Standard name
Description Regulatory subunit B of protein phosphatase 2A (PP2A); Zds1p/2p-dependent localization to cytoplasm promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division, chromosome segregation during achiasmate meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; homolog of mammalian B55

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.96 0.96 0.91 0.88 0.77 0.82 0.8 0.79 0.72 0.74 0.97 0.99 0.99 0.95 0.93 0.91 0.93 0.95 0.88 0.94 0.88 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.06 0.18 0.24 0.17 0.19 0.26 0.4 0.24 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.07 0 0.06 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 1 3 0 7 6
Bud 1 5 6 3 3 2 5 10 3 4 4 3 3 3 1 2 1 2 0 1 2 0 5 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 3 2 2 6 6 6 7 7 7 4 7 0 7 2 17 18 18 0 0 0 0 0 0
Cytoplasm 155 230 164 163 253 272 306 352 204 222 105 110 321 596 550 829 486 320 167 233 157 223 399 327
Endoplasmic Reticulum 1 4 0 1 3 0 5 1 9 6 0 4 5 1 0 35 22 9 0 0 2 0 6 11
Endosome 0 0 0 1 2 1 1 1 1 0 0 0 0 2 3 15 3 6 0 1 1 3 8 29
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 6 10
Mitochondria 3 3 5 9 17 56 97 73 49 72 58 36 1 0 1 37 20 18 1 1 2 1 2 6
Nucleus 1 0 0 0 2 2 4 6 5 6 2 2 3 3 1 0 5 2 0 2 0 1 0 0
Nuclear Periphery 1 0 0 2 0 1 1 1 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 0 0 1 0 1 1 0 1 1 0 0 0 0 0 2 1 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 0 0 0 0 0 3 5
SpindlePole 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 4 3 2 0 0 0 0 7 10
Vac/Vac Membrane 0 0 1 2 10 4 23 29 7 18 4 7 2 0 0 23 29 11 0 0 4 1 0 7
Unique Cell Count 162 237 170 169 278 309 399 427 255 281 146 149 330 600 556 872 520 351 180 245 179 237 453 438
Labelled Cell Count 165 246 179 184 298 346 448 481 286 338 177 169 335 612 560 968 591 389 180 245 179 237 453 438


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 7.1 6.0 5.9 5.9 5.6 4.8 5.8 6.0 5.5 5.8 6.3 8.4 8.0 7.6 6.3 7.0 7.8 8.0 7.8 8.3
Std Deviation (1e-4) 0.9 0.9 1.0 1.1 1.5 1.2 1.0 1.2 1.5 1.4 1.5 1.8 1.2 1.1 1.1 1.6 1.7 2.0 1.6 1.4 1.4
Intensity Change (Log2) -0.04 -0.02 -0.1 -0.33 -0.05 -0.01 -0.14 -0.05 0.06 0.48 0.42 0.33 0.07 0.21 0.37 0.41 0.37 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Cytoplasm 0 -2.2 -3.1 -5.7 -4.5 -4.9 -5.1 -6.1 -5.8 0.5 2.9 2.2 -0.8 -1.5 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.7 2.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 1.5 4.8 0 4.6 4.9 6.2 8.2 5.6 0 0 0 0.8 0.5 1.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.8 3.1 0 3.0 0 0 0 0 0 0 2.8 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc55-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available