Standard name
Description E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.11 0.08 0.11 0.09
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.05 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.06 0.15 0.09 0.05 0.17 0.15 0.06 0.29 0.25 0.19
Mitochondria 0.68 0.69 0.53 0.71 0.47 0.6 0.51 0.45 0.52 0.51 0.5 0.57 0.56 0.58 0.67 0.17 0.2 0.16 0.26 0.23 0.17 0.25 0.2 0.23
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.42 0.62 0.42 0.39 0.48 0.64 0.72 0.75 0.76 0.78 0.73 0.71 0.15 0.15 0.16 0.5 0.69 0.68 0 0.1 0.05 0.06 0.08 0.09
Peroxisomes 0.11 0 0.21 0.1 0.21 0.11 0.06 0.06 0 0 0.05 0 0.32 0.17 0.16 0.05 0.05 0.11 0.27 0.18 0.45 0.15 0.19 0.19
SpindlePole 0.08 0 0.1 0.06 0 0 0 0 0 0 0 0 0.13 0.13 0.09 0 0.05 0.05 0.09 0.1 0.09 0.05 0.08 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 4 0 0 1 0 3 1 1 0 0 0 7 2 32 12 29 20
Bud 0 0 0 1 0 3 4 4 1 1 2 0 0 1 0 0 0 0 0 0 2 2 3 4
Bud Neck 0 0 0 0 1 1 2 1 0 1 0 0 0 1 1 0 0 0 1 0 7 0 1 2
Bud Site 0 0 3 0 3 1 5 8 1 3 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 0 2 1 3 3 3 3 5 2 1 2 3 3 3 10 3 2 0 0 0 3 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 0 0 0 0 0 0
Endosome 1 0 2 0 3 5 1 3 0 1 0 0 1 3 2 0 1 0 2 3 4 4 5 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 19 10 10 6 1 14 7 15 42 66 46
Mitochondria 49 11 91 22 74 172 214 201 122 165 139 175 55 87 106 11 13 3 21 12 50 36 52 55
Nucleus 0 0 0 0 0 2 3 6 2 5 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2 1 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0
Nucleolus 30 10 72 12 75 183 302 336 179 250 201 219 15 23 26 33 44 13 2 5 14 9 21 21
Peroxisomes 8 0 37 3 33 32 26 29 7 10 15 12 32 26 25 3 3 2 22 9 128 22 50 44
SpindlePole 6 0 18 2 7 12 12 16 2 6 1 4 13 20 14 1 3 1 7 5 26 6 20 19
Vac/Vac Membrane 0 0 0 0 1 1 0 2 2 3 0 0 1 7 1 3 3 0 0 3 0 3 8 12
Unique Cell Count 72 16 173 31 157 285 418 451 235 322 277 307 99 150 159 66 64 19 82 54 287 147 267 240
Labelled Cell Count 97 21 225 41 200 419 575 614 321 447 364 416 124 191 190 73 77 23 82 54 287 147 267 240


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.5 18.4 30.5 29.0 28.7 29.9 34.2 37.3 36.7 40.4 39.2 38.7 32.2 31.8 32.5 45.3 42.2 39.6 30.9 34.1 36.8
Std Deviation (1e-4) 10.8 6.0 13.2 13.7 13.3 16.1 18.4 21.1 20.6 23.4 21.6 20.5 15.0 13.8 12.0 21.3 18.2 15.0 12.3 14.8 16.3
Intensity Change (Log2) – – – -0.08 -0.09 -0.03 0.16 0.29 0.27 0.4 0.36 0.34 0.08 0.06 0.09 0.57 0.47 0.37 0.02 0.16 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 4.8 0 0 0 0
Mitochondria 1.9 -1.0 1.6 -0.3 -1.8 -0.1 -0.3 -0.5 0.9 0.5 1.0 2.6 -5.0 -4.4 -3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 1.1 4.7 7.0 7.7 7.1 8.0 6.5 6.4 -4.5 -5.2 -5.0 1.2 3.7 2.2
Peroxisomes -1.5 -0.1 -2.9 -5.4 -5.4 -5.9 -6.6 -5.2 -6.1 2.0 -0.9 -1.3 -3.1 -3.0 0
SpindlePole 0 -2.0 -2.6 -3.8 -3.4 -4.4 -4.2 -5.2 -4.6 0.7 0.8 -0.5 -2.3 -1.4 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pda1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available