Standard name | |
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Human Ortholog | |
Description | GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 3 | 1 | 2 | 9 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 2 | 4 | 4 | 6 | 4 | 6 | 5 | 3 | 7 | 1 | 2 | 18 | 35 | 47 | 38 | 39 | 17 | 1 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2 | 0 | 0 | 3 | 1 | 2 | 1 | 1 | 0 | 2 | 0 | 3 | 1 | 6 | 3 | 1 | 0 | 2 | 0 | 0 | 2 | 2 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 20 | 6 | 15 | 23 | 13 | 25 | 42 | 28 | 22 | 38 | 40 | 50 | 78 | 96 | 69 | 32 | 34 | 26 | 0 | 0 | 1 | 6 | 18 | 14 |
Nucleus | 204 | 251 | 57 | 12 | 20 | 21 | 75 | 70 | 29 | 72 | 46 | 124 | 37 | 20 | 17 | 214 | 171 | 131 | 146 | 226 | 40 | 48 | 41 | 39 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 2 | 5 | 11 | 9 | 3 | 5 | 9 | 9 | 0 | 1 | 0 | 3 | 0 | 0 | 3 | 3 | 2 | 1 | 4 | 6 |
Nucleolus | 82 | 53 | 84 | 63 | 91 | 47 | 110 | 71 | 57 | 83 | 91 | 106 | 254 | 336 | 345 | 221 | 191 | 114 | 111 | 88 | 97 | 125 | 134 | 104 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 8 | 11 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 2 | 2 | 0 | 3 | 2 | 2 | 1 | 1 | 1 | 1 | 9 | 20 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 4 | 4 |
Vac/Vac Membrane | 2 | 8 | 4 | 0 | 4 | 0 | 1 | 2 | 1 | 0 | 1 | 2 | 1 | 4 | 4 | 33 | 29 | 11 | 15 | 18 | 8 | 28 | 61 | 81 |
Unique Cell Count | 257 | 304 | 141 | 75 | 123 | 76 | 189 | 151 | 93 | 160 | 146 | 237 | 304 | 392 | 418 | 494 | 412 | 257 | 287 | 340 | 153 | 220 | 277 | 271 |
Labelled Cell Count | 312 | 323 | 166 | 104 | 142 | 103 | 253 | 194 | 122 | 207 | 196 | 305 | 394 | 513 | 522 | 554 | 472 | 303 | 287 | 340 | 153 | 220 | 277 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 26.2 | 29.7 | 22.5 | 18.1 | 16.0 | 12.0 | 9.9 | 9.7 | 9.5 | 8.1 | 8.6 | 8.7 | 26.6 | 23.3 | 22.4 | 29.6 | 36.9 | 31.7 | 22.1 | 19.8 | 20.7 |
Std Deviation (1e-4) | 8.3 | 8.2 | 6.6 | 6.3 | 5.9 | 5.0 | 3.2 | 3.1 | 2.7 | 2.4 | 2.5 | 2.5 | 10.7 | 9.7 | 10.2 | 13.7 | 16.8 | 14.3 | 6.5 | 5.7 | 6.1 |
Intensity Change (Log2) | – | – | – | -0.31 | -0.49 | -0.9 | -1.18 | -1.22 | -1.24 | -1.47 | -1.39 | -1.38 | 0.24 | 0.05 | -0.0 | 0.4 | 0.72 | 0.5 | -0.02 | -0.18 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 0 | 1.4 | 2.4 | 3.0 | 2.0 | 2.5 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.7 | 0 | 4.0 | 2.8 | 1.9 | 2.7 | 3.0 | 3.6 | 2.6 | 3.6 | 3.5 | 1.7 | -1.7 | -0.9 | -0.2 |
Nucleus | -3.7 | -4.3 | -1.9 | -0.1 | 1.0 | -1.4 | 0.8 | -1.6 | 2.2 | -6.8 | -10.2 | -11.0 | 0.6 | 0.2 | 2.0 |
Nuclear Periphery | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.7 | 2.5 | 0.3 | -0.3 | -2.1 | 0.3 | -1.3 | 0.5 | -2.8 | 5.5 | 6.5 | 5.6 | -3.1 | -2.7 | -2.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 1.8 | 0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.4496 | 28.2283 | 26.7992 | 25.8131 | 24.0027 | 27.6566 | 22.5189 | 27.3524 | 25.1957 | 24.2928 | 22.2788 | 24.6331 | 22.2696 | 25.376 | 28.0285 | 20.8166 | 22.3745 | 24.6529 |
Actin | 0.0138 | 0.0001 | 0.0005 | 0.0002 | 0.042 | 0.0002 | 0.006 | 0.0004 | 0.0063 | 0 | 0.0323 | 0.0001 | 0 | 0.0163 | 0 | 0.0421 | 0 | 0.0001 |
Bud | 0.0011 | 0.0009 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0001 |
Bud Neck | 0.002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0029 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0017 | 0.0008 | 0.0002 | 0.0004 | 0.0008 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0014 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0004 |
