Standard name
Description Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.2 0.1 0.22 0.21 0.15 0.15 0.16 – 0.17 0.17 0.2 0.22 0.13 0.06 0.1 0.09 0.08 0 0.05 0.08 0.07 0.09 0.1
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.94 0.94 0.7 0.86 0.88 0.4 0.49 0.61 – 0.58 0.31 0.27 0.9 0.84 0.91 0.9 0.88 0.87 0.92 0.89 0.79 0.83 0.82 0.75
Mitochondria 0.38 0.17 0.71 0.58 0.37 0.83 0.68 0.56 – 0.61 0.83 0.83 0.36 0.26 0.25 0.16 0.09 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.06 0.07 0.05 0 0.07 0.09 0.1 – 0.09 0.07 0.07 0 0 0 0.12 0.09 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.05 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0.1 0.1 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 20 21 18 62 90 84 103 110 – 112 99 126 42 45 20 28 28 14 7 5 14 8 20 18
Bud 0 0 1 4 9 10 12 11 – 9 18 25 1 2 2 0 1 0 0 1 2 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 6 3 – 4 4 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 2 2 8 6 12 11 21 18 – 14 15 14 4 7 4 6 14 8 0 0 1 0 0 0
Endoplasmic Reticulum 0 3 7 7 4 9 4 7 – 4 6 7 9 6 3 11 12 10 0 0 3 1 1 5
Endosome 0 0 2 1 3 2 5 3 – 4 2 4 0 0 0 0 1 1 3 3 5 4 6 7
Golgi 225 101 133 236 385 225 329 422 – 374 177 172 168 284 297 257 268 158 231 114 147 95 186 134
Mitochondria 91 18 134 161 161 465 454 390 – 390 472 524 67 88 80 45 28 22 0 0 0 0 0 1
Nucleus 0 0 1 0 0 1 0 0 – 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0
Nuclear Periphery 0 0 1 0 1 0 0 0 – 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 16 6 13 13 16 40 61 66 – 55 40 46 7 11 14 34 27 16 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 10 6 – 7 2 4 0 1 0 0 0 0 3 0 6 3 5 2
SpindlePole 0 0 0 0 0 0 4 5 – 5 1 3 0 0 1 1 0 0 0 0 0 0 1 1
Vac/Vac Membrane 14 5 4 0 6 2 4 3 – 6 4 4 5 19 11 29 31 21 0 1 0 0 1 1
Unique Cell Count 240 107 189 276 439 557 669 692 643 572 628 187 338 325 284 305 182 252 128 186 115 227 179
Labelled Cell Count 368 156 322 490 687 849 1013 1044 986 841 934 304 463 432 411 411 251 252 128 186 115 227 179


