 0.0051 0.0038
Peroxisomes 0.0138 0.0002 0.0035 0.0183 0.0015 0.0009 0.0027 0.0002 0.0022 0.0049 0.0003 0.0003 0.0008 0.0002 0.0003 0.0018 0.0006 0.0002
Punctate Nuclear 0.0062 0.0009 0.0024 0.0026 0.0223 0.0027 0.0017 0.0005 0.0016 0.001 0.0005 0.0005 0.0024 0.0022 0.0006 0.0026 0.0004 0.001
Vacuole 0.0507 0.0335 0.0546 0.1032 0.067 0.037 0.0584 0.0779 0.064 0.0529 0.0731 0.0444 0.0749 0.0439 0.052 0.1918 0.112 0.0396
Vacuole Periphery 0.0079 0.0038 0.0094 0.009 0.0094 0.0058 0.0111 0.0064 0.0155 0.0157 0.0269 0.0104 0.011 0.0049 0.0102 0.0306 0.0162 0.0125

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.3514 27.3993 18.2983 26.848 20.8631 13.7849 24.9331 28.0469 24.8885 31.3681
Translational Efficiency 0.8327 0.6288 0.944 0.5529 0.7827 1.9183 0.8823 0.4876 0.8732 0.6379

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
Localization
Cell Percentages ER (80%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ste14

Ste14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available