Standard name | |
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Human Ortholog | |
Description | Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 1 |
Bud | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 12 | 16 | 4 | 4 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 2 | 4 | 4 | 3 | 2 | 6 | 3 | 5 | 7 | 1 | 1 | 2 | 3 | 7 | 7 | 17 | 14 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 169 | 135 | 241 | 251 | 317 | 259 | 378 | 307 | 301 | 300 | 196 | 297 | 230 | 321 | 412 | 265 | 237 | 206 | 159 | 138 | 204 | 140 | 242 | 210 |
Endoplasmic Reticulum | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 12 | 12 | 8 | 2 | 0 | 2 | 9 | 11 | 9 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 2 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 11 | 0 | 0 | 2 | 16 | 43 | 16 | 79 | 96 | 94 | 49 | 2 | 2 | 1 | 6 | 1 | 3 | 2 | 1 | 5 | 6 | 9 | 7 |
Nucleus | 1 | 0 | 1 | 1 | 0 | 3 | 3 | 8 | 4 | 2 | 2 | 1 | 0 | 0 | 1 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 3 | 1 | 3 | 1 | 2 | 1 | 0 | 13 | 15 | 5 | 2 | 2 | 4 | 16 | 15 | 15 |
Unique Cell Count | 173 | 141 | 242 | 255 | 320 | 267 | 398 | 320 | 334 | 350 | 254 | 328 | 240 | 322 | 416 | 278 | 250 | 215 | 177 | 153 | 229 | 184 | 291 | 261 |
Labelled Cell Count | 179 | 149 | 247 | 260 | 327 | 283 | 436 | 339 | 399 | 422 | 318 | 358 | 245 | 328 | 428 | 308 | 287 | 239 | 177 | 153 | 229 | 184 | 291 | 261 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 4.4 | 6.1 | 5.6 | 5.9 | 4.9 | 5.0 | 5.5 | 4.4 | 4.4 | 4.3 | 4.8 | 5.9 | 6.0 | 6.1 | 8.8 | 8.8 | 8.6 | 5.5 | 5.5 | 6.0 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.5 | 1.0 | 1.2 | 1.5 | 1.5 | 1.6 | 1.2 | 1.2 | 1.5 | 1.2 | 1.2 | 0.9 | 1.4 | 1.7 | 1.6 | 1.4 | 1.1 | 1.0 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.05 | -0.3 | -0.28 | -0.16 | -0.48 | -0.48 | -0.5 | -0.34 | -0.05 | -0.02 | -0.0 | 0.53 | 0.53 | 0.49 | -0.14 | -0.16 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.4 | 3.9 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0118 | 4.3028 | 3.5512 | 3.8304 | 3.5459 | 3.5445 | 1.3761 | 2.4141 | 2.0281 | 1.584 | 1.7577 | 1.8719 | 1.1641 | 1.9175 | 1.6587 | 1.539 | 1.2985 | 1.7012 |
Actin | 0.0292 | 0.0002 | 0.0277 | 0.0857 | 0.0178 | 0.0002 | 0.021 | 0.0008 | 0.0061 | 0.0244 | 0.0009 | 0.002 | 0.0437 | 0.0015 | 0.0286 | 0.0015 | 0.0001 | 0.0043 |
Bud | 0.0003 | 0.0022 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.001 | 0.0089 | 0.0051 | 0.0017 | 0.0007 | 0.0018 | 0.0057 | 0.023 | 0.0014 | 0.0015 | 0.0001 | 0.0018 |
Bud Neck | 0.0016 | 0.0005 | 0.0014 | 0.0006 | 0.0004 | 0.0008 | 0.0005 | 0.0003 | 0.0005 | 0.0009 | 0.0006 | 0.0005 | 0.0018 | 0.0003 | 0.0015 | 0.0007 | 0.0002 | 0.0008 |
Bud Periphery | 0.0003 | 0.0012 | 0.001 | 0.0007 | 0.0002 | 0 | 0.0017 | 0.0018 | 0.0034 | 0.0024 | 0.0004 | 0.0022 | 0.0054 | 0.0094 | 0.0011 | 0.0005 | 0.0001 | 0.0017 |
Bud Site | 0.0032 | 0.0134 | 0.0052 | 0.0037 | 0.0005 | 0.0001 | 0.0026 | 0.0252 | 0.0088 | 0.0022 | 0.0017 | 0.0004 | 0.0079 | 0.0473 | 0.0142 | 0.0011 | 0.0001 | 0.0002 |
