ORF
Description Putative protein of unknown function; conserved among S. cerevisiae strains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.96 0.99 0.96 0.91 0.75 0.71 0.7 0.7 0.69 1.0 0.98 0.99 0.96 1.0 0.97 0.97 0.94 0.93 0.91 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.05 0 0.21 0.28 0.41 0.37 0.48 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.07 0 0.05 0 0 0 0 0.09 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 1 1 4 0 0 2 4 7 6 12 15 1 2 1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0
Cell Periphery 1 1 0 0 4 0 3 3 3 7 11 3 2 6 2 0 1 0 0 0 0 0 0
Cytoplasm 431 211 115 304 472 340 511 346 490 375 498 204 494 499 22 19 68 420 196 119 69 222 276
Endoplasmic Reticulum 1 1 0 0 2 0 1 0 7 0 1 1 1 2 0 2 1 0 0 0 0 1 1
Endosome 0 0 0 0 1 1 0 0 2 0 0 0 1 0 0 0 0 2 1 1 0 3 4
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 1 1
Mitochondria 2 1 6 14 14 80 192 197 258 257 355 1 1 0 0 0 1 2 0 1 0 2 2
Nucleus 4 2 1 1 2 3 15 9 12 8 8 1 3 1 0 0 0 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 4 3 5 1 6 0 1 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 1 1 0 0 1 0 2 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Vac/Vac Membrane 1 1 0 0 16 5 44 35 31 25 16 0 3 0 2 0 1 1 5 2 2 3 2
Unique Cell Count 434 214 120 306 490 375 678 484 701 539 724 205 503 502 23 19 70 435 209 129 77 239 295
Labelled Cell Count 441 219 126 319 513 432 776 601 816 686 912 211 512 509 26 21 74 435 209 129 77 239 295


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 9.2 4.9 6.2 6.6 5.0 4.6 4.1 4.3 4.0 3.9 8.8 9.1 8.7 9.7 9.2 9.3 7.4 9.1 9.5
Std Deviation (1e-4) 1.1 1.2 0.8 1.0 1.6 1.0 0.9 0.9 0.9 0.7 0.8 1.2 1.1 1.0 1.3 1.0 1.7 1.0 1.4 1.4
Intensity Change (Log2) 0.36 0.44 0.05 -0.07 -0.25 -0.19 -0.29 -0.32 0.85 0.91 0.84 0.99 0.92 0.94 0.61 0.9 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.6 0.3 -1.8 -5.0 -5.6 -6.0 -6.0 -6.2 2.4 1.6 3.1 0 0.9 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 0 4.1 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.9 3.0 0 0 0 0 0 0 0 0 0