Bud Site | 0.0074 | 0.0013 | 0.0003 | 0.0006 | 0.0009 | 0.0001 | 0.0006 | 0.0005 | 0.0013 | 0.0001 | 0.0018 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0019 | 0.0001 | 0.0001 |
Cell Periphery | 0.0028 | 0.0011 | 0.0005 | 0.0007 | 0.0004 | 0.0008 | 0.0005 | 0.0001 | 0.0004 | 0.0004 | 0.0007 | 0.0009 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0005 |
Cytoplasm | 0.0012 | 0.0001 | 0.0176 | 0.0009 | 0.0001 | 0.0012 | 0.0042 | 0.0004 | 0.0006 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0 | 0.0106 | 0 | 0 |
Cytoplasmic Foci | 0.0233 | 0.0023 | 0.002 | 0.0014 | 0.0027 | 0.0034 | 0.0167 | 0.0046 | 0.0005 | 0.0002 | 0.0193 | 0.0001 | 0.0067 | 0.0039 | 0 | 0.0082 | 0 | 0 |
Eisosomes | 0.0004 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0 |
Endoplasmic Reticulum | 0.0005 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0022 | 0 | 0.0017 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0.0071 | 0 | 0 |
Endosome | 0.0021 | 0.0002 | 0.009 | 0.0002 | 0.0045 | 0.0009 | 0.0088 | 0.0003 | 0.0046 | 0.0001 | 0.0043 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0231 | 0 | 0 |
Golgi | 0.0011 | 0 | 0.0031 | 0.0001 | 0.0196 | 0.0001 | 0.0012 | 0.0005 | 0.0022 | 0 | 0.0034 | 0 | 0 | 0.0043 | 0 | 0.0048 | 0 | 0 |
Lipid Particles | 0.0115 | 0.0007 | 0.0006 | 0.0016 | 0.0052 | 0.0015 | 0.0094 | 0.0002 | 0.0006 | 0.0007 | 0.0073 | 0.0009 | 0.0009 | 0.0304 | 0.0002 | 0.007 | 0 | 0.0005 |
Mitochondria | 0.0112 | 0.0079 | 0.0072 | 0.011 | 0.0072 | 0.0031 | 0.0065 | 0.0008 | 0.0024 | 0.0013 | 0.0134 | 0.0035 | 0.0044 | 0.0154 | 0.001 | 0.0026 | 0.0015 | 0.0189 |
None | 0.0039 | 0.0002 | 0.0003 | 0.0008 | 0.0001 | 0.0057 | 0.0044 | 0 | 0.0002 | 0.0001 | 0.0028 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0151 | 0 | 0 |
Nuclear Periphery | 0.0049 | 0.0144 | 0.0011 | 0.0047 | 0.0328 | 0.0099 | 0.009 | 0.0032 | 0.0036 | 0.0168 | 0.0359 | 0.0134 | 0.0013 | 0.0017 | 0.0018 | 0.0478 | 0.0005 | 0.0053 |
Nucleolus | 0.5709 | 0.4151 | 0.6703 | 0.5792 | 0.4701 | 0.5724 | 0.5796 | 0.6273 | 0.7121 | 0.6094 | 0.4715 | 0.668 | 0.779 | 0.7015 | 0.8157 | 0.3962 | 0.4459 | 0.7193 |
Nucleus | 0.2907 | 0.5399 | 0.2832 | 0.3853 | 0.3983 | 0.3766 | 0.3293 | 0.3558 | 0.2521 | 0.3523 | 0.3827 | 0.2942 | 0.2001 | 0.2149 | 0.1758 | 0.4115 | 0.5504 | 0.2466 |
Peroxisomes | 0.0011 | 0.0006 | 0.0004 | 0.0004 | 0.0017 | 0.0001 | 0.0011 | 0.0035 | 0.0001 | 0 | 0.0021 | 0 | 0.0002 | 0.002 | 0 | 0.0027 | 0 | 0 |
Punctate Nuclear | 0.0037 | 0.0006 | 0.0004 | 0.0012 | 0.0005 | 0.0008 | 0.0146 | 0.0012 | 0.0004 | 0.0005 | 0.0032 | 0.0006 | 0.001 | 0.0017 | 0.0002 | 0.0108 | 0.0001 | 0.0006 |
Vacuole | 0.0107 | 0.0036 | 0.0012 | 0.0025 | 0.0017 | 0.0046 | 0.0018 | 0.0002 | 0.0024 | 0.0033 | 0.0039 | 0.0028 | 0.0019 | 0.0011 | 0.0009 | 0.0016 | 0.0004 | 0.0015 |
Vacuole Periphery | 0.0338 | 0.0099 | 0.0013 | 0.0085 | 0.01 | 0.0177 | 0.0031 | 0.0009 | 0.0054 | 0.0144 | 0.0111 | 0.0145 | 0.0026 | 0.0048 | 0.0037 | 0.0041 | 0.0006 | 0.0059 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 122.1299 | 126.8468 | 108.1745 | 76.9012 | 90.1569 | 83.9619 | 89.0126 | 68.0448 | 56.7627 | 88.1251 |
Translational Efficiency | 1.8817 | 1.9787 | 1.5611 | 1.8485 | 1.8723 | 1.818 | 1.7279 | 1.6514 | 1.5312 | 1.7127 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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