ORF
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0.05 0.05 0.07 0.07 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.06 0.08 0.1 0.07 0.13 0.17 0.12 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.59 0.33 0.73 0.77 0.83 0.69 – 0.41 0.37 0.3 0.28 0.26 0.49 0.3 0.25 0.77 0.85 0.82 0.19 0 0.24 0.26 0.28 0.39
Endoplasmic Reticulum 0.39 0.68 0.12 0.09 0.05 0.08 – 0.14 0.15 0.22 0.26 0.22 0.39 0.61 0.59 0 0.07 0 0.63 0.84 0.46 0.47 0.43 0.31
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.11 0.2 0.07 0.3 – 0.25 0.44 0.37 0.38 0.42 0 0 0 0 0 0 0 0 0.09 0.09 0.06 0.06
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.06 0 0 0 0 – 0.23 0.18 0.17 0.16 0.18 0.08 0 0.09 0.09 0.06 0.09 0.07 0 0.1 0.09 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 2 1 6 6 4 – 9 12 21 17 24 19 34 44 4 4 6 4 1 5 2 9 11
Bud 4 0 5 11 6 10 – 31 20 30 22 24 10 7 3 0 0 0 6 0 4 2 22 16
Bud Neck 0 0 1 0 0 0 – 3 0 0 0 1 0 0 0 0 0 0 6 0 0 0 6 9
Bud Site 0 0 0 0 0 0 – 1 2 0 1 3 2 1 5 0 0 0 – – – – – –
Cell Periphery 5 0 2 7 6 8 – 18 15 28 33 48 24 69 104 11 10 9 2 0 4 1 13 10
Cytoplasm 197 34 104 302 331 332 – 342 174 148 115 130 171 158 156 73 90 100 74 4 45 31 128 178
Endoplasmic Reticulum 130 70 17 34 19 40 – 116 69 107 109 111 136 317 362 1 7 3 254 97 87 57 192 142
Endosome 1 0 0 2 13 2 – 7 4 2 1 2 3 5 6 1 1 3 4 2 1 0 5 6
Golgi 0 0 0 0 0 1 – 0 0 2 1 0 3 3 10 2 0 0 1 0 0 1 5 1
Mitochondria 10 0 15 80 28 142 – 207 204 181 158 207 8 14 7 4 2 5 14 2 16 10 25 27
Nucleus 0 0 0 2 0 0 – 13 6 5 4 3 1 1 0 0 0 2 0 0 0 0 0 0
Nuclear Periphery 1 2 0 1 0 3 – 42 27 21 14 19 1 2 1 0 0 1 0 2 0 0 2 0
Nucleolus 0 0 0 0 0 0 – 3 0 1 3 0 0 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 0 1 0 4 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 – 5 2 2 1 2 10 3 16 0 0 0 1 0 0 0 2 2
Vac/Vac Membrane 21 6 2 6 13 18 – 195 85 85 68 89 28 17 56 9 6 11 26 3 19 11 29 44
Unique Cell Count 336 103 142 393 397 478 830 467 495 417 496 352 519 616 95 106 122 403 116 189 123 451 459
Labelled Cell Count 376 114 147 451 423 560 992 620 633 547 663 417 631 776 105 120 140 403 116 189 123 451 459


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 12.0 4.4 4.6 5.3 3.9 – 5.9 6.3 8.4 10.2 11.0 9.1 12.9 15.0 10.2 10.2 10.0 6.3 8.1 6.8
Std Deviation (1e-4) 2.0 4.5 1.2 1.8 4.0 1.3 – 3.0 4.1 6.5 8.3 9.2 4.7 7.3 8.3 3.6 2.9 3.3 2.2 15.1 8.5
Intensity Change (Log2) – – – 0.06 0.27 -0.18 – 0.43 0.52 0.95 1.23 1.33 1.05 1.56 1.78 1.22 1.22 1.19 0.52 0.89 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 2.2 2.4 2.8 2.9 0 0 0
Bud 0 0 0 – 0.1 0.4 1.2 0.8 0.7 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 2.1 2.8 3.2 2.4 4.1 4.8 3.4 2.9 2.4
Cytoplasm 0.9 2.6 -0.9 – -7.1 -7.5 -9.3 -9.6 -10.3 -5.0 -9.2 -10.8 0.6 2.2 1.7
Endoplasmic Reticulum 0 -2.9 0 – 0.6 0.8 2.6 3.5 2.7 5.8 10.4 10.1 -3.1 -1.4 -2.9
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -1.3 0 – 3.8 7.2 5.9 6.1 6.9 -4.0 -4.1 0 -1.8 -2.7 -2.0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 2.7 2.9 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 6.0 5.0 4.8 4.6 5.0 2.8 0 3.1 0 0 2.8