Standard name
Description Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.21 0.32 0.21 0.39 0.18 0.13 0.13 0.15 0.12 0.1 0.11 0.18 0.27 0.35 0.35 0.35 0.15 0.12 0.2 0.08 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.14 0.1 0.18 0.29 0.4 0.27 0.37 0.39 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.64 0.63 0.53 0.63 0.36 0.52 0.41 0.46 0.43 0.42 0.48 0.81 0.7 0.5 0.51 0.49 0.44 0.56 0.67 0.44 0.62 0.5 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0.14 0.13 0.17 0.14 0.17 0.28 0.29 0.28 0.31 0.32 0.05 0.07 0.12 0.05 0.07 0.1 0.11 0 0.12 0.15 0.24 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.09 0 0 0.05 0 0.05 0.05 0.05 0 0 0 0.06 0.09 0.09 0.13 0.15 0 0 0.06 0.06 0.15 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1
Bud 2 0 1 3 1 5 7 6 7 10 14 1 0 0 0 0 0 18 5 6 6 4 1
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 2 0 0 1 2 1
Bud Site 0 0 0 0 1 7 3 6 5 11 6 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 3 1 3 1 1 1 1 2 0 0 1 7 0 0 0 0 0 0
Cytoplasm 53 9 36 27 75 44 56 50 58 41 50 31 78 120 70 75 88 34 8 26 13 4 1
Endoplasmic Reticulum 0 0 0 0 1 1 3 1 1 0 0 0 0 0 5 0 3 0 0 0 1 0 0
Endosome 11 0 2 2 5 0 1 6 2 0 0 3 8 20 8 4 4 5 0 6 0 10 20
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 1 1 3
Mitochondria 2 0 16 13 35 68 170 104 140 130 180 1 0 0 4 0 1 0 0 1 0 6 14
Nucleus 133 27 59 82 69 123 172 178 163 139 238 222 296 223 103 104 108 124 47 57 100 185 160
Nuclear Periphery 0 0 1 5 3 5 17 22 20 8 21 0 6 16 3 0 2 2 0 1 0 1 5
Nucleolus 19 6 15 22 26 41 117 111 107 104 160 14 28 51 10 14 25 24 3 16 23 90 95
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 2 0 0 0 2 4 3 1 1 0 0 3 0 0 0 7 1 5 2 6 14
Vac/Vac Membrane 10 4 5 5 9 6 23 18 18 10 13 6 26 39 19 27 37 2 2 7 10 54 70
Unique Cell Count 209 43 112 131 190 238 424 388 380 334 495 274 423 442 202 213 248 225 71 130 163 372 396
Labelled Cell Count 231 46 137 159 225 304 572 509 526 455 685 279 444 472 225 226 276 225 71 130 163 372 396


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 11.7 6.8 6.9 6.7 6.2 5.5 6.0 5.8 5.6 5.7 10.7 10.6 10.4 13.1 13.5 14.2 11.6 12.1 11.8
Std Deviation (1e-4) 2.2 2.3 1.8 1.8 1.8 1.8 1.8 1.9 2.1 1.8 1.7 2.0 2.1 1.9 3.0 2.8 2.9 2.5 2.6 2.1
Intensity Change (Log2) 0.01 -0.03 -0.14 -0.32 -0.19 -0.24 -0.28 -0.26 0.65 0.63 0.61 0.94 0.99 1.06 0.76 0.82 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.0 1.3 -2.8 -4.7 -4.8 -4.0 -4.8 -6.0 -4.9 -3.1 -1.0 0.5 0.6 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 0.9 2.9 5.1 2.7 4.5 4.8 4.5 0 0 0 -4.3 -5.7 -5.7
Nucleus 1.6 -2.8 -0.2 -2.3 -1.3 -1.8 -2.0 -0.9 5.7 3.4 -0.4 -0.3 -0.7 -1.6
Nuclear Periphery 0 0 0 0 2.1 0 0 0 0 0 0 0 0 0
Nucleolus 0.7 0.1 0.9 3.1 3.3 3.2 3.7 4.0 -2.8 -2.3 -0.5 -2.6 -2.0 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.1 0 0.4 0.1 0.1 0 0 0 0.7 1.5 1.6 2.4 2.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available