Standard name
Description ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p; human homolog ABCF2 can complement yeast ARB1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.07 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.09 0.1 0.05 0.07 0.05 0.08 – – 0.05 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 1.0 0.95 0.9 0.81 0.77 0.76 0.82 0.87 0.75 – – 0.95 0.98 1.0 0.98 0.97 0.96 0.91 0.6 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0.05 0.1 0.13 0.16 0.14 0.1 0.07 0.09 – – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0.05 – – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0.22 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 5 6 2 4 3 8 – – 0 1 0 0 0 0 1 0 0 0
Bud 0 1 0 0 0 0 0 0 1 0 0 1 – – 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 2 5 4 0 3 2 12 18 5 9 6 9 – – 1 2 4 2 0 0 0 0 0 0
Cytoplasm 351 314 103 79 149 73 114 137 71 111 110 84 – – 19 260 256 260 333 292 87 5 34 41
Endoplasmic Reticulum 3 11 2 0 8 8 19 28 13 13 9 10 – – 0 8 6 9 3 2 2 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – 1 0 0 0 0 0 0 0 0 0
Golgi 0 1 0 0 0 0 0 1 4 4 0 5 – – 0 1 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1 1 3 0 7 6 – – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1 0 0 0 0 1 0 0 0 0 0 – – 0 0 0 0 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 1 1 0 0 2 4 0 2 – – 0 3 3 3 0 2 0 0 0 1
Unique Cell Count 354 320 104 79 157 81 141 178 94 136 126 112 20 264 257 264 342 305 96 9 40 49
Labelled Cell Count 356 333 109 79 161 84 152 191 101 145 135 125 21 275 269 275 342 305 96 9 40 49


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 56.0 69.5 55.0 48.6 45.9 35.5 33.8 33.2 29.8 27.6 27.0 26.8 – – 63.2 75.6 73.3 70.3 – 52.2 46.8
Std Deviation (1e-4) 10.0 17.1 13.6 13.0 9.3 7.4 8.5 7.9 7.9 5.5 5.8 6.3 – – 24.6 23.0 20.5 20.8 – 24.8 28.5
Intensity Change (Log2) – – – -0.18 -0.26 -0.63 -0.7 -0.73 -0.88 -0.99 -1.03 -1.04 – – 0.2 0.46 0.42 0.35 – -0.08 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cell Periphery 0 0 0 1.5 1.9 0 0.9 0 1.3 – – 0 0 0 0
Cytoplasm 0.9 -1.8 -2.8 -4.4 -5.0 -5.1 -4.3 -3.4 -5.2 – – -1.3 -0.4 0.7 -0.4
Endoplasmic Reticulum 0 0 0 3.2 3.6 3.2 2.4 0 2.2 – – 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – 0 0 0 0