Standard name
Human Ortholog
Description E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.11 0.08 0.11 0.09
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.05 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.06 0.15 0.09 0.05 0.17 0.15 0.06 0.29 0.25 0.19
Mitochondria 0.68 0.69 0.53 0.71 0.47 0.6 0.51 0.45 0.52 0.51 0.5 0.57 0.56 0.58 0.67 0.17 0.2 0.16 0.26 0.23 0.17 0.25 0.2 0.23
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.42 0.62 0.42 0.39 0.48 0.64 0.72 0.75 0.76 0.78 0.73 0.71 0.15 0.15 0.16 0.5 0.69 0.68 0 0.1 0.05 0.06 0.08 0.09
Peroxisomes 0.11 0 0.21 0.1 0.21 0.11 0.06 0.06 0 0 0.05 0 0.32 0.17 0.16 0.05 0.05 0.11 0.27 0.18 0.45 0.15 0.19 0.19
SpindlePole 0.08 0 0.1 0.06 0 0 0 0 0 0 0 0 0.13 0.13 0.09 0 0.05 0.05 0.09 0.1 0.09 0.05 0.08 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 4 0 0 1 0 3 1 1 0 0 0 7 2 32 12 29 20
Bud 0 0 0 1 0 3 4 4 1 1 2 0 0 1 0 0 0 0 0 0 2 2 3 4
Bud Neck 0 0 0 0 1 1 2 1 0 1 0 0 0 1 1 0 0 0 1 0 7 0 1 2
Bud Site 0 0 3 0 3 1 5 8 1 3 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 0 2 1 3 3 3 3 5 2 1 2 3 3 3 10 3 2 0 0 0 3 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 0 0 0 0 0 0
Endosome 1 0 2 0 3 5 1 3 0 1 0 0 1 3 2 0 1 0 2 3 4 4 5 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 19 10 10 6 1 14 7 15 42 66 46
Mitochondria 49 11 91 22 74 172 214 201 122 165 139 175 55 87 106 11 13 3 21 12 50 36 52 55
Nucleus 0 0 0 0 0 2 3 6 2 5 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2 1 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0
Nucleolus 30 10 72 12 75 183 302 336 179 250 201 219 15 23 26 33 44 13 2 5 14 9 21 21
Peroxisomes 8 0 37 3 33 32 26 29 7 10 15 12 32 26 25 3 3 2 22 9 128 22 50 44
SpindlePole 6 0 18 2 7 12 12 16 2 6 1 4 13 20 14 1 3 1 7 5 26 6 20 19
Vac/Vac Membrane 0 0 0 0 1 1 0 2 2 3 0 0 1 7 1 3 3 0 0 3 0 3 8 12
Unique Cell Count 72 16 173 31 157 285 418 451 235 322 277 307 99 150 159 66 64 19 82 54 287 147 267 240
Labelled Cell Count 97 21 225 41 200 419 575 614 321 447 364 416 124 191 190 73 77 23 82 54 287 147 267 240


