Standard name
Description Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.69 0.76 0.82 0.84 0.88 0.85 0.88 0.83 0.82 0.83 0.79 0.63 0.53 0.57 0.87 0.86 0.84 0.21 0.27 0.11 0.11 0.13 0.14
Endoplasmic Reticulum – 0.27 0.22 0.19 0.11 0.05 0 0 0 0 0 0.05 0.37 0.47 0.46 0 0 0.05 0.43 0.44 0.64 0.52 0.47 0.41
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0.07 0.07
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0.09 0.06 0.12 0.11 0.18 0.21 0 0 0 0.05 0 0 0.12 0 0.06 0.05 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0.05 0.09 0.07 0.13 0.16 0.13 0.21 0.21 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 2 2 1 3 2 2 4 6 2 0 8 3 19 9 6 6 5 0 2 3 4 10 7
Bud – 2 2 1 3 14 17 22 15 16 20 24 0 3 2 1 1 0 0 0 0 3 5 6
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 3 5
Bud Site – 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 5 1 6 4 1 5 2 5 7 8 15 3 4 0 0 0 0 0 0 0
Cytoplasm – 86 110 163 305 263 453 523 338 443 254 379 181 336 362 239 182 147 5 39 19 33 64 84
Endoplasmic Reticulum – 34 32 37 40 16 14 19 11 18 8 25 105 299 291 10 4 9 11 65 113 163 239 251
Endosome – 3 0 4 13 4 9 8 11 13 1 3 0 5 1 5 8 11 0 3 3 15 37 45
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 7 7
Mitochondria – 1 2 1 5 7 47 35 47 58 56 99 0 0 0 13 6 1 3 5 10 17 21 17
Nucleus – 1 0 0 4 0 1 3 0 2 0 1 2 0 2 0 3 1 0 0 0 0 1 1
Nuclear Periphery – 0 0 0 0 1 2 2 0 1 0 0 0 1 0 0 0 0 0 1 0 2 3 5
Nucleolus – 1 0 0 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1 0 1 0 1 0 0 0 1
Vac/Vac Membrane – 1 1 1 9 10 27 18 26 22 10 12 2 5 3 13 20 12 3 23 23 67 107 181
Unique Cell Count 125 144 198 363 298 533 594 408 538 306 480 287 632 637 275 212 174 28 148 178 317 509 620
Labelled Cell Count 131 149 208 387 319 579 639 456 585 351 556 300 676 685 291 234 188 28 148 178 317 509 620


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 7.2 5.9 6.6 6.4 5.8 5.0 5.2 5.1 4.9 4.7 4.6 8.9 8.1 8.3 6.6 6.8 7.3 9.6 10.0 10.7
Std Deviation (1e-4) – 1.1 0.7 1.0 1.1 1.1 1.1 1.0 1.0 0.9 0.8 0.8 1.1 1.0 1.0 1.1 1.1 1.2 1.4 1.6 1.5
Intensity Change (Log2) – – – 0.16 0.1 -0.03 -0.24 -0.2 -0.23 -0.29 -0.34 -0.36 0.58 0.45 0.49 0.16 0.2 0.3 0.7 0.76 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.7 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 2.0 3.2 2.4 3.6 1.7 1.6 1.7 0.7 -2.8 -5.1 -4.3 2.7 2.3 1.8
Endoplasmic Reticulum -0.8 -3.3 -5.3 -8.3 -8.1 -7.5 0 0 0 3.0 5.5 5.2 -6.0 -6.2 -4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 2.2 3.7 0 0 0 0 0 0 1.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.3 0 2.7 0 0 0 0 0 0 0 3.4 2.8