Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 1.0 0.99 0.98 0.81 0.83 0.71 0.72 0.64 0.99 0.99 0.98 0.98 0.97 0.94 0.95 0.92 0.89 0.89 0.89 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 0.09 0.18 0.09 0.15 0 0 0 0.05 0.06 0.05 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.05 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0.05 0.14 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 3 2 4 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 1 0 1 3 10 6 1 1 0 3 4 2 9 0 0 1 0 0 0
Cytoplasm 198 292 121 58 92 65 85 73 60 50 30 231 226 254 337 334 399 180 278 117 181 280 299
Endoplasmic Reticulum 0 3 0 0 1 0 12 8 15 6 7 5 2 5 18 22 23 0 5 6 5 13 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 5 2 2 3 5 0 0 1 0 1 3 0 0 0 0 1 1
Mitochondria 0 0 0 0 0 0 2 5 4 10 8 0 0 0 0 5 12 0 0 0 1 1 0
Nucleus 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 3 9 1 5 0 5 5 5
Unique Cell Count 198 296 122 58 93 66 105 88 85 69 47 233 229 260 344 346 425 191 301 132 203 317 331
Labelled Cell Count 198 296 122 58 94 66 109 94 93 80 53 237 229 264 360 368 457 191 301 132 203 317 331


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 221.9 253.4 118.6 134.0 114.5 103.4 83.8 71.4 72.6 68.6 62.9 191.3 180.5 170.8 228.5 219.3 216.0 190.5 195.6 204.6
Std Deviation (1e-4) 52.3 64.5 39.3 38.0 38.6 37.9 27.7 22.7 22.2 23.8 20.3 54.9 62.3 54.2 77.4 71.5 76.9 67.5 70.6 73.4
Intensity Change (Log2) 0.18 -0.05 -0.2 -0.5 -0.73 -0.71 -0.79 -0.92 0.69 0.61 0.53 0.95 0.89 0.86 0.68 0.72 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 -0.2 -0.4 -4.7 -4.4 -6.1 -5.8 -6.7 0 -0.4 -1.0 -0.9 -1.5 -2.4
Endoplasmic Reticulum 0 0 0 3.8 0 4.8 0 0 0 0 0 0 2.9 2.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 227.9761 294.6392 259.552 290.7605 240.0931 263.6969 200.4166 270.073 231.7443 262.815 226.7108 216.0426 171.8682 315.3212 269.5141 225.5476 153.1848 244.6882
Actin 0.0127 0.0012 0.0119 0.0008 0.0006 0.0343 0.0135 0.0008 0.0107 0.0008 0.0017 0.0054 0.0171 0.0012 0.0055 0.0013 0.0111 0.0065
Bud 0.007 0.0039 0.0079 0.0027 0.0033 0.0086 0.0055 0.0037 0.0051 0.0034 0.0064 0.0163 0.0056 0.0032 0.0084 0.0035 0.0115 0.0191
Bud Neck 0.0013 0.0017 0.0018 0.0013 0.0022 0.006 0.0015 0.0009 0.0018 0.001 0.0012 0.0026 0.0011 0.0007 0.0018 0.0015 0.0026 0.0031
Bud Periphery 0.0081 0.0024 0.0068 0.002 0.0015 0.0075 0.0078 0.0026 0.0045 0.0018 0.0057 0.0347 0.0071 0.0019 0.008 0.0028 0.0278 0.0185
Bud Site 0.0036 0.003 0.0048 0.0008 0.0004 0.0019 0.0031 0.002 0.0026 0.0005 0.0007 0.0015 0.0035 0.0031 0.0035 0.0021 0.0014 0.0038
Cell Periphery 0.0774 0.0617 0.0535 0.1009 0.0339 0.035 0.0865 0.0505 0.0465 0.0641 0.0169 0.0163 0.0663 0.0271 0.0537 0.0506 0.0155 0.0552
