Standard name
Description Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells; human homolog ABCB1 mediates multidrug resistance in many chemotherapy-resistant tumors by effluxing toxic compounds from the cell

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0 0.11 0.06 0.1 0.11 0.18 0.2 0.17 0.23 0.42 0.73 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0.05 0.12 0 0.06 0 0 0 0 0 0 0.06 0 0 0 0.08 0.07 0.18 0.14 0.13
Golgi 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.05 0.05 0 0
Mitochondria 0.06 0.08 0.08 0 0 0.09 0.14 0.15 0.22 0.29 0.2 0 0 0 0 0 0.05 0.05 0.05 0
Nucleus 0.07 0.05 0.05 0.22 0.08 0.13 0.16 0.12 0.06 0.07 0.06 0.14 0.08 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.85 0.77 0.75 0.75 0.83 0.78 0.79 0.79 0.73 0.65 0.73 0.7 0.51 0.24 0.8 0.79 0.72 0.62 0.64 0.63
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 9 3 0 0 0 0 0 0 0 0 0 1 2 1 1 0 1 0 4
Bud 0 0 0 0 0 0 0 1 0 0 0 0 0 2 6 9 4 3 6 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 9 22 10 5 19 15 31 51 47 45 41 16 39 105 1 3 0 2 0 3
Endoplasmic Reticulum 2 10 5 0 0 0 0 2 1 0 0 2 7 3 3 5 6 1 2 3
Endosome 9 24 21 3 11 5 4 5 0 0 2 4 1 0 14 44 13 49 30 32
Golgi 21 14 7 1 0 0 0 0 0 0 1 5 0 1 12 13 9 12 6 10
Mitochondria 19 35 14 5 8 23 44 69 59 64 48 0 0 0 9 14 9 14 11 7
Nucleus 22 24 8 30 15 34 50 54 16 15 14 10 7 11 2 3 0 1 2 4
Nuclear Periphery 4 7 9 2 5 3 2 5 7 6 5 1 0 0 5 7 4 5 9 10
Nucleolus 9 23 10 1 2 3 2 4 3 3 5 0 0 0 6 3 1 2 3 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0
SpindlePole 1 0 0 0 1 0 0 0 0 0 1 0 1 0 1 1 0 1 0 2
Vac/Vac Membrane 253 339 131 104 149 197 250 362 193 146 172 50 47 34 271 412 135 167 139 156
Unique Cell Count 297 443 175 139 180 253 317 459 265 223 236 71 93 143 338 524 189 269 218 248
Labelled Cell Count 350 507 218 151 210 280 383 553 326 279 289 88 103 159 338 524 189 269 218 248


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.3 13.2 12.3 10.2 12.0 10.3 9.8 9.7 8.8 8.3 9.3 9.2 7.2 6.8 12.5 13.3 13.4
Std Deviation (1e-4) 4.5 4.3 3.8 3.7 4.8 4.6 4.5 4.0 3.6 3.4 3.5 2.2 1.4 1.4 3.5 3.7 3.8
Intensity Change (Log2) -0.28 -0.04 -0.26 -0.33 -0.35 -0.49 -0.58 -0.41 -0.43 -0.78 -0.86 0.02 0.11 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 1.7 0.1 1.6 2.1 3.7 4.2 3.5 3.9 7.3 12.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.3 -1.9 -4.3 -5.2 -6.2 -5.8 -5.3 -4.9 -1.5 -3.1 -4.3
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.6 -1.4 0.4 1.9 2.3 3.9 5.2 3.5 0 0 -3.5
Nucleus 4.6 1.4 3.0 3.7 2.7 0.7 0.9 0.6 2.6 1.0 1.2
Nuclear Periphery 0 -1.1 0 0 0 -1.4 -1.3 -1.7 0 0 0
Nucleolus 0 -2.4 -2.7 0 0 -2.8 -2.4 -1.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 0.7 1.0 1.1 -0.5 -2.0 -0.4 -0.7 -4.0 -9.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available