Standard name
Description GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0.06 0.09 0.11 0.08 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.11 0.31 0.11 0.33 0.22 0.19 0.24 0.24 0.27 0.21 0.26 0.24 0.17 0.06 0.08 0.10 0 0 0 0 0.07 0.05
Nucleus 0.79 0.83 0.40 0.16 0.16 0.28 0.40 0.46 0.31 0.45 0.32 0.52 0.12 0.05 0 0.43 0.42 0.51 0.51 0.67 0.26 0.22 0.15 0.15
Nuclear Periphery 0 0 0 0 0 0.07 0.06 0.06 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.32 0.17 0.60 0.84 0.74 0.62 0.58 0.47 0.61 0.52 0.62 0.45 0.84 0.86 0.83 0.45 0.46 0.44 0.39 0.26 0.64 0.57 0.49 0.39
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.05 0.05 0.06 0.13 0.22 0.30
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 2 2 1 1 0 0 0 0 1 1 0 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 3 5 3 1 2 9 0 1 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 3 2 4 4 6 4 6 5 3 7 1 2 18 35 47 38 39 17 1 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 5 2 0 0 0 0 0 0
Endosome 0 2 0 0 3 1 2 1 1 0 2 0 3 1 6 3 1 0 2 0 0 2 2 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2
Mitochondria 20 6 15 23 13 25 42 28 22 38 40 50 78 96 69 32 34 26 0 0 1 6 18 14
Nucleus 204 251 57 12 20 21 75 70 29 72 46 124 37 20 17 214 171 131 146 226 40 48 41 39
Nuclear Periphery 1 1 1 0 2 5 11 9 3 5 9 9 0 1 0 3 0 0 3 3 2 1 4 6
Nucleolus 82 53 84 63 91 47 110 71 57 83 91 106 254 336 345 221 191 114 111 88 97 125 134 104
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 1 8 11 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 1 2 2 0 3 2 2 1 1 1 1 9 20 1 1 0 1 0 0 2 4 4
Vac/Vac Membrane 2 8 4 0 4 0 1 2 1 0 1 2 1 4 4 33 29 11 15 18 8 28 61 81
Unique Cell Count 257 304 141 75 123 76 189 151 93 160 146 237 304 392 418 494 412 257 287 340 153 220 277 271
Labelled Cell Count 312 323 166 104 142 103 253 194 122 207 196 305 394 513 522 554 472 303 287 340 153 220 277 271

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleolus, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.2 29.7 22.5 18.1 16.0 12.0 9.9 9.7 9.5 8.1 8.6 8.7 26.6 23.3 22.4 29.6 36.9 31.7 22.1 19.8 20.7
Std Deviation (1e-4) 8.3 8.2 6.6 6.3 5.9 5.0 3.2 3.1 2.7 2.4 2.5 2.5 10.7 9.7 10.2 13.7 16.8 14.3 6.5 5.7 6.1
Intensity Change (Log2) -0.31 -0.49 -0.9 -1.18 -1.22 -1.24 -1.47 -1.39 -1.38 0.24 0.05 -0.0 0.4 0.72 0.5 -0.02 -0.18 -0.12

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.7 0 4.0 2.8 1.9 2.7 3.0 3.6 2.6 3.6 3.5 1.7 -1.7 -0.9 -0.2
Nucleus -3.5 -4.1 -2.3 -1.2 -0.3 -1.9 -1.9 -2.9 0.9 -6.5 -10.1 -11.1 -0.2 -0.7 1.2
Nuclear Periphery 0 0 0 2.5 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.7 2.5 0.3 -0.3 -2.1 0.3 -1.3 0.5 -2.8 5.5 6.5 5.6 -3.1 -2.7 -2.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0