Standard name
Human Ortholog
Description Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.99 0.98 0.94 0.88 0.86 0.85 0.83 0.76 0.83 0.98 0.99 0.98 0.95 0.94 0.97 0.94 0.95 0.95 0.93 0.9 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0.09 0.15 0.19 0.26 0.25 0.36 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.07 0 0 0.05 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 1
Bud 1 0 0 1 1 3 10 4 9 4 9 0 1 0 0 0 1 0 0 0 0 6 3
Bud Neck 0 0 1 1 0 0 1 2 3 0 0 0 0 1 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 4 1 0 5 3 2 1 0 0 1 1 5 6 7 0 0 0 0 0 0
Cytoplasm 230 23 336 535 439 574 560 331 461 274 328 125 393 406 180 203 207 229 33 66 87 305 312
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 0 0 0 1 2 7 11 4 0 0 0 0 1 2
Endosome 1 0 0 0 0 2 1 0 0 2 0 0 1 2 0 1 0 2 0 0 0 4 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 2
Mitochondria 12 1 1 4 44 96 122 100 138 129 99 0 1 0 1 2 3 2 0 1 3 5 4
Nucleus 1 0 2 1 5 10 7 5 6 1 11 2 0 3 3 1 6 0 0 0 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 2 2 0 0 0 0 0 1 0 0 0 1 0 0 0 5 4
Vac/Vac Membrane 0 0 2 10 24 43 28 12 30 11 8 0 0 4 5 23 8 4 0 0 0 1 0
Unique Cell Count 236 24 338 545 466 653 654 391 556 362 395 127 396 415 190 217 214 244 35 70 94 338 348
Labelled Cell Count 246 24 345 557 514 729 737 460 650 423 458 127 400 422 203 248 236 244 35 70 94 338 348


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.7 5.4 5.9 5.1 4.6 4.7 4.4 4.3 4.0 4.2 5.5 6.4 6.0 8.5 9.0 8.8 5.0 5.9 6.0
Std Deviation (1e-4) 0.6 0.4 1.5 1.5 1.5 1.4 1.3 1.7 1.1 1.3 1.0 0.8 1.3 1.1 1.6 2.0 1.9 0.9 1.4 1.3
Intensity Change (Log2) 0.21 0.34 0.13 -0.01 0.02 -0.07 -0.12 -0.21 -0.17 0.24 0.45 0.35 0.87 0.95 0.92 0.09 0.34 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0.8 -0.3 -1.2 -1.4 -1.5 -1.7 -2.3 -1.7 0.8 1.7 0.6 -0.2 -0.4 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4284 2.3319 1.9551 1.6901 1.0235 1.9133 1.0199 2.287 1.5382 2.2706 1.1585 1.9287 1.5031 2.1426 1.5249 1.2916 1.0995 1.3703
Actin 0.0106 0.0011 0.0079 0.001 0.0007 0.007 0.0461 0.0037 0.0113 0.0349 0.0344 0.0004 0.0515 0.0001 0.0175 0.0138 0.0438 0.0271
Bud 0.0016 0.0138 0.0054 0.0012 0.0117 0.0045 0.0029 0.0004 0.0001 0.0021 0.0009 0.0056 0.0004 0.0001 0.0001 0.0005 0.0003 0.0002
Bud Neck 0.0046 0.0008 0.0019 0.0004 0.0009 0.0048 0.0036 0.002 0.0003 0.0297 0.0066 0.0006 0.0078 0.0001 0.0002 0.0005 0.0019 0.0011
Bud Periphery 0.0009 0.0018 0.0014 0.0005 0.0032 0.002 0.0024 0.0003 0.0001 0.0033 0.0016 0.0021 0.0005 0 0.0001 0.0005 0.0003 0.0001
Bud Site 0.0049 0.0194 0.0049 0.0003 0.0008 0.0029 0.0051 0.0127 0.0004 0.0132 0.0089 0.0002 0.0019 0.0002 0.0002 0.0011 0.0066 0.0001
Cell Periphery 0.0002 0.0004 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0008 0.0003 0 0.0002 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.6262 0.728 0.6944 0.7323 0.6452 0.7036 0.636 0.7991 0.7641 0.6251 0.646 0.838 0.7225 0.9022 0.8654 0.8421 0.8366 0.8548
Cytoplasmic Foci 0.023 0.0104 0.0137 0.0112 0.0231 0.0219 0.0353 0.003 0.0092 0.0133 0.0468 0.0057 0.0134 0.002 0.0041 0.0174 0.018 0.0129
Eisosomes 0.0001 0 0.0001 0 0 0 0.0002 0 0.0001 0.0006 0.0007 0 0.0003 0 0.0002 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0032 0.0025 0.0029 0.0032 0.0013 0.002 0.0022 0.0011 0.0024 0.0018 0.0065 0.0014 0.0049 0.0011 0.0029 0.0026 0.0021 0.0016
Endosome 0.0084 0.0041 0.0044 0.0053 0.0034 0.0061 0.0111 0.0005 0.0051 0.0183 0.0255 0.002 0.0109 0.0005 0.0036 0.0088 0.0214 0.0035
Golgi 0.0014 0.0002 0.0004 0.0002 0.0002 0.001 0.0051 0.0002 0.0012 0.0096 0.0144 0.0001 0.0049 0 0.0016 0.0093 0.003 0.0052
Lipid Particles 0.0015 0.0001 0.0004 0.0001 0.0002 0.0008 0.0122 0.0001 0.0042 0.009 0.0247 0 0.0098 0 0.002 0.0112 0.0086 0.0012
Mitochondria 0.0019 0.0012 0.001 0.0006 0.0005 0.0013 0.0018 0.0001 0.0019 0.0207 0.023 0.0004 0.0028 0.0001 0.0005 0.0031 0.0005 0.0196
None 0.2846 0.1883 0.2107 0.2074 0.2802 0.2068 0.2123 0.174 0.1899 0.171 0.0735 0.1386 0.087 0.0907 0.0841 0.0737 0.0438 0.0633
Nuclear Periphery 0.0041 0.0028 0.0037 0.0055 0.0011 0.0024 0.0008 0.0003 0.0014 0.0017 0.0119 0.0005 0.0163 0.0004 0.002 0.0012 0.0011 0.0008
Nucleolus 0.0009 0.0009 0.0012 0.0003 0.0005 0.0005 0.0002 0.0001 0.0002 0.0006 0.0019 0 0.0018 0 0 0.0001 0.001 0
Nucleus 0.0073 0.0167 0.0258 0.0147 0.0064 0.0136 0.0023 0.0012 0.0021 0.0034 0.0035 0.0031 0.0299 0.0018 0.0034 0.0018 0.0016 0.0051
Peroxisomes 0.0056 0.0003 0.0009 0.0005 0.0009 0.0012 0.0147 0.0001 0.0037 0.0355 0.0346 0.0001 0.021 0 0.0103 0.0093 0.0024 0.0012
Punctate Nuclear 0.0074 0.0057 0.0171 0.013 0.0184 0.0158 0.0037 0.0004 0.0015 0.0024 0.0314 0.0006 0.0097 0.0002 0.0007 0.0022 0.0035 0.0013
Vacuole 0.0012 0.0015 0.0015 0.0018 0.0011 0.0012 0.0015 0.0003 0.0006 0.002 0.0016 0.0004 0.0018 0.0004 0.0007 0.0005 0.0027 0.0005
Vacuole Periphery 0.0004 0.0002 0.0003 0.0005 0.0002 0.0003 0.0002 0 0.0002 0.0011 0.0014 0.0001 0.0008 0 0.0002 0.0003 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.7439 8.0685 12.7228 16.0151 14.6279 22.552 15.2964 19.2715 18.5024 15.7753
Translational Efficiency 1.2424 2.3761 1.3595 1.2036 1.1613 1.443 1.5338 1.3168 1.2003 1.2387

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Apa2

Apa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available