Standard name
Description Non-essential subunit of the NuA4 acetyltransferase complex; Esa1p-associated factor; relocalizes to the cytosol in response to hypoxia

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.07 0.25 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.18 0 0.05 0 0.10 0.10 0.12 0.46 0.29 0.38 0.41 0 0 0 0.09 0.06 0.05 0 0 0 0 0 0
Nucleus 0.89 0.89 0.91 0.90 0.92 0.88 0.85 0.84 0.71 0.73 0.73 0.69 0.92 0.91 0.89 0.70 0.67 0.72 0.72 0.80 0.71 0.71 0.72 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.15 0 0.05 0 0.06 0.08 0.05 0.12 0.07 0.06 0.10 0.06 0.05 0 0.08 0.07 0.05 0.15 0.09 0.13 0.08 0.10 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.07 0.07 0.06 0.06 0.10 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 4
Bud 0 0 0 0 0 1 4 1 3 5 2 5 0 1 0 0 0 0 4 0 3 0 2 1
Bud Neck 0 0 0 0 0 1 0 4 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 1 2 2 4 2 18 17 11 27 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 1 7 9 14 4 23 24 12 19 6 14 6 19 28 6 20 10 0 0 0 1 5 2
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 3 2 0 0 0 0 1 2
Endosome 1 0 1 5 1 4 10 5 1 5 4 2 1 2 1 2 1 0 4 0 6 1 6 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 2 2 0 0 1
Mitochondria 11 23 2 14 1 35 71 74 266 173 156 270 0 7 4 8 5 3 1 0 2 3 5 3
Nucleus 242 116 188 244 320 307 581 521 416 428 300 459 182 323 333 62 54 43 214 110 165 94 253 211
Nuclear Periphery 2 0 1 1 1 2 9 3 5 13 5 4 1 0 3 3 1 0 0 0 1 0 1 2
Nucleolus 34 19 9 14 13 20 57 30 70 43 24 64 11 17 16 7 6 3 44 12 30 11 36 53
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 4 7
SpindlePole 5 0 11 12 9 0 9 21 3 2 7 6 5 11 8 0 0 1 6 0 4 4 6 13
Vac/Vac Membrane 3 2 1 3 1 0 3 7 13 12 9 14 3 3 1 8 4 4 19 8 14 13 22 11
Unique Cell Count 271 131 207 272 346 347 683 618 583 588 411 664 198 355 373 88 81 60 299 138 233 133 354 332
Labelled Cell Count 306 162 221 303 362 376 772 692 809 717 526 865 209 385 394 98 95 67 299 138 233 133 354 332

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.7 7.5 6.6 7.2 5.9 6.0 6.1 5.0 5.3 5.3 5.2 6.7 7.0 6.7 7.4 8.4 9.0 6.6 7.3 7.5
Std Deviation (1e-4) 1.0 1.2 1.5 1.4 1.5 1.2 1.4 1.5 1.4 1.1 1.2 1.2 1.2 1.4 1.5 1.8 2.1 2.5 1.6 1.3 1.4
Intensity Change (Log2) -0.18 -0.05 -0.34 -0.32 -0.29 -0.59 -0.49 -0.51 -0.51 -0.15 -0.1 -0.16 -0.02 0.17 0.27 -0.18 -0.04 0.01

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2.9 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.5 0 4.2 4.3 4.7 11.7 8.5 9.9 10.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 -0.3 0.7 1.9 0.3 3.2 1.5 0.8 2.4 0.6 0.2 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.5 -1.7 0 0 -1.2 0 0 -2.5 0 -1.4 -1.3 -2.1 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0