Standard name | |
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Description | Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 2 | 2 | 1 | 3 | 2 | 2 | 4 | 6 | 2 | 0 | 8 | 3 | 19 | 9 | 6 | 6 | 5 | 0 | 2 | 3 | 4 | 10 | 7 |
Bud | – | 2 | 2 | 1 | 3 | 14 | 17 | 22 | 15 | 16 | 20 | 24 | 0 | 3 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 5 | 6 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | 5 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 5 | 1 | 6 | 4 | 1 | 5 | 2 | 5 | 7 | 8 | 15 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 86 | 110 | 163 | 305 | 263 | 453 | 523 | 338 | 443 | 254 | 379 | 181 | 336 | 362 | 239 | 182 | 147 | 5 | 39 | 19 | 33 | 64 | 84 |
Endoplasmic Reticulum | – | 34 | 32 | 37 | 40 | 16 | 14 | 19 | 11 | 18 | 8 | 25 | 105 | 299 | 291 | 10 | 4 | 9 | 11 | 65 | 113 | 163 | 239 | 251 |
Endosome | – | 3 | 0 | 4 | 13 | 4 | 9 | 8 | 11 | 13 | 1 | 3 | 0 | 5 | 1 | 5 | 8 | 11 | 0 | 3 | 3 | 15 | 37 | 45 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 7 | 7 |
Mitochondria | – | 1 | 2 | 1 | 5 | 7 | 47 | 35 | 47 | 58 | 56 | 99 | 0 | 0 | 0 | 13 | 6 | 1 | 3 | 5 | 10 | 17 | 21 | 17 |
Nucleus | – | 1 | 0 | 0 | 4 | 0 | 1 | 3 | 0 | 2 | 0 | 1 | 2 | 0 | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 5 |
Nucleolus | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | – | 1 | 1 | 1 | 9 | 10 | 27 | 18 | 26 | 22 | 10 | 12 | 2 | 5 | 3 | 13 | 20 | 12 | 3 | 23 | 23 | 67 | 107 | 181 |
Unique Cell Count | 125 | 144 | 198 | 363 | 298 | 533 | 594 | 408 | 538 | 306 | 480 | 287 | 632 | 637 | 275 | 212 | 174 | 28 | 148 | 178 | 317 | 509 | 620 | |
Labelled Cell Count | 131 | 149 | 208 | 387 | 319 | 579 | 639 | 456 | 585 | 351 | 556 | 300 | 676 | 685 | 291 | 234 | 188 | 28 | 148 | 178 | 317 | 509 | 620 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 7.2 | 5.9 | 6.6 | 6.4 | 5.8 | 5.0 | 5.2 | 5.1 | 4.9 | 4.7 | 4.6 | 8.9 | 8.1 | 8.3 | 6.6 | 6.8 | 7.3 | 9.6 | 10.0 | 10.7 |
Std Deviation (1e-4) | – | 1.1 | 0.7 | 1.0 | 1.1 | 1.1 | 1.1 | 1.0 | 1.0 | 0.9 | 0.8 | 0.8 | 1.1 | 1.0 | 1.0 | 1.1 | 1.1 | 1.2 | 1.4 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.1 | -0.03 | -0.24 | -0.2 | -0.23 | -0.29 | -0.34 | -0.36 | 0.58 | 0.45 | 0.49 | 0.16 | 0.2 | 0.3 | 0.7 | 0.76 | 0.85 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 2.0 | 3.2 | 2.4 | 3.6 | 1.7 | 1.6 | 1.7 | 0.7 | -2.8 | -5.1 | -4.3 | 2.7 | 2.3 | 1.8 |
Endoplasmic Reticulum | -0.8 | -3.3 | -5.3 | -8.3 | -8.1 | -7.5 | 0 | 0 | 0 | 3.0 | 5.5 | 5.2 | -6.0 | -6.2 | -4.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.1 | 2.2 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.3 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 2.8 |