Early Golgi, Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 51.5 55.3 29.2 30.5 31.0 26.2 26.4 27.2 – 27.3 25.1 24.6 45.1 48.2 46.9 53.4 54.0 54.2 48.5 48.5 43.4
Std Deviation (1e-4) 10.3 12.8 8.2 7.8 6.8 5.8 6.6 5.8 – 6.4 5.9 5.9 10.1 12.4 10.7 13.6 16.9 16.5 8.9 11.9 15.0
Intensity Change (Log2) – – – 0.06 0.09 -0.16 -0.14 -0.1 – -0.09 -0.22 -0.25 0.63 0.72 0.68 0.87 0.89 0.89 0.73 0.73 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.6 3.3 1.9 2.0 2.2 – 2.6 2.6 3.3 3.4 1.3 -1.4 0.1 -0.1 -0.6
Bud 0 0 0 0 0 – 0 0 2.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -1.0 0 -0.7 -1.2 – 0 -1.1 -1.5 -1.2 -1.4 0 -1.3 0.2 0.1
Endoplasmic Reticulum -0.7 0 0 0 0 – 0 0 0 0.5 0 0 0.1 0.1 0.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 4.0 5.2 -7.1 -5.2 -2.4 – -3.0 -9.6 -10.7 4.7 3.7 6.2 5.6 4.8 3.8
Mitochondria -2.8 -7.9 3.8 -0.8 -3.6 – -2.6 3.4 3.8 -6.8 -10.0 -10.3 -12.1 -14.2 -11.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus -1.0 -1.8 0.1 1.0 1.1 – 0.7 0.1 0.2 -1.4 -1.9 -1.3 1.8 0.8 0.7
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 1.9 0.8 3.4 3.4 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 38.65 49.5883 48.3585 47.2509 42.1074 51.3566 40.9015 50.374 49.5864 42.6758 41.7262 49.5083 46.939 55.8934 57.6718 51.9158 54.4905 54.1604
Actin 0.0856 0.039 0.0387 0.0185 0.0166 0.0355 0.0619 0.0319 0.0197 0.0182 0.0027 0.0154 0.0493 0.0129 0.0308 0.0052 0.0024 0.0107
Bud 0.0004 0.0003 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Bud Neck 0.0003 0.0001 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Periphery 0.0006 0.0004 0.0001 0.0003 0.0002 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Bud Site 0.001 0.0002 0.0003 0.0003 0.0001 0 0.0006 0.0001 0.0033 0.0001 0.0002 0 0.0003 0.0001 0.0001 0.0001 0 0
Cell Periphery 0.0004 0.0002 0.0002 0.0019 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.0022 0.0002 0.0003 0.0001 0.0009 0 0.0006 0.0001 0.0006 0.0001 0.0005 0 0.0005 0.0001 0.0005 0.0001 0.0001 0
Cytoplasmic Foci 0.0328 0.0153 0.0222 0.0095 0.0183 0.0125 0.0225 0.0128 0.0167 0.0468 0.0382 0.004 0.0207 0.011 0.01 0.0166 0.0054 0.0053
Eisosomes 0.0016 0.0003 0.0014 0.0025 0.0002 0.0002 0.0009 0.0002 0.0003 0.0004 0.0001 0.0002 0.0053 0.0005 0.0006 0.0002 0.0004 0.0003
Endoplasmic Reticulum 0.0015 0.0001 0.0004 0.0003 0.0003 0.0001 0.0003 0.0001 0.0008 0.0001 0.0004 0.0001 0.0005 0.0001 0.0026 0.0002 0.0002 0.0001
Endosome 0.0315 0.0259 0.0227 0.0189 0.0697 0.0106 0.0261 0.0194 0.0192 0.0122 0.0504 0.011 0.024 0.0292 0.0198 0.026 0.0195 0.0165
Golgi 0.6287 0.8485 0.7699 0.6463 0.735 0.8873 0.7868 0.8906 0.848 0.8154 0.7938 0.9478 0.7233 0.8294 0.7793 0.6882 0.825 0.9036
Lipid Particles 0.1055 0.0455 0.0797 0.2222 0.0711 0.0289 0.034 0.0246 0.0456 0.0491 0.0471 0.0157 0.0952 0.0705 0.0916 0.1812 0.1069 0.0324
Mitochondria 0.0661 0.0115 0.0314 0.049 0.0348 0.0141 0.0493 0.0131 0.0297 0.0367 0.0267 0.0037 0.0599 0.0347 0.0469 0.0358 0.0043 0.0239
None 0.0058 0.0007 0.0006 0.0001 0.0001 0 0.0006 0 0.0008 0.0002 0.0005 0 0.0012 0 0.0003 0.0003 0 0
Nuclear Periphery 0.0033 0.0001 0.0007 0.0006 0.0007 0.0001 0.0004 0 0.0004 0.0001 0.0005 0 0.0008 0.0001 0.0003 0.0008 0.0022 0.0001
Nucleolus 0.0029 0.0036 0.0007 0.0005 0.0005 0.0058 0.0008 0.001 0.0003 0.0005 0.0016 0 0.0022 0.0001 0.0013 0.0006 0.0001 0
Nucleus 0.002 0.0007 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0 0.0002 0 0.0003 0 0.0002 0.0001 0.0001 0
Peroxisomes 0.0112 0.0017 0.0086 0.0027 0.0052 0.0028 0.0089 0.0017 0.0051 0.0167 0.0152 0.0006 0.0046 0.0012 0.0021 0.0074 0.0002 0.0009
Punctate Nuclear 0.004 0.0002 0.0011 0.0001 0.0003 0.0001 0.0005 0 0.0001 0.0004 0.0027 0 0.0022 0 0.0011 0.0018 0 0
Vacuole 0.0023 0.0006 0.0033 0.0035 0.0111 0.0001 0.0009 0.0003 0.0011 0.0002 0.0042 0.0001 0.001 0.0007 0.0008 0.0025 0.0036 0.0003
Vacuole Periphery 0.0105 0.0048 0.0172 0.022 0.0343 0.0015 0.004 0.0038 0.0074 0.0024 0.0146 0.0013 0.008 0.009 0.0114 0.0326 0.0293 0.0056

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 225.7953 246.1077 160.9677 152.1184 230.6756 206.6379 199.9074 142.3921 132.1956 241.4187
Translational Efficiency 1.929 1.4982 1.4165 1.6078 1.586 2.0732 1.5356 1.5349 1.5997 1.4172

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