Standard name
Description S-adenosyl-L-homocysteine hydrolase; catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor; regulates cellular lipid homoeostasis by regulating phosphatidylcholine(PC)synthesis and triacylglycerol (TG) levels

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.28 0.42 0.34
Bud Site 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.96 0.95 0.92 0.41 0.31 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0 0.17
Nucleus 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.13 0.06 0 0
Nucleolus 0 0 0 0 0 0.09 0 0.14
Peroxisomes 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.37 0.29
Vac/Vac Membrane 0 0 0 0 0.14 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 1
Bud Site 0 0 0
Cell Periphery 10 9 5 0 0 0 0 0
Cytoplasm 227 268 158 31 1 0 0 0
Endoplasmic Reticulum 11 11 7 0 0 0 0 0
Endosome 0 1 0 0 0 0 0 0
Golgi 5 3 0 0 0 0 0 0
Mitochondria 1 3 1 0 0 0 0 0
Nucleus 0 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 3 3 0 0 0 0 0
Unique Cell Count 245 278 167 34 3 3 1 3
Labelled Cell Count 260 298 175 34 3 3 1 3


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 84.1 86.2 91.6
Std Deviation (1e-4) 27.7 27.1 30.8
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sah1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available