Cytoplasm 0.3839 0.6335 0.5427 0.2026 0.5403 0.5366 0.398 0.7288 0.5023 0.3425 0.5455 0.3589 0.3655 0.7797 0.6353 0.3267 0.4126 0.5896
Cytoplasmic Foci 0.0068 0.0013 0.0109 0.001 0.0039 0.0106 0.0084 0.0013 0.0062 0.0015 0.0022 0.0072 0.0115 0.0019 0.0046 0.0135 0.02 0.0023
Eisosomes 0.0023 0.0005 0.0008 0.0001 0.0015 0.002 0.0019 0 0.0006 0 0.0001 0.0001 0.0036 0.0004 0.0006 0.0011 0.0007 0.0004
Endoplasmic Reticulum 0.0394 0.0136 0.0245 0.0014 0.018 0.0241 0.0364 0.0066 0.0101 0.0017 0.002 0.0149 0.0355 0.0068 0.0055 0.0103 0.0044 0.0053
Endosome 0.0086 0.0035 0.0077 0.0036 0.0133 0.0273 0.008 0.0022 0.0154 0.0062 0.0099 0.0375 0.0243 0.0031 0.0074 0.0175 0.0131 0.0065
Golgi 0.0025 0.0005 0.004 0.0003 0.0009 0.024 0.0068 0.0004 0.0097 0.0008 0.0005 0.0067 0.0043 0.0005 0.0022 0.0022 0.0047 0.0022
Lipid Particles 0.0034 0.0012 0.0025 0.0035 0.0107 0.0065 0.0035 0.0013 0.0093 0.0015 0.0012 0.0025 0.0091 0.0014 0.0034 0.0193 0.0037 0.0066
Mitochondria 0.0606 0.0113 0.0213 0.0028 0.0039 0.0459 0.0366 0.0034 0.0132 0.0029 0.0162 0.0477 0.0588 0.0124 0.0144 0.0144 0.0804 0.0833
None 0.0106 0.0009 0.0071 0.0007 0.0094 0.0056 0.0055 0.0007 0.0029 0.0009 0.0008 0.0022 0.0181 0.002 0.0043 0.023 0.0423 0.0028
Nuclear Periphery 0.0573 0.0148 0.014 0.0279 0.0145 0.0301 0.0651 0.0052 0.0125 0.0082 0.0223 0.0246 0.1048 0.01 0.0156 0.0301 0.0261 0.0357
Nucleolus 0.0087 0.0029 0.0072 0.015 0.0031 0.0073 0.0087 0.0015 0.0122 0.0053 0.0026 0.0066 0.021 0.0071 0.008 0.0363 0.0089 0.004
Nucleus 0.0324 0.0178 0.0169 0.0269 0.0146 0.0146 0.0348 0.006 0.0122 0.0166 0.0144 0.0586 0.0343 0.0117 0.0183 0.0385 0.0379 0.0311
Peroxisomes 0.0006 0.0003 0.0008 0.0003 0.0003 0.0021 0.0006 0.0002 0.0009 0.0002 0.0002 0.0013 0.0012 0.0003 0.0006 0.001 0.0016 0.0006
Punctate Nuclear 0.0004 0.0001 0.0009 0.0001 0.0002 0.0004 0.0006 0 0.0004 0.0001 0.0001 0.0006 0.0028 0.0001 0.0003 0.0021 0.0006 0.0003
Vacuole 0.1924 0.1854 0.1862 0.3624 0.2231 0.0972 0.207 0.16 0.2573 0.4147 0.2255 0.2125 0.1383 0.104 0.1588 0.2844 0.1924 0.0827
Vacuole Periphery 0.08 0.0386 0.0657 0.2429 0.1003 0.0724 0.0602 0.0219 0.0637 0.1253 0.1238 0.1414 0.0662 0.0215 0.0397 0.1177 0.0807 0.0406

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2512.5716 2606.5684 3105.574 2528.541 2723.3339 2347.3117 2363.6377 2146.4353 2333.624 2204.555
Translational Efficiency 1.4016 1.3947 1.2056 1.4002 1.5806 1.5314 1.3839 1.4737 1.4159 1.481

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl12b

Rpl12b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl12b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available