Standard name
Description Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis; exclusive to eukaryotes, implicated as eukaryotic supplement to the bacterium-derived Fe-S cluster (ISC) assembly apparatus; involved in regulation of iron metabolism; member of the LYR protein family

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.11 0.15 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0.11 0 0 0 0 0 0 0 0 0 0.39 0.34 0.07 0.12 0 0 0.49 0
Mitochondria 0.93 0.9 0.98 0.96 0.91 0.93 0.9 0.93 0.94 0.93 0.93 0 0.06 0.84 0.75 0.85 0.75 0.48 0.83
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0.05 0.06 0.05 0.06 0.08 0.06 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.06 0.11 0.06 0.13 0.18 0.18 0.13 0.17 0.17 0.15 0.17 0.15 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0.06 0.19 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.1 0 0 0 0 0 0 0 0 0 0.11 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 6 7 6 1 0 2 0 1 0 6 2 7 15 15 6 0 0 0
Bud 1 0 13 6 5 4 4 5 5 5 17 0 2 1 3 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
Bud Site 1 0 3 0 0 0 1 1 4 3 5 1 0
Cell Periphery 4 11 3 7 1 0 1 2 6 0 2 2 5 0 0 0 0 0 0
Cytoplasm 0 5 1 1 3 0 6 7 0 2 4 1 1 0 1 0 0 0 0
Endoplasmic Reticulum 1 1 0 3 0 0 0 2 1 2 3 0 1 0 0 0 0 0 0
Endosome 0 0 0 0 2 1 0 1 0 0 1 1 2 7 1 5 0 0 0
Golgi 21 43 1 0 3 1 0 2 3 1 0 7 16 46 46 17 0 2 0
Mitochondria 571 346 409 372 351 402 500 461 548 356 555 0 3 543 302 353 2 2 9
Nucleus 2 1 1 0 0 2 0 3 0 0 3 0 0 0 0 0 0 0 0
Nuclear Periphery 19 8 20 17 16 21 34 27 36 32 37 0 1 2 1 0 0 0 0
Nucleolus 46 23 48 23 52 77 103 67 96 66 92 3 7 4 7 1 0 0 0
Peroxisomes 9 1 9 3 6 6 3 5 7 4 11 1 4 7 6 26 0 0 0
SpindlePole 0 1 2 1 2 2 1 1 3 4 2 0 1 1 1 1 0 0 0
Vac/Vac Membrane 47 38 5 2 13 4 13 13 13 5 6 2 4 11 14 2 0 0 0
Unique Cell Count 617 386 418 387 386 433 557 497 581 381 597 18 47 646 404 417 4 5 11
Labelled Cell Count 729 484 522 441 455 520 668 597 723 481 744 20 54 646 404 417 4 5 11


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 26.7 14.8 20.8 21.4 19.9 19.3 20.1 19.7 18.2 18.0 23.4 22.1
Std Deviation (1e-4) 4.6 6.3 4.2 5.9 5.9 5.8 5.6 6.4 5.7 5.8 5.5 7.5 7.2
Intensity Change (Log2) 0.49 0.53 0.43 0.39 0.44 0.41 0.3 0.29 0.66 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 0 0 0 0 0 0 -0.9 0 0
Bud -1.5 -1.7 -2.3 -2.8 -2.3 -2.6 -1.7 -0.2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.4 -4.3 -3.4 -5.0 -3.5 -2.7 -3.1 -3.5 -16.9 -18.7
Nucleus 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.3 -0.4 0 0.9 0.4 1.0 2.1 1.0 0 0
Nucleolus -2.8 0.9 2.6 3.0 0.9 2.2 2.4 1.8 0 0.7
Peroxisomes -1.6 -0.6 -0.9 -2.3 -1.4 -1.2 -1.2 -0.4 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.1 0 1.3 1.5 1.2 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isd11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available