Standard name
Description Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 1.0 0.99 0.98 – 0.81 0.83 0.71 0.72 0.64 0.99 0.99 0.98 0.98 0.97 0.94 0.95 0.92 0.89 0.89 0.89 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.11 0.09 0.18 0.09 0.15 0 0 0 0.05 0.06 0.05 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0.05 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0.06 0.05 0.14 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 2 3 2 4 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 – 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 1 0 – 1 3 10 6 1 1 0 3 4 2 9 0 0 1 0 0 0
Cytoplasm 198 292 121 58 92 65 – 85 73 60 50 30 231 226 254 337 334 399 180 278 117 181 280 299
Endoplasmic Reticulum 0 3 0 0 1 0 – 12 8 15 6 7 5 2 5 18 22 23 0 5 6 5 13 9
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 5 2 2 3 5 0 0 1 0 1 3 0 0 0 0 1 1
Mitochondria 0 0 0 0 0 0 – 2 5 4 10 8 0 0 0 0 5 12 0 0 0 1 1 0
Nucleus 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 – 0 0 0 0 0 0 1 0 1 3 9 1 5 0 5 5 5
Unique Cell Count 198 296 122 58 93 66 105 88 85 69 47 233 229 260 344 346 425 191 301 132 203 317 331
Labelled Cell Count 198 296 122 58 94 66 109 94 93 80 53 237 229 264 360 368 457 191 301 132 203 317 331


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 221.9 253.4 118.6 134.0 114.5 103.4 – 83.8 71.4 72.6 68.6 62.9 191.3 180.5 170.8 228.5 219.3 216.0 190.5 195.6 204.6
Std Deviation (1e-4) 52.3 64.5 39.3 38.0 38.6 37.9 – 27.7 22.7 22.2 23.8 20.3 54.9 62.3 54.2 77.4 71.5 76.9 67.5 70.6 73.4
Intensity Change (Log2) – – – 0.18 -0.05 -0.2 – -0.5 -0.73 -0.71 -0.79 -0.92 0.69 0.61 0.53 0.95 0.89 0.86 0.68 0.72 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 -0.2 -0.4 – -4.7 -4.4 -6.1 -5.8 -6.7 0 -0.4 -1.0 -0.9 -1.5 -2.4
Endoplasmic Reticulum 0 0 0 – 3.8 0 4.8 0 0 0 0 0 0 2.9 2.6
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0