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.5 18.4 30.5 29.0 28.7 29.9 34.2 37.3 36.7 40.4 39.2 38.7 32.2 31.8 32.5 45.3 42.2 39.6 30.9 34.1 36.8
Std Deviation (1e-4) 10.8 6.0 13.2 13.7 13.3 16.1 18.4 21.1 20.6 23.4 21.6 20.5 15.0 13.8 12.0 21.3 18.2 15.0 12.3 14.8 16.3
Intensity Change (Log2) – – – -0.08 -0.09 -0.03 0.16 0.29 0.27 0.4 0.36 0.34 0.08 0.06 0.09 0.57 0.47 0.37 0.02 0.16 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 4.8 0 0 0 0
Mitochondria 1.9 -1.0 1.6 -0.3 -1.8 -0.1 -0.3 -0.5 0.9 0.5 1.0 2.6 -5.0 -4.4 -3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 1.1 4.7 7.0 7.7 7.1 8.0 6.5 6.4 -4.5 -5.2 -5.0 1.2 3.7 2.2
Peroxisomes -1.5 -0.1 -2.9 -5.4 -5.4 -5.9 -6.6 -5.2 -6.1 2.0 -0.9 -1.3 -3.1 -3.0 0
SpindlePole 0 -2.0 -2.6 -3.8 -3.4 -4.4 -4.2 -5.2 -4.6 0.7 0.8 -0.5 -2.3 -1.4 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 51.0546 80.6871 68.4133 68.4764 74.1109 72.0747 42.0362 87.9063 68.7697 71.3378 78.5379 73.3739 47.1731 75.1659 70.3585 65.1208 79.5454 66.8448
Actin 0.0044 0.0014 0.0017 0.0002 0.0002 0.0006 0.0086 0.0056 0.0031 0.0003 0.0003 0.001 0.0191 0.0073 0.0026 0.0004 0.0003 0.0003
Bud 0.0002 0.0001 0.0001 0.0001 0 0 0.0004 0.0005 0.0003 0 0 0.0001 0.0001 0.0001 0.0003 0.0001 0 0
Bud Neck 0.0003 0.0005 0.0022 0.0002 0 0.0001 0.002 0.0035 0.0012 0.0001 0.0001 0.0002 0.0004 0.0002 0.0003 0.0004 0.0001 0.0001
Bud Periphery 0.0005 0.0001 0 0 0 0 0.0004 0.0003 0.0005 0 0 0.0001 0.0001 0 0.0001 0.0002 0 0
Bud Site 0.0015 0.0016 0.0008 0.0007 0.0002 0.0001 0.0069 0.0108 0.003 0.0001 0.0001 0.0006 0.001 0.0007 0.0043 0.0005 0.0001 0
Cell Periphery 0.0006 0.0006 0.0006 0.0001 0.0001 0.0001 0.0014 0.0017 0.0015 0.0007 0.0002 0.0004 0.0005 0.0005 0.0004 0.0019 0.0001 0.0004
Cytoplasm 0.0006 0 0 0 0 0 0.0005 0 0.0002 0 0 0 0.0002 0 0 0 0 0
Cytoplasmic Foci 0.0354 0.0258 0.0186 0.0159 0.0182 0.0358 0.096 0.0419 0.0328 0.0492 0.005 0.0198 0.0268 0.0302 0.0285 0.067 0.0052 0.0021
Eisosomes 0.0011 0.0004 0.0008 0.0002 0.0003 0.0003 0.0017 0.0011 0.0014 0.0004 0.0003 0.0007 0.0014 0.0008 0.0006 0.0006 0.0003 0.0004
Endoplasmic Reticulum 0.0005 0 0 0 0 0 0.0001 0 0.0002 0 0 0 0.0004 0 0 0 0 0
Endosome 0.0017 0.0002 0.0001 0 0 0.0001 0.0021 0.0006 0.0011 0.0001 0 0.0009 0.0047 0.0003 0.0003 0.0003 0 0.0001
Golgi 0.0017 0.0005 0.0002 0.0001 0.0001 0.0004 0.0052 0.0043 0.0019 0.0003 0.0002 0.0023 0.0033 0.0026 0.0032 0.0005 0.0002 0.0005
Lipid Particles 0.0589 0.0463 0.0211 0.0227 0.0129 0.0443 0.1176 0.0544 0.0473 0.0415 0.0247 0.0814 0.101 0.0647 0.0699 0.0776 0.0059 0.063
Mitochondria 0.7875 0.7744 0.8437 0.8304 0.8941 0.8425 0.5924 0.7037 0.7519 0.7482 0.8999 0.7964 0.7449 0.721 0.7826 0.7298 0.9252 0.8701
None 0.001 0.0001 0 0 0 0 0.0042 0.0001 0.0001 0 0 0 0.0005 0 0.0001 0.0001 0 0
Nuclear Periphery 0.0004 0.0053 0 0 0 0 0.0003 0.0004 0.0001 0.0001 0 0 0.0006 0.0001 0 0 0 0
Nucleolus 0.0206 0.0275 0.0269 0.0063 0.0244 0.0039 0.0534 0.0385 0.0345 0.0344 0.016 0.0262 0.013 0.0681 0.0196 0.029 0.0027 0.0167
Nucleus 0.0008 0.0011 0.0008 0.0001 0.0002 0.0001 0.002 0.0017 0.001 0.0009 0.0004 0.0007 0.0006 0.0019 0.0006 0.0015 0.0001 0.0007
Peroxisomes 0.0719 0.0997 0.0732 0.1224 0.0489 0.0665 0.0848 0.1099 0.1003 0.0955 0.0512 0.0603 0.0726 0.0909 0.0795 0.0662 0.0592 0.0317
Punctate Nuclear 0.0012 0 0 0 0 0 0.0012 0.0001 0.0026 0 0 0.0001 0.0006 0 0 0.0001 0 0
Vacuole 0.0021 0.0015 0.0019 0.0001 0.0001 0.0003 0.0034 0.004 0.0018 0.0032 0.0002 0.0008 0.0014 0.0016 0.0007 0.0054 0.0001 0.0012
Vacuole Periphery 0.0072 0.0131 0.0071 0.0003 0.0003 0.0048 0.0153 0.0169 0.0132 0.0249 0.001 0.0079 0.0067 0.0089 0.0061 0.0183 0.0006 0.0126

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 566.1172 558.3998 547.6463 571.6328 517.6482 490.7126 613.5556 577.4604 635.8259 576.2207
Translational Efficiency 1.2499 1.258 1.2506 1.3634 1.2536 1.3762 1.1383 1.2987 1.1038 1.0492

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes
Localization
Cell Percentages mitochondrion (91%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Pda1

Pda1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pda1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available