Cell Periphery | 0.0009 | 0.0007 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0011 | 0.0006 | 0.0001 | 0 | 0.0002 |
Cytoplasm | 0.642 | 0.7508 | 0.6964 | 0.6416 | 0.6403 | 0.8239 | 0.6933 | 0.8673 | 0.7984 | 0.7753 | 0.785 | 0.8903 | 0.653 | 0.6949 | 0.7365 | 0.6814 | 0.7535 | 0.8254 |
Cytoplasmic Foci | 0.0249 | 0.0268 | 0.0178 | 0.0125 | 0.0248 | 0.0165 | 0.03 | 0.012 | 0.0251 | 0.0314 | 0.0704 | 0.0179 | 0.03 | 0.0264 | 0.0352 | 0.0533 | 0.0303 | 0.0374 |
Eisosomes | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0086 | 0.0031 | 0.0048 | 0.0016 | 0.0049 | 0.0031 | 0.0049 | 0.0028 | 0.0035 | 0.0022 | 0.0016 | 0.0031 | 0.009 | 0.004 | 0.0046 | 0.0062 | 0.0031 | 0.0047 |
Endosome | 0.0293 | 0.0171 | 0.0224 | 0.0131 | 0.0334 | 0.0296 | 0.0335 | 0.0177 | 0.047 | 0.0661 | 0.0539 | 0.0408 | 0.0638 | 0.0184 | 0.0353 | 0.1025 | 0.1275 | 0.0325 |
Golgi | 0.0079 | 0.0007 | 0.0062 | 0.0017 | 0.0044 | 0.0013 | 0.0105 | 0.0006 | 0.007 | 0.0232 | 0.0101 | 0.0041 | 0.0095 | 0.0049 | 0.009 | 0.0084 | 0.0018 | 0.0079 |
Lipid Particles | 0.0126 | 0.0012 | 0.0055 | 0.0011 | 0.0091 | 0.0002 | 0.0271 | 0.0001 | 0.0061 | 0.0053 | 0.0054 | 0.0002 | 0.0068 | 0.0009 | 0.0111 | 0.0032 | 0.0001 | 0.0034 |
Mitochondria | 0.0025 | 0.0003 | 0.0054 | 0.0019 | 0.0016 | 0.0004 | 0.0151 | 0.0005 | 0.0208 | 0.0243 | 0.0085 | 0.0026 | 0.0032 | 0.001 | 0.0075 | 0.0018 | 0.0006 | 0.0017 |
None | 0.2044 | 0.1588 | 0.1827 | 0.2141 | 0.2266 | 0.1142 | 0.1286 | 0.0518 | 0.0417 | 0.0104 | 0.0223 | 0.0232 | 0.132 | 0.1542 | 0.0904 | 0.0816 | 0.0496 | 0.0668 |
Nuclear Periphery | 0.0015 | 0.0005 | 0.001 | 0.0005 | 0.0048 | 0.0008 | 0.0012 | 0.0004 | 0.0018 | 0.0009 | 0.0007 | 0.0009 | 0.0039 | 0.0009 | 0.0016 | 0.0027 | 0.0008 | 0.0017 |
Nucleolus | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0001 |
Nucleus | 0.0009 | 0.0013 | 0.0009 | 0.0009 | 0.0014 | 0.0009 | 0.001 | 0.001 | 0.0014 | 0.0012 | 0.0009 | 0.0006 | 0.0015 | 0.0017 | 0.0008 | 0.0036 | 0.0007 | 0.001 |
Peroxisomes | 0.0091 | 0.0024 | 0.0076 | 0.0045 | 0.0063 | 0.0001 | 0.0084 | 0.0002 | 0.0064 | 0.0045 | 0.0245 | 0.0002 | 0.0014 | 0.001 | 0.0084 | 0.0034 | 0.0001 | 0.0008 |
Punctate Nuclear | 0.0041 | 0.0007 | 0.0011 | 0.0003 | 0.0182 | 0.0002 | 0.0015 | 0.0003 | 0.0018 | 0.0004 | 0.0013 | 0.0002 | 0.0012 | 0.0005 | 0.0012 | 0.0087 | 0.0002 | 0.0009 |
Vacuole | 0.0149 | 0.0169 | 0.0098 | 0.0127 | 0.0034 | 0.0064 | 0.0159 | 0.0072 | 0.0117 | 0.019 | 0.0085 | 0.0069 | 0.0175 | 0.0077 | 0.0091 | 0.0335 | 0.0267 | 0.0055 |
Vacuole Periphery | 0.0008 | 0.0007 | 0.0012 | 0.0018 | 0.0008 | 0.0011 | 0.0012 | 0.0006 | 0.0028 | 0.0034 | 0.0019 | 0.0021 | 0.0016 | 0.0007 | 0.0015 | 0.0037 | 0.0044 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.1846 | 14.9085 | 19.1355 | 21.8267 | 18.7008 | 18.9205 | 23.935 | 30.1092 | 22.8216 | 25.6888 |
Translational Efficiency | 0.6119 | 0.8674 | 0.715 | 0.5419 | 0.6595 | 0.8203 | 0.6803 | 0.5641 | 0.6932 | 0.6168 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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