Standard name | |
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Description | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 7 | 2 | 32 | 12 | 29 | 20 |
Bud | 0 | 0 | 0 | 1 | 0 | 3 | 4 | 4 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 7 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 3 | 0 | 3 | 1 | 5 | 8 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 0 | 2 | 1 | 3 | 3 | 3 | 3 | 5 | 2 | 1 | 2 | 3 | 3 | 3 | 10 | 3 | 2 | 0 | 0 | 0 | 3 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 0 | 2 | 0 | 3 | 5 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 3 | 2 | 0 | 1 | 0 | 2 | 3 | 4 | 4 | 5 | 8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 19 | 10 | 10 | 6 | 1 | 14 | 7 | 15 | 42 | 66 | 46 |
Mitochondria | 49 | 11 | 91 | 22 | 74 | 172 | 214 | 201 | 122 | 165 | 139 | 175 | 55 | 87 | 106 | 11 | 13 | 3 | 21 | 12 | 50 | 36 | 52 | 55 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 6 | 2 | 5 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 30 | 10 | 72 | 12 | 75 | 183 | 302 | 336 | 179 | 250 | 201 | 219 | 15 | 23 | 26 | 33 | 44 | 13 | 2 | 5 | 14 | 9 | 21 | 21 |
Peroxisomes | 8 | 0 | 37 | 3 | 33 | 32 | 26 | 29 | 7 | 10 | 15 | 12 | 32 | 26 | 25 | 3 | 3 | 2 | 22 | 9 | 128 | 22 | 50 | 44 |
SpindlePole | 6 | 0 | 18 | 2 | 7 | 12 | 12 | 16 | 2 | 6 | 1 | 4 | 13 | 20 | 14 | 1 | 3 | 1 | 7 | 5 | 26 | 6 | 20 | 19 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 3 | 0 | 0 | 1 | 7 | 1 | 3 | 3 | 0 | 0 | 3 | 0 | 3 | 8 | 12 |
Unique Cell Count | 72 | 16 | 173 | 31 | 157 | 285 | 418 | 451 | 235 | 322 | 277 | 307 | 99 | 150 | 159 | 66 | 64 | 19 | 82 | 54 | 287 | 147 | 267 | 240 |
Labelled Cell Count | 97 | 21 | 225 | 41 | 200 | 419 | 575 | 614 | 321 | 447 | 364 | 416 | 124 | 191 | 190 | 73 | 77 | 23 | 82 | 54 | 287 | 147 | 267 | 240 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 23.5 | 18.4 | 30.5 | 29.0 | 28.7 | 29.9 | 34.2 | 37.3 | 36.7 | 40.4 | 39.2 | 38.7 | 32.2 | 31.8 | 32.5 | 45.3 | 42.2 | 39.6 | 30.9 | 34.1 | 36.8 |
Std Deviation (1e-4) | 10.8 | 6.0 | 13.2 | 13.7 | 13.3 | 16.1 | 18.4 | 21.1 | 20.6 | 23.4 | 21.6 | 20.5 | 15.0 | 13.8 | 12.0 | 21.3 | 18.2 | 15.0 | 12.3 | 14.8 | 16.3 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.09 | -0.03 | 0.16 | 0.29 | 0.27 | 0.4 | 0.36 | 0.34 | 0.08 | 0.06 | 0.09 | 0.57 | 0.47 | 0.37 | 0.02 | 0.16 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 | 0 | 0 |
Mitochondria | 1.9 | -1.0 | 1.6 | -0.3 | -1.8 | -0.1 | -0.3 | -0.5 | 0.9 | 0.5 | 1.0 | 2.6 | -5.0 | -4.4 | -3.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.3 | 1.1 | 4.7 | 7.0 | 7.7 | 7.1 | 8.0 | 6.5 | 6.4 | -4.5 | -5.2 | -5.0 | 1.2 | 3.7 | 2.2 |
Peroxisomes | -1.5 | -0.1 | -2.9 | -5.4 | -5.4 | -5.9 | -6.6 | -5.2 | -6.1 | 2.0 | -0.9 | -1.3 | -3.1 | -3.0 | 0 |
SpindlePole | 0 | -2.0 | -2.6 | -3.8 | -3.4 | -4.4 | -4.2 | -5.2 | -4.6 | 0.7 | 0.8 | -0.5 | -2.3 | -1.4 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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