Standard name
Description Major isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; relative distribution to the nucleus increases upon DNA replication stress; RNR1 has a paralog, RNR3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.25 0.16 0.1 0.05 0.06 0.22 0.15 0.11 0.46 0.2 0.21 0.24 0.06 0 0 0 0 0 0
Cytoplasm 0.65 0.89 0.9 0.84 0.91 0.67 0.85 0.79 0.5 0.86 0.76 0.82 0.91 0.33 0.34 0.34 0.7 0.7 0.71
Endoplasmic Reticulum 0.05 0 0 0 0.06 0.06 0 0.05 0.12 0 0.06 0.12 0.09 0.07 0.12 0.06 0.08 0.1 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0.1 0.05 0.05 0 0 0.06 0 0.05 0 0 0 0 0 0.18 0.19 0.22 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.08 0.05 0 0
Nucleolus 0.05 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 2 1
Bud 1 0 0 0 0 1 0 0 1 2 0 0 0 3 2 4 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 3 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 10 3 2 1 2 4 5 2 12 10 7 4 2 0 0 1 1 0 0
Cytoplasm 26 17 19 16 29 12 28 15 13 42 25 14 31 18 23 33 29 85 83
Endoplasmic Reticulum 2 0 0 0 2 1 0 1 3 0 2 2 3 4 8 5 3 12 13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 1 0
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0
Nucleus 4 1 1 0 0 1 0 1 0 0 0 0 0 10 13 21 1 4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 4 7 8 2 1 1
Nucleolus 2 0 0 2 0 0 1 0 1 0 0 0 1 1 1 5 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 2 0 0 0 0 0 0 0 1 0 2 0 0 2 2 2 1 1 2
Unique Cell Count 40 19 21 19 32 18 33 19 26 49 33 17 34 56 69 97 43 122 118
Labelled Cell Count 47 21 22 19 33 20 34 19 31 54 36 20 37 56 69 97 43 122 118


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 206.4 165.8 174.7 164.5 160.3 212.5 170.6 162.2 280.6 294.6 297.3 213.0 211.3 198.4 245.0
Std Deviation (1e-4) 90.2 62.6 97.0 93.2 70.7 87.1 73.4 71.1 93.7 107.3 131.5 61.8 93.9 79.9 113.6
Intensity Change (Log2) -0.32 -0.24 -0.33 -0.36 0.04 -0.27 -0.35 0.44 0.51 0.53 0.05 0.03 -0.06 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 -2.1 0 -1.0 0 1.8 -0.5 -0.4 0 -2.2
Cytoplasm 2.0 2.1 1.5 2.5 0.1 1.9 1.1 -1.2 2.3 1.0 1.3 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available