Standard name
Description Protein of unknown function; involved in negative regulation of expression of spliceosome components PRP4 and PRP3; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.07 0.08 0.09 0.09 0.1 0.09 0.07 0.07 0.07 0.07 0.11 0.21 0.29 0.17 0.17 0.13 0 0 0 0 0.05 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.06 0.05 0.09 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.4 0.77 0.76 0.56 0.71 0.8 0.76 0.89 0.87 0.87 0.89 0.11 0.12 0.39 0.09 0.09 0.13 0 0.05 0.05 0 0 0.05
Nucleus 0.8 0.7 0.55 0.57 0.53 0.45 0.37 0.38 0.39 0.34 0.37 0.38 0.79 0.62 0.23 0.63 0.62 0.67 0.52 0.61 0.49 0.7 0.42 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.09 0.07 0.12 0.12 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.06 0.1 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0.07 0 0.08 0.2
Vac/Vac Membrane 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0.11 0.13 0.11 0.1 0.12 0.24 0.07 0.18 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud 1 0 4 0 1 3 4 10 4 7 1 6 0 1 11 0 0 1 0 2 0 1 6 4
Bud Neck 1 0 1 0 0 0 5 2 0 2 0 0 0 0 0 0 0 1 0 2 0 0 3 3
Bud Site 1 1 1 0 4 9 14 24 7 6 7 10 0 1 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 15 8 14 21 23 41 43 19 23 12 19 21 45 72 37 28 29 1 3 2 2 14 15
Endoplasmic Reticulum 0 0 0 1 0 0 0 1 0 1 0 0 0 1 1 13 9 8 0 0 0 0 3 2
Endosome 11 8 0 0 5 3 1 2 0 0 0 0 1 10 22 14 8 19 13 5 0 0 9 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 3 0 0 0 1 1
Mitochondria 52 102 85 127 139 175 323 382 231 300 143 232 22 25 97 20 14 29 3 13 6 1 10 14
Nucleus 199 177 61 95 130 110 149 192 102 118 60 98 153 131 57 139 99 144 148 180 59 121 119 70
Nuclear Periphery 2 0 0 0 0 0 1 2 1 0 3 2 0 0 0 2 0 1 3 3 0 0 0 0
Nucleolus 34 6 1 2 4 7 11 7 4 7 3 6 2 3 2 1 2 0 56 27 7 20 32 37
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 5 2 3 1 9 9 23 49 4 11 7 3 6 7 1 9 8 9 16 14 8 7 22 55
Vac/Vac Membrane 7 17 0 0 4 6 2 8 1 1 1 2 8 10 30 24 21 24 28 35 29 12 50 49
Unique Cell Count 250 252 110 168 247 247 405 501 260 345 164 261 194 213 249 222 160 216 286 293 121 173 283 279
Labelled Cell Count 319 328 164 240 317 345 574 722 373 477 237 378 213 234 294 266 189 265 286 293 121 173 283 279


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.0 3.2 3.4 3.6 3.2 2.9 3.2 2.7 2.8 3.0 2.8 4.8 4.5 4.0 6.1 6.1 6.5 4.6 4.5 4.9
Std Deviation (1e-4) 0.9 0.9 0.7 1.0 1.3 1.0 1.0 1.3 0.8 1.0 1.1 1.3 1.1 1.3 1.5 1.6 1.8 1.7 0.8 1.2 1.5
Intensity Change (Log2) 0.1 0.17 0.03 -0.11 0.03 -0.22 -0.2 -0.1 -0.2 0.59 0.5 0.33 0.94 0.96 1.05 0.55 0.51 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.6 4.0 3.6