Standard name
Human Ortholog
Description Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.96 1.0 0.98 0.99 0.97 0.95 0.96 0.9 0.86 0.77 0.91 0.96 1.0 0.99 0.95 0.95 0.96 0.9 0.9 0.89 0.76 0.83 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0 0.06 0.11 0.05 0.24 0.27 0.37 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0.09 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 1
Bud 1 0 0 1 1 0 2 1 3 12 16 4 4 0 4 0 0 0 0 1 4 2 2 3
Bud Neck 0 0 0 0 0 1 1 2 0 0 2 1 0 0 0 0 0 0 0 0 0 1 1 5
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4 2 4 4 3 2 6 3 5 7 1 1 2 3 7 7 17 14 0 0 0 1 0 0
Cytoplasm 169 135 241 251 317 259 378 307 301 300 196 297 230 321 412 265 237 206 159 138 204 140 242 210
Endoplasmic Reticulum 1 0 1 1 0 0 0 0 0 1 1 0 2 1 1 12 12 8 2 0 2 9 11 9
Endosome 0 0 0 1 0 0 1 0 2 2 2 3 2 0 0 1 0 1 1 3 0 2 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0
Mitochondria 3 11 0 0 2 16 43 16 79 96 94 49 2 2 1 6 1 3 2 1 5 6 9 7
Nucleus 1 0 1 1 0 3 3 8 4 2 2 1 0 0 1 4 3 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 1 2 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 2 2 1 3 1 3 1 2 1 0 13 15 5 2 2 4 16 15 15
Unique Cell Count 173 141 242 255 320 267 398 320 334 350 254 328 240 322 416 278 250 215 177 153 229 184 291 261
Labelled Cell Count 179 149 247 260 327 283 436 339 399 422 318 358 245 328 428 308 287 239 177 153 229 184 291 261


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.4 6.1 5.6 5.9 4.9 5.0 5.5 4.4 4.4 4.3 4.8 5.9 6.0 6.1 8.8 8.8 8.6 5.5 5.5 6.0
Std Deviation (1e-4) 1.0 0.8 1.5 1.0 1.2 1.5 1.5 1.6 1.2 1.2 1.5 1.2 1.2 0.9 1.4 1.7 1.6 1.4 1.1 1.0 1.8
Intensity Change (Log2) -0.12 -0.05 -0.3 -0.28 -0.16 -0.48 -0.48 -0.5 -0.34 -0.05 -0.02 -0.0 0.53 0.53 0.49 -0.14 -0.16 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 3.4 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0118 4.3028 3.5512 3.8304 3.5459 3.5445 1.3761 2.4141 2.0281 1.584 1.7577 1.8719 1.1641 1.9175 1.6587 1.539 1.2985 1.7012
Actin 0.0292 0.0002 0.0277 0.0857 0.0178 0.0002 0.021 0.0008 0.0061 0.0244 0.0009 0.002 0.0437 0.0015 0.0286 0.0015 0.0001 0.0043
Bud 0.0003 0.0022 0.0005 0.0006 0.0002 0.0001 0.001 0.0089 0.0051 0.0017 0.0007 0.0018 0.0057 0.023 0.0014 0.0015 0.0001 0.0018
Bud Neck 0.0016 0.0005 0.0014 0.0006 0.0004 0.0008 0.0005 0.0003 0.0005 0.0009 0.0006 0.0005 0.0018 0.0003 0.0015 0.0007 0.0002 0.0008
Bud Periphery 0.0003 0.0012 0.001 0.0007 0.0002 0 0.0017 0.0018 0.0034 0.0024 0.0004 0.0022 0.0054 0.0094 0.0011 0.0005 0.0001 0.0017
Bud Site 0.0032 0.0134 0.0052 0.0037 0.0005 0.0001 0.0026 0.0252 0.0088 0.0022 0.0017 0.0004 0.0079 0.0473 0.0142 0.0011 0.0001 0.0002
