Standard name | |
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Description | ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p; human homolog ABCF2 can complement yeast ARB1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 6 | 2 | 4 | 3 | 8 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 5 | 4 | 0 | 3 | 2 | 12 | 18 | 5 | 9 | 6 | 9 | – | – | 1 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 351 | 314 | 103 | 79 | 149 | 73 | 114 | 137 | 71 | 111 | 110 | 84 | – | – | 19 | 260 | 256 | 260 | 333 | 292 | 87 | 5 | 34 | 41 |
Endoplasmic Reticulum | 3 | 11 | 2 | 0 | 8 | 8 | 19 | 28 | 13 | 13 | 9 | 10 | – | – | 0 | 8 | 6 | 9 | 3 | 2 | 2 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 0 | 5 | – | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 7 | 6 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 4 | 0 | 2 | – | – | 0 | 3 | 3 | 3 | 0 | 2 | 0 | 0 | 0 | 1 |
Unique Cell Count | 354 | 320 | 104 | 79 | 157 | 81 | 141 | 178 | 94 | 136 | 126 | 112 | 20 | 264 | 257 | 264 | 342 | 305 | 96 | 9 | 40 | 49 | ||
Labelled Cell Count | 356 | 333 | 109 | 79 | 161 | 84 | 152 | 191 | 101 | 145 | 135 | 125 | 21 | 275 | 269 | 275 | 342 | 305 | 96 | 9 | 40 | 49 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 56.0 | 69.5 | 55.0 | 48.6 | 45.9 | 35.5 | 33.8 | 33.2 | 29.8 | 27.6 | 27.0 | 26.8 | – | – | 63.2 | 75.6 | 73.3 | 70.3 | – | 52.2 | 46.8 |
Std Deviation (1e-4) | 10.0 | 17.1 | 13.6 | 13.0 | 9.3 | 7.4 | 8.5 | 7.9 | 7.9 | 5.5 | 5.8 | 6.3 | – | – | 24.6 | 23.0 | 20.5 | 20.8 | – | 24.8 | 28.5 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.26 | -0.63 | -0.7 | -0.73 | -0.88 | -0.99 | -1.03 | -1.04 | – | – | 0.2 | 0.46 | 0.42 | 0.35 | – | -0.08 | -0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.5 | 1.9 | 0 | 0.9 | 0 | 1.3 | – | – | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | -1.8 | -2.8 | -4.4 | -5.0 | -5.1 | -4.3 | -3.4 | -5.2 | – | – | -1.3 | -0.4 | 0.7 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.2 | 3.6 | 3.2 | 2.4 | 0 | 2.2 | – | – | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |