TheCellVision

TIF35

Standard name	TIF35 ORF YDR429C Aliases translation initiation factor eIF3 core subunit g
Description	eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation; eIF3 is also involved in programmed stop codon readthrough

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Scale images

No result found.

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Cytoplasm	0.13	0.07	0.2	0.14	0.17	0.19	0.27	0.42	0.25	0.34	0.26	0.28	0.14	0.19	0.3	–	0.12	0.13	0	0.08	0.06	0.09	0.08	0.07
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Endosome	0	0.05	0	0	0.07	0.07	0.05	0.05	0	0	0	0	0	0	0.05	–	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Mitochondria	0.1	0.57	0	0.07	0	0.29	0.34	0.17	0.62	0.49	0.61	0.62	0	0	0	–	0.09	0.09	0.06	0	0	0	0	0
Nucleus	0.67	0.51	0.66	0.72	0.61	0.44	0.37	0.28	0.25	0.19	0.21	0.16	0.82	0.75	0.58	–	0.81	0.75	0.58	0.57	0.53	0.48	0.48	0.36
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0.05	0.05	0.07
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0.06	0	0.06	0.07	0	0	0	0.05	0	0	0	–	0	0	0.08	0.06	0.06	0.09	0.07	0.17
Vac/Vac Membrane	0.18	0.1	0.06	0.07	0.13	0.1	0.09	0.14	0.06	0.09	0.11	0.11	0	0	0.08	–	0	0.07	0.15	0.15	0.18	0.19	0.19	0.18

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	1	1
Bud	0	0	0	2	1	1	7	2	5	6	2	13	0	0	0	–	0	0	0	0	7	3	12	15
Bud Neck	0	0	3	2	5	5	3	7	2	7	0	5	1	2	2	–	0	0	0	0	1	0	3	6
Bud Site	0	0	0	0	0	0	1	1	6	3	2	13	0	0	0	–	0	0	–	–	–	–	–	–
Cell Periphery	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Cytoplasm	13	6	64	48	69	86	137	192	80	142	90	142	43	68	117	–	10	15	5	7	28	19	42	42
Endoplasmic Reticulum	1	0	3	0	1	0	0	0	0	0	0	0	0	1	0	–	3	5	0	0	2	1	3	1
Endosome	1	4	13	15	29	33	26	21	2	10	5	10	2	7	18	–	2	3	3	3	11	1	7	21
Golgi	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	–	0	0	1	0	0	0	1	0
Mitochondria	10	46	8	22	17	132	175	78	198	206	211	310	5	3	3	–	8	10	8	4	19	5	17	21
Nucleus	68	41	208	244	245	204	187	130	79	82	73	79	252	267	228	–	70	85	81	54	242	106	249	215
Nuclear Periphery	0	2	1	0	1	0	1	0	2	1	0	2	0	0	0	–	1	0	0	0	1	0	2	4
Nucleolus	0	3	1	0	0	2	0	1	1	1	0	1	0	0	1	–	2	1	4	2	11	11	23	38
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	–	0	0	1	0	0	0	0	1
SpindlePole	0	1	12	13	25	18	29	32	8	18	10	27	7	1	1	–	0	0	10	5	28	18	33	99
Vac/Vac Membrane	18	8	18	22	52	46	48	65	20	37	38	53	13	14	32	–	3	8	20	14	83	42	100	105
Unique Cell Count	101	80	315	337	401	460	509	461	321	421	348	499	307	355	395		86	114	141	96	456	220	517	597
Labelled Cell Count	112	111	332	368	445	527	614	530	403	513	431	655	323	363	406		99	127	141	96	456	220	517	597

Yeast GFP Assignment

Cytoplasm

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	4.5	4.0	5.6	5.4	5.7	4.6	4.5	5.1	4.0	4.1	3.9	3.8	5.2	5.5	5.2	–	6.2	6.8	6.1	6.4	6.1
Std Deviation (1e^-4)	0.5	0.9	1.6	1.6	1.7	1.7	1.4	1.9	1.6	1.6	1.3	0.8	0.9	1.2	1.2	–	1.9	1.8	2.1	2.3	2.2
Intensity Change (Log₂)	–	–	–	-0.05	0.03	-0.27	-0.31	-0.14	-0.49	-0.43	-0.52	-0.56	-0.09	-0.02	-0.1	–	0.16	0.28	0.14	0.2	0.13

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Actin	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Bud Neck	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Bud Site	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Cell Periphery	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Cyto	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endoplasmic Reticulum	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Endosome	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Golgi	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Mitochondria	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Nuclear Periphery	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Nuc	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleolus	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Peroxisomes	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
SpindlePole	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Vac	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cortical Patches	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Cytoplasm	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Nucleus	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0
Vacuole	–	–	–	–	–	–	–	–	–	–	–	–	–	–	0.5

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	-0.7082	-0.1135	-0.3553	-0.5792	-0.7389	-0.2303	1.3442	1.1913	1.2323	1.3877	1.1235	1.1035	-0.9299	-0.2895	-0.5306	-0.4704	-0.9806	-0.5904
Actin	0.0009	0.0005	0.0008	0.0005	0.0005	0.0002	0.0232	0.0002	0.0202	0.0002	0.016	0.0005	0.012	0.0002	0.0004	0.0144	0.0005	0.0004
Bud	0.0002	0.0037	0.0001	0	0	0	0.0015	0	0.0002	0	0.0003	0.0001	0.0002	0.0003	0.0001	0.0002	0.0001	0.0001
Bud Neck	0.0049	0.0007	0.0005	0.0008	0.0008	0.0036	0.0161	0.0005	0.0013	0.0005	0.0016	0.0037	0.0016	0.0006	0.0005	0.0015	0.0011	0.0032
Bud Periphery	0.0002	0.0007	0.0001	0	0.0001	0.0001	0.0023	0.0001	0.0002	0	0.0007	0.0001	0.0002	0.0002	0	0.0002	0	0.0001
Bud Site	0.0019	0.0021	0.0004	0.0003	0.0001	0.0001	0.0159	0.0004	0.0026	0.0002	0.0013	0.0002	0.0008	0.0018	0.0005	0.0011	0.0003	0.0001
Cell Periphery	0.0002	0.0002	0	0	0.0001	0	0.0008	0.0001	0.0001	0	0.0002	0	0.0001	0.0001	0	0.0001	0	0
Cytoplasm	0.0576	0.0831	0.0529	0.0447	0.0417	0.0316	0.0496	0.0446	0.0486	0.0513	0.0474	0.0309	0.1206	0.1068	0.1099	0.0481	0.2375	0.0396
Cytoplasmic Foci	0.0076	0.0057	0.0063	0.0022	0.002	0.0013	0.0297	0.001	0.0089	0.001	0.0097	0.0041	0.0132	0.0052	0.0046	0.0055	0.0301	0.0119
Eisosomes	0.0002	0.0002	0.0001	0.0002	0.0002	0	0.0006	0.0001	0.0011	0.0001	0.0004	0.0001	0.0002	0	0	0.0002	0	0
Endoplasmic Reticulum	0.0021	0.0039	0.0018	0.0012	0.0028	0.0006	0.005	0.0021	0.0016	0.0005	0.0036	0.0011	0.0073	0.0031	0.0038	0.0007	0.0059	0.0007
Endosome	0.0061	0.0035	0.0026	0.0016	0.0047	0.0007	0.0354	0.0013	0.0025	0.0005	0.0191	0.0031	0.0143	0.003	0.0034	0.0047	0.0054	0.0018
Golgi	0.0006	0.0001	0.0001	0.0001	0.0001	0	0.0062	0	0.0017	0	0.0081	0.0004	0.0018	0.0002	0.0001	0.004	0.0001	0
Lipid Particles	0.0034	0.0012	0.0006	0.0003	0.0009	0.0001	0.0227	0.0003	0.0041	0.0001	0.0351	0.002	0.0042	0.0004	0.0005	0.0064	0.0014	0.0002
Mitochondria	0.0008	0.0012	0.001	0.0007	0.0017	0.0002	0.0032	0.0009	0.0015	0.0005	0.0111	0.0057	0.0009	0.0005	0.0005	0.0017	0.0006	0.0003
None	0.2117	0.3276	0.1913	0.2964	0.2808	0.1199	0.2205	0.2531	0.2461	0.3066	0.1626	0.1158	0.1242	0.0692	0.1046	0.0558	0.0815	0.0682
Nuclear Periphery	0.0132	0.0173	0.0154	0.008	0.0249	0.0042	0.0188	0.0095	0.0176	0.0049	0.0362	0.0069	0.0247	0.0158	0.0288	0.0134	0.0192	0.0115
Nucleolus	0.0179	0.0085	0.0073	0.014	0.0091	0.0155	0.0172	0.0077	0.0078	0.0059	0.0089	0.0147	0.0056	0.0044	0.0042	0.0042	0.0092	0.0086
Nucleus	0.5974	0.5111	0.6645	0.5879	0.5785	0.7592	0.413	0.6525	0.5692	0.5837	0.5346	0.7375	0.6166	0.7489	0.6853	0.8176	0.5502	0.8098
Peroxisomes	0.0019	0.0003	0.0001	0.0002	0.0002	0	0.0184	0.0001	0.0174	0.0001	0.0387	0.0014	0.0057	0.0002	0.0001	0.0057	0.0005	0.0001
Punctate Nuclear	0.0673	0.0245	0.0527	0.0401	0.0474	0.0619	0.0899	0.0234	0.0463	0.0436	0.0601	0.0707	0.0417	0.0375	0.0509	0.0138	0.0547	0.0423
Vacuole	0.0034	0.0032	0.0011	0.0005	0.0028	0.0005	0.0086	0.0015	0.0007	0.0003	0.0025	0.001	0.0034	0.0015	0.0015	0.0004	0.0014	0.001
Vacuole Periphery	0.0004	0.0005	0.0003	0.0001	0.0009	0.0001	0.0013	0.0006	0.0003	0.0001	0.0018	0.0002	0.0006	0.0003	0.0004	0.0002	0.0003	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	215.9998	203.2533	182.7616	175.3119	189.0142	189.8142	170.7539	158.2867	163.971	170.0145
Translational Efficiency	2.5488	2.4239	2.6891	2.4271	2.5733	2.4408	2.1798	2.7029	2.1194	2.3001

Hit Data

Dataset	Hit
Protein Abundance	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