Cell Periphery 0.0009 0.0007 0.0007 0.0002 0.0002 0.0001 0.0007 0.0004 0.0004 0.0002 0.0001 0.0001 0.0006 0.0011 0.0006 0.0001 0 0.0002
Cytoplasm 0.642 0.7508 0.6964 0.6416 0.6403 0.8239 0.6933 0.8673 0.7984 0.7753 0.785 0.8903 0.653 0.6949 0.7365 0.6814 0.7535 0.8254
Cytoplasmic Foci 0.0249 0.0268 0.0178 0.0125 0.0248 0.0165 0.03 0.012 0.0251 0.0314 0.0704 0.0179 0.03 0.0264 0.0352 0.0533 0.0303 0.0374
Eisosomes 0.0004 0.0001 0.0003 0.0001 0.0002 0 0.0003 0.0001 0.0001 0.0003 0 0 0.0002 0.0001 0.0002 0 0 0
Endoplasmic Reticulum 0.0086 0.0031 0.0048 0.0016 0.0049 0.0031 0.0049 0.0028 0.0035 0.0022 0.0016 0.0031 0.009 0.004 0.0046 0.0062 0.0031 0.0047
Endosome 0.0293 0.0171 0.0224 0.0131 0.0334 0.0296 0.0335 0.0177 0.047 0.0661 0.0539 0.0408 0.0638 0.0184 0.0353 0.1025 0.1275 0.0325
Golgi 0.0079 0.0007 0.0062 0.0017 0.0044 0.0013 0.0105 0.0006 0.007 0.0232 0.0101 0.0041 0.0095 0.0049 0.009 0.0084 0.0018 0.0079
Lipid Particles 0.0126 0.0012 0.0055 0.0011 0.0091 0.0002 0.0271 0.0001 0.0061 0.0053 0.0054 0.0002 0.0068 0.0009 0.0111 0.0032 0.0001 0.0034
Mitochondria 0.0025 0.0003 0.0054 0.0019 0.0016 0.0004 0.0151 0.0005 0.0208 0.0243 0.0085 0.0026 0.0032 0.001 0.0075 0.0018 0.0006 0.0017
None 0.2044 0.1588 0.1827 0.2141 0.2266 0.1142 0.1286 0.0518 0.0417 0.0104 0.0223 0.0232 0.132 0.1542 0.0904 0.0816 0.0496 0.0668
Nuclear Periphery 0.0015 0.0005 0.001 0.0005 0.0048 0.0008 0.0012 0.0004 0.0018 0.0009 0.0007 0.0009 0.0039 0.0009 0.0016 0.0027 0.0008 0.0017
Nucleolus 0.0004 0.0005 0.0003 0.0003 0.0005 0 0.0003 0.0002 0.0001 0.0002 0.0005 0 0.0004 0.0002 0.0002 0.0004 0 0.0001
Nucleus 0.0009 0.0013 0.0009 0.0009 0.0014 0.0009 0.001 0.001 0.0014 0.0012 0.0009 0.0006 0.0015 0.0017 0.0008 0.0036 0.0007 0.001
Peroxisomes 0.0091 0.0024 0.0076 0.0045 0.0063 0.0001 0.0084 0.0002 0.0064 0.0045 0.0245 0.0002 0.0014 0.001 0.0084 0.0034 0.0001 0.0008
Punctate Nuclear 0.0041 0.0007 0.0011 0.0003 0.0182 0.0002 0.0015 0.0003 0.0018 0.0004 0.0013 0.0002 0.0012 0.0005 0.0012 0.0087 0.0002 0.0009
Vacuole 0.0149 0.0169 0.0098 0.0127 0.0034 0.0064 0.0159 0.0072 0.0117 0.019 0.0085 0.0069 0.0175 0.0077 0.0091 0.0335 0.0267 0.0055
Vacuole Periphery 0.0008 0.0007 0.0012 0.0018 0.0008 0.0011 0.0012 0.0006 0.0028 0.0034 0.0019 0.0021 0.0016 0.0007 0.0015 0.0037 0.0044 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.1846 14.9085 19.1355 21.8267 18.7008 18.9205 23.935 30.1092 22.8216 25.6888
Translational Efficiency 0.6119 0.8674 0.715 0.5419 0.6595 0.8203 0.6803 0.5641 0.6932 0.6168

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency