Standard name
Description Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.12 0.15 0.13 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.13 0 0.05 0.05 0.18 0.29 0.16 0.31 0.37 0.45 0.41 0 0 0 0 0 0.06 0 0 0 0 0 0
Nucleus 0.94 0.95 0.95 0.92 0.93 0.87 0.84 0.89 0.81 0.83 0.83 0.79 0.94 0.87 0.83 0.8 0.83 0.81 0.89 0.94 0.9 0.88 0.84 0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.08 0 0 0 0 0.05 0.06 0.07 0.08 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0.05 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 1 4 7 5 6 11 11 0 0 0 0 1 0 1 0 1 0 3 5
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 1 0 3 4 17 17 8 17 26 27 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm 4 0 7 16 14 17 22 21 14 6 9 8 23 73 115 31 19 12 0 0 0 4 5 4
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 1 0 0 0 1 0 2 3 2 4 0 0 0 0 1 1
Endosome 0 0 0 0 0 1 1 2 0 0 0 0 0 1 0 0 3 3 1 0 0 1 2 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 2 0 0 0 2 1
Mitochondria 28 8 8 18 22 76 216 122 154 290 226 281 4 1 0 8 1 9 0 0 1 1 3 9
Nucleus 169 57 264 305 443 361 617 665 400 657 411 538 446 542 633 193 167 128 164 73 250 189 361 364
Nuclear Periphery 0 1 2 11 3 4 21 29 8 3 8 3 7 1 4 0 0 2 0 0 0 0 0 1
Nucleolus 5 5 4 2 8 13 37 44 37 62 44 60 1 0 1 6 1 4 1 0 4 1 5 9
Peroxisomes 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 4 1 1 0 1 2 0 8 0 0 0 2 0 2 0 3 5
Vac/Vac Membrane 2 3 3 5 7 9 12 9 5 11 4 12 2 8 9 14 11 13 7 3 12 10 35 47
Unique Cell Count 180 60 277 330 477 417 734 751 496 791 498 681 476 622 763 242 202 158 185 78 278 216 429 458
Labelled Cell Count 208 75 292 357 501 487 950 920 633 1054 739 941 486 628 773 257 208 175 185 78 278 216 429 458


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.0 6.1 6.1 5.7 4.9 4.7 4.7 4.6 4.4 4.3 4.3 7.3 7.0 7.1 9.1 9.5 8.3 7.2 7.2 7.5
Std Deviation (1e-4) 0.8 1.0 1.3 1.7 1.5 1.6 1.5 1.0 1.4 1.0 1.3 1.0 1.2 1.4 1.5 2.5 2.3 2.3 1.7 1.3 1.4
Intensity Change (Log2) – – – 0.01 -0.08 -0.31 -0.36 -0.35 -0.39 -0.45 -0.5 -0.49 0.28 0.21 0.22 0.6 0.66 0.46 0.25 0.25 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 0.3 1.1 0.4 0.2 0.2 0 -0.7 0 1.6 4.5 5.6 4.5 3.3 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.6 1.2 0 0 0 0 0 0 0 0 0 0 0.3 0 1.5
Nucleus -1.5 -1.3 -3.8 -4.8 -3.3 -5.6 -5.1 -5.1 -6.2 -0.9 -3.7 -5.1 -5.5 -4.5 -4.8
Nuclear Periphery 2.2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 2.8 3.8