Standard name
Description Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B'

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.07 0 0.07 0 0 0 0.15 0.15 0.08 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0 0.05 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.93 0.95 0.97 0.94 0.88 0.87 0.88 0.82 0.83 0.84 0.94 0.85 0.78 0.8 0.87 0.87 0.9 0.92 0.93 0.84 0.75 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.11 0 0 0 0.05 0.06 0.12 0.11 0.2 0.16 0 0 0.06 0.05 0.05 0.06 0 0 0 0 0.1 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0.06 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0
Bud 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 5 0 3 3 6 11 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 2 6 6 8 16 26 8 19 10 13 10 43 52 15 7 6 0 0 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 3 0 0 0 0 1 0
Endosome 0 0 0 0 4 1 0 0 0 0 0 0 0 2 1 0 1 3 2 0 2 0 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 1 2 3
Mitochondria 0 4 0 3 0 46 3 14 10 20 20 1 0 0 6 1 1 0 0 2 1 2 2
Nucleus 169 96 179 231 298 281 333 246 216 208 318 222 246 265 142 104 94 165 98 178 175 207 226
Nuclear Periphery 1 0 0 0 11 5 11 3 8 2 5 0 0 3 0 0 0 1 0 0 0 1 1
Nucleolus 13 11 2 7 11 17 24 34 29 50 62 9 9 19 8 6 6 3 1 3 8 27 32
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 3 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 1 1 0 0 5 0 0 1 1 0 0 0 0 4
Vac/Vac Membrane 1 0 3 1 3 3 6 3 10 1 5 0 1 4 14 2 4 5 2 3 12 26 42
Unique Cell Count 176 103 189 239 318 320 381 279 263 250 379 235 291 339 177 120 108 183 107 193 208 278 325
Labelled Cell Count 187 113 190 248 335 374 405 313 297 298 437 242 299 355 189 121 117 183 107 193 208 278 325


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 8.4 9.2 8.4 9.3 6.1 8.0 7.1 8.9 7.2 7.3 10.3 10.5 10.6 12.9 13.0 13.7 9.1 9.5 9.7
Std Deviation (1e-4) 2.0 2.2 2.8 2.2 2.0 1.6 2.0 1.6 10.6 1.7 2.5 2.3 4.2 3.5 3.5 3.6 3.8 2.2 2.2 2.1
Intensity Change (Log2) -0.13 0.01 -0.59 -0.2 -0.38 -0.05 -0.37 -0.33 0.16 0.18 0.2 0.48 0.5 0.57 -0.02 0.04 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -0.4 1.0 1.8 -0.2 1.9 0.5 0.2 0.6 4.1 4.3 2.2 1.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.5 0 3.1 0 4.0 3.2 0 0 0 0 0 0
Nucleus 1.0 -0.5 -2.5 -2.7 -2.4 -4.0 -3.7 -3.7 -0.1 -3.4 -5.0 -4.2 -2.5 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.4 2.8 4.4 4.1 6.1 5.4 0 0 2.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 1.4 0 0 0 0 0 2.9